Sub-Telomeric core X and Y' Elements in S.cerevisiae Suppress Extreme Variations in Gene Silencing

Telomere Position Effect (TPE) is governed by strong repression signals emitted by telomeres via the Sir2/3/4 Histone Deacetylase complex. These signals are then relayed by weak proto-silencers residing in the subtelomeric core X and Y' elements. Subtelomeres also contain Sub-Telomeric Anti-silencing Regions (STARs). In this study we have prepared telomeres built of different combinations of core X, Y' and STARs and have analyzed them in strains lacking Histone-Acetyltransferase genes as well as in cdc6-1 and Δrif1 strains. We show that core X and Y' dramatically reduce both positive and negative variations in TPE, that are caused by these mutations. We also show that the deletion of Histone-Acetyltransferase genes reduce the silencing activity of an ACS proto-silencer, but also reduce the anti-silencing activity of a STAR. We postulate that core X and Y' act as epigenetic “cushioning” cis-elements

Bollywood's Global Reach: Consuming the Diasporic Consciousness

Using the British Sikh community as its research context, this article explores the influence of the Bollywood film genre on what Vertovic refers to as the “diasporic consciousness” in relation to this community. Bollywood attempts to speak to the diaspora by conveying a new sense of “Indian-ness,” one that is less about citizenship and more about imagined identity and community. The authors investigate what they have termed the “Indian imaginary” and how the values embedded therein impact on the lives of young British Sikhs. The findings discuss three emergent core themes: (1) reaffirming pride in Indian heritage; (2) evoking romance and longing; and (3) reinforcing family values and a sense of kinship within the British Sikh diaspora. The overall contribution of the article is twofold. First, it illustrates how the globalization of Bollywood affects the Indian diaspora at a local level. Second, it shows how Bollywood provides an important space for negotiating and reconciling various tensions between family-based and more individualistic value systems. Ultimately, then, Bollywood offers young British Sikhs a particular, hybridized representation of courtship and marriage that is both romantic and familial, and that serves to reconcile Eastern and Western marital relationship ideals and oppositional cultural discourses

Ashis Nandy e as vicissitudes do self: crítica, subjetividade e civilização indiana

Este texto trata da obra de Ashis Nandy, um dos principais intelectuais indianos contemporâneos, psicólogo social, psicanalista, bem como cientista político, que busca desenvolver o que chamou de "tradicionalismo crítico". Sua discussão da personalidade e do indivíduo, dentro de uma concepção ampla da civilização da Índia, com a modernidade fazendo-se presente como ameaça explícita (e elemento implícito, mais ambiguamente), é crucial em vários sentidos - em particular teoricamente - para o entendimento da Índia e para uma teoria da civilização. A composição múltipla do self e suas relações tanto com a cultura índica quanto com a ocidental se destacam em sua discussão.<br>This paper examines the work of Ashis Nandy, one of Indian's foremost contemporary intellectuals, a social psychologist, psychoanalyst and political scientist, responsible for developing what he calls a &#8216;critical traditionalism.' Adopting a broad conception of Indian civilization in which modernity poses an explicit threat (and, more ambiguously, an implicit element), Nandy's discussion of personality and the individual is crucial at various levels - in particular theoretical - to understanding India and developing a theory of civilization. One of the prominent features of his discussion is the multiple composition of the self and its relations to both Indian and Western civilizations

Evolution of genes and genomes on the Drosophila phylogeny

Affiliations des auteurs : cf page 216 de l'articleInternational audienceComparative analysis of multiple genomes in a phylogenetic framework dramatically improves the precision and sensitivity of evolutionary inference, producing more robust results than single-genome analyses can provide. The genomes of 12 Drosophila species, ten of which are presented here for the first time (sechellia, simulans, yakuba, erecta, ananassae, persimilis, willistoni, mojavensis, virilis and grimshawi), illustrate how rates and patterns of sequence divergence across taxa can illuminate evolutionary processes on a genomic scale. These genome sequences augment the formidable genetic tools that have made Drosophila melanogaster a pre-eminent model for animal genetics, and will further catalyse fundamental research on mechanisms of development, cell biology, genetics, disease, neurobiology, behaviour, physiology and evolution. Despite remarkable similarities among these Drosophila species, we identified many putatively non-neutral changes in protein-coding genes, non-coding RNA genes, and cis-regulatory regions. These may prove to underlie differences in the ecology and behaviour of these diverse species

Evolution of genes and genomes on the Drosophila phylogeny

Comparative analysis of multiple genomes in a phylogenetic framework dramatically improves the precision and sensitivity of evolutionary inference, producing more robust results than single-genome analyses can provide. The genomes of 12 Drosophila species, ten of which are presented here for the first time (sechellia, simulans, yakuba, erecta, ananassae, persimilis, willistoni, mojavensis, virilis and grimshawi), illustrate how rates and patterns of sequence divergence across taxa can illuminate evolutionary processes on a genomic scale. These genome sequences augment the formidable genetic tools that have made Drosophila melanogaster a pre-eminent model for animal genetics, and will further catalyse fundamental research on mechanisms of development, cell biology, genetics, disease, neurobiology, behaviour, physiology and evolution. Despite remarkable similarities among these Drosophila species, we identified many putatively non-neutral changes in protein-coding genes, non-coding RNA genes, and cis-regulatory regions. These may prove to underlie differences in the ecology and behaviour of these diverse species