Intrusion-extrusion experiments of MgCl2 aqueous solution in pure silica ferrierite: Evidence of the nature of intruded liquid by in situ high pressure synchrotron X-ray powder diffraction

Experimental intrusion-extrusion isotherms of MgCl221H2O solution were recorded at room temperature on pure silica FER-type zeolite (Si-FER). The intrusion occurs at 195 MPa and the phenomenon is completely reversible with a slight hysteresis. The \u201cSi-FER - MgCl2 aqueous solution\u201d system behaves like a spring. The material was deeply characterized before and after intrusion-extrusion experiments and no significant changes were observed. The unit cell parameters were refined e on the basis of the in situ synchrotron X-ray powder diffraction data - up to 1.47 GPa and then at Pamb upon pressure release. The Rietveld refinement of the data collected at 0.28 GPa (280 MPa), a pressure close to the intrusion value, shows that both ions and water molecules present in the MgCl2 aqueous solution were intruded in the porosities. However, the solvation degree of the intruded ions differs from the initial solution, revealing a partial desolvation of both magnesium and chloride ions. As a whole, the nature and amount of the intruded species correspond to a MgCl210H2O composition. Moreover, at a higher pressure (0.68 GPa), a phase transition from the orthorhombic Pmnn to the monoclinic P21/n s.g. is observed in Si-FER. At 1.47 GPa, the zeolite maintains this monoclinic symmetry, while another phase transition, to the monoclinic P21 s g., is argued from the analysis of the pattern of the sample compressed to 2.6 GPa and then collected upon pressure release to ambient conditions

Characterization and dynamics of specific T cells against nucleophosmin-1 (NPM1)-mutated peptides in patients with NPM1-mutated acute myeloid leukemia

Nucleophosmin(NPM1)-mutated protein, a leukemia-specific antigen, represents an ideal target for AML immunotherapy. We investigated the dynamics of NPM1-mutated-specific T cells on PB and BM samples, collected from 31 adult NPM1-mutated AML patients throughout the disease course, and stimulated with mixtures of 18 short and long peptides (9-18mers), deriving from the complete C-terminal of the NPM1-mutated protein. Two 9-mer peptides, namely LAVEEVSLR and AVEEVSLRK (13.9-14.9), were identified as the most immunogenic epitopes. IFNγ-producing NPM1-mutated-specific T cells were observed by ELISPOT assay after stimulation with peptides 13.9-14.9 in 43/85 (50.6%) PB and 34/80 (42.5%) BM samples. An inverse correlation between MRD kinetics and anti-leukemic specific T cells was observed. Cytokine Secretion Assays allowed to predominantly and respectively identify Effector Memory and Central Memory T cells among IFNγ-producing and IL2-producing T cells. Moreover, NPM1-mutated-specific CTLs against primary leukemic blasts or PHA-blasts pulsed with different peptide pools could be expanded ex vivo from NPM1-mutated AML patients or primed in healthy donors. We describe the spontaneous appearance and persistence of NPM1-mutated-specific T cells, which may contribute to the maintenance of long-lasting remissions. Future studies are warranted to investigate the potential role of both autologous and allogeneic adoptive immunotherapy in NPM1-mutated AML patients

Effects of estrogens and oxytocin on the development of neonatal mammalian ovary

The preservation and death of germ cells in the neonatal mammalian ovary are linked with the presence of hormones. Estrogens and oxytocin are present at birth in all mammalian vertebrates. The aim of this study was to examine their role in the development of the neonatal ovary and also in the preservation and death of germ cells in the neonatal period: apoptotic phenomena play a fundamental role in the control of their number. Female neonatal mice were treated at birth with estradiol monobenzoate or oxytocin and sacrificed after 5 days. The ovaries were sectioned in toto into semi-thin sections, in order to calculate their volume. Thin sections were also carried out to verify, under the transmission electron microscope (T.E.M.), the cells in apoptosis. The ovaries treated with the greater concentration of estradiol monobenzoate showed a volume that was significantly greater than that of the controls and a reduction of germ cells in apoptosis. The ovaries treated with oxytocin at all degrees of concentration had a volume significantly less than the controls and they also had a higher number of germ cells in apoptosis

Ion exchange capacity of synthetic zeolite L: a promising way for cerium recovery

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Influence of estrogens and oxytocin on germ cells death in the neonatal mammalian ovary

During mammalian oogenesis, some processes involve proliferation and others drastic reduction of germ cells. This study reports on the role played by two hormones, estradiol monobenzoate and oxytocin, in the control of the number of germ cells in the neonatal mouse ovary. Female neonatal mice were treated with doses ranging between 0.1 and 1 microg/mouse of estradiol monobenzoate or oxytocin and sacrificed at 5 days of postnatal age. The results showed that in the animals treated with estrogen, follicular development was more advanced than that of controls. Further the number of germ cells in apoptosis was drastically reduced. In the animals treated with oxytocin, the follicular development was arrested at the stage of primary follicles. In addition, the number of apoptotic germ cells increased if compared with that of the controls

An Asymptomatic, Iatrogenic Hemobilia Detected by Contrast Enhanced Ultrasound of the Gallbladder

Hemorrhage within the biliary system (hemobilia), is an infrequent complication that can arise during percutaneous procedures involving the liver. The clinical diagnosis of hemobilia is often challenging, as symptoms are quite unspecific and often display a late onset. In such cases, sonography of the gallbladder can play a crucial role in the early detection of hemobilia. A case report is provided of a 72-year-old man who underwent a sonography-guided percutaneous biopsy of a 22-mm focal lesion, within the eighth segment of the liver. In this patient, asymptomatic active hemobilia was promptly identified through contrast-enhanced ultrasound (CEUS) which was performed immediately after the invasive procedure. Crucially, this postprocedural complication may have gone undiagnosed without an early postprocedure grayscale visual analysis and CEUS evaluation, since the patient was completely asymptomatic. In conclusion, a grayscale sonogram of the gallbladder is suggested and should be considered before and immediately after percutaneous invasive procedures of the liver. In the instances where suspicion of hemobilia arises, CEUS can be used to assess the presence of active bleeding. Implementing this type of imaging protocol may possibly reveal a higher frequency of hemobilia than is commonly reported

Maternal phthalate exposure and dietary habits during pregnancy: what do we need to look for?

Background and Objectives: Phthalates are chemical products widely used as plasticizers, potentially harmful, especially for their antiandrogenic effects in specific populations, such as pregnant women and newborns. Ingestion is an important exposure pathway, however specific exposure sources and drivers are mainly unknown. The aim of this study was to evaluate the association between dietary and cooking habits during pregnancy and phthalates exposure at delivery. Methods: 197 women were recruited after delivery, in a single-center prospective cohort study at Modena’s University Hospital (Italy), from January 2019 to October 2020. Exposure to 6 phthalates was assessed in urine samples and expressed as µg/g of urinary creatinine. Pregnancy, dietary and cooking habits were recorded by ad hoc questionnaire. Descriptive statistics and bivariate analyzes were performed. Results: Overall, exposure resulted widespread in women at delivery. In women usually boiling food, urinary levels of Monobenzylphthalate (MBzP) (3.4±2 vs 4.7±3.3 μg/g) and Mono-2-ethylhexylphthalate (MEHP) (2.3±1.8 vs 5.2±15.2 μg/g) were significantly lower, whereas in women usually frying food, monon-butylphthalate (MnBP) appeared significantly higher (23.2±59.6 vs 9.8±11.5 μg/g). Low fruit consumption during pregnancy was associated with higher MnBP concentrations compared to daily consumption (39.3±83.1 vs 10.6±11.5 μg/g). Concentrations of monoethylphthalate (MEP) appeared higher in women who consumed cheese, eggs and fish. Daily consumption of red meat, beef and pork was associated with higher levels of di(2-ethylhexyl)phthalate (DEHP) metabolites. Conclusion: No strong association among exposure and dietary habits was observed, even though urinary levels of some phthalates resulted higher in women frequently consuming fat food and lower when consuming fruits or choosing healthier cooking methods. Surprisingly, no correlation appeared with packaged foods and packaging material. Further studies are needed to better elucidate ingestion exposure pathways with a greater focus on alternative sources of contamination such as food production, processing and transportation to better guide evidence-based policy making