Frequency of natural out-crossing in partially cleistogamous pigeonpea lines in diverse environments

Natural out-crossing is the major cause of loss of varietal purity in pegeonpea [Cajanus cajan (L.) Millsp.]. The frequency of natural out-crossing of partially cleistogamous mutant lines, characterized by a modified keel and filamentous anthers, was studied at two locations in Sri Lanka and three locations in India. Indeterminate growth habit and normal floral morphology were used as dominant markers and the frequency of anural out-crossing was estimated as percentage of the observed hybrid plants. Natural out-crossing in the mutant lines in Sri Lanka ranged from 0.14 to 1.33% in comparison to 6.34 to 19.64% in the controls. In the Indian environments, natural outcrossing ranged from 0.16 to 2.67%. The mutant was higly stable over diverse environments, and may be of considerable economic importance in pigeonpea improvement and seed-production programs

Recurrent Fusion Genes in Gastric Cancer: CLDN18-ARHGAP26 Induces Loss of Epithelial Integrity.

Genome rearrangements, a hallmark of cancer, can result in gene fusions with oncogenic properties. Using DNA paired-end-tag (DNA-PET) whole-genome sequencing, we analyzed 15 gastric cancers (GCs) from Southeast Asians. Rearrangements were enriched in open chromatin and shaped by chromatin structure. We identified seven rearrangement hot spots and 136 gene fusions. In three out of 100 GC cases, we found recurrent fusions between CLDN18, a tight junction gene, and ARHGAP26, a gene encoding a RHOA inhibitor. Epithelial cell lines expressing CLDN18-ARHGAP26 displayed a dramatic loss of epithelial phenotype and long protrusions indicative of epithelial-mesenchymal transition (EMT). Fusion-positive cell lines showed impaired barrier properties, reduced cell-cell and cell-extracellular matrix adhesion, retarded wound healing, and inhibition of RHOA. Gain of invasion was seen in cancer cell lines expressing the fusion. Thus, CLDN18-ARHGAP26 mediates epithelial disintegration, possibly leading to stomach H(+) leakage, and the fusion might contribute to invasiveness once a cell is transformed. Cell Rep 2015 Jul 14; 12(2):272-285

Irrigation versus hydropower : sectoral conflicts in southern Sri Lanka

Although hydropower does not directly consume water, its generation frequently conflicts with other uses, notably irrigation, because its release schedule does not always correspond to the timing of water use by other activities. This article analyses a case from the Walawe river basin, Sri Lanka, where economic efficiency can be raised by reducing releases from the dam for irrigation for the benefit of hydropower generation. The tradeoff is analysed in financial and managerial terms and different options for reducing irrigation diversions are reviewed. Although the high level of current diversions for irrigation warrants the possibility of improvement in management, it is shown that finding ways to reduce supply faces technical and socio-political constraints that make the realization of economic benefits costly and difficult

Effect of Plant Population on Yield and Yield Components in Main and Ratoon Crops of Pigeonpea in Sri Lanka

In a field experiment in 1990/91 in Sri Lanka, pigeonpeas cv. ICPL 2 were sown during the rainy season at densities of 4-66 plants/m². The crop was harvested on 14 March 1991 by pod picking and at the onset of postrainy season rains it was ratooned to a height of 45 cm. The highest combined yield of the main (1.77 t/ha) and the ratoon (1.93 t) crops was obtained at the density of 17 plants/m². Data on yield components are given

Maruca testulalis Damage in Determinate and Indeterminate Lines of Pigeonpea in Sri Lanka

To compare damage caused by M. testulalis [M. vitrata] in determinate (DT) and indeterminate (IDT) lines of pigeon pea [Cajanus cajan], 271 short-duration genotypes were evaluated for percentage pod damage, days to 50% flowering and recovery from pod damage at the Field Crops Research and Development Institute, Maha Illuppallama, Sri Lanka during 1990-91. Mean pod damage of DT and IDT lines was 65-75 and 40-50%, respectively and none of the lines had less than 10% damage. Number of days to 50% flowering in DT lines was 54-84 and was not related to pod damage. In IDT lines, days to 50% flowering was 60-87 days and had a significant negative correlation with M. testulalis damage (r = -0.43), showing that early flowering lines suffered more damage than late flowering lines. In general, pod damage by M. testulalis caused serious flower drop in both DT and IDT types. Recovery from pod damage was poor in DT lines but excellent recovery was recorded in IDT lines ICPL88034, ICPL87113 and MG679. DT lines appear to be more prone to M. testulalis damage because they have clustered inflorescences compared to IDT types which have long fruiting branches and loose inflorescences

Demonstration of antigenic polymorphism in Plasmodium vivax malaria with a panel of 30 monoclonal antibodies.

A panel of 30 monoclonal antibodies was established against asexual erythrocytic stages of Plasmodium vivax and used to investigate the antigenic composition of the parasite. At least 38 different antigenic polypeptides of P. vivax were characterized by the Western blot technique. The possible location of these antigens, as well as their stage and species specificity, was determined on the basis of the staining patterns produced by these antibodies on air-dried parasites in the indirect immunofluorescence test. Immunofluorescence performed with 30 different monoclonal antibodies on 50 different isolates of P. vivax obtained from patients showed that a high level of antigenic polymorphism prevailed in P. vivax. Only six monoclonal antibodies reacted with epitopes that were represented in more than 80% of parasite isolates, and therefore, appeared to be relatively conserved among different isolates. The other 24 monoclonal antibodies reacted with only 20 to 70% of parasite isolates

Aggressive strategies of the COVID-19 pandemic on the apparel industry of Sri Lanka using structural equation modeling

During the COVID-19 crisis, the apparel industry faced many challenges. Aggressive cost-cutting strategies became a top priority, and in turn, these influenced stressors and adversely affected business sustainability. This study examines the impact of aggressive strategies during the COVID-19 pandemic on business sustainability in the apparel industry of Sri Lanka. Further, it investigates whether the relationship between aggressive cost-cutting strategies and business sustainability was mediated by employee stress, considering aggressive cost reduction strategies and workplace environmental changes. This was a cross-sectional study with data collected from 384 employees in the apparel industry in Sri Lanka. Structural Equation Modelling (SEM) was applied to analyze the direct and indirect effects of aggressive cost reduction strategies and workplace environmental changes on sustainability with mediating effects of stress. Aggressive cost reduction strategies (Beta = 1.317, p = 0.000) and environmental changes (Beta = 0.251, p = 0.000) led to an increase in employee stress but did not affect business sustainability. Thus, employee stress (Beta = -0.028, p = 0.594) was not a mediator in the relationship between aggressive cost-cutting strategies and business sustainability; business sustainability was not a dependent variable. The findings proved that managing workplace stress, particularly improving stressful working environments and aggressive cost reduction strategies, can enhance employee satisfaction. Thus, managing employee stress could be beneficial for policymakers to focus on the area(s) required to retain competent employees. Moreover, aggressive strategies are unsuitable to apply during crisis to enhance business sustainability. The findings provide additional knowledge to the existing literature, enabling employees and employers to predict causes of stress and serve as a significant knowledge base for further studies

Aggressive strategies of the COVID-19 pandemic on the apparel industry of Sri Lanka using structural equation modeling.

During the COVID-19 crisis, the apparel industry faced many challenges. Aggressive cost-cutting strategies became a top priority, and in turn, these influenced stressors and adversely affected business sustainability. This study examines the impact of aggressive strategies during the COVID-19 pandemic on business sustainability in the apparel industry of Sri Lanka. Further, it investigates whether the relationship between aggressive cost-cutting strategies and business sustainability was mediated by employee stress, considering aggressive cost reduction strategies and workplace environmental changes. This was a cross-sectional study with data collected from 384 employees in the apparel industry in Sri Lanka. Structural Equation Modelling (SEM) was applied to analyze the direct and indirect effects of aggressive cost reduction strategies and workplace environmental changes on sustainability with mediating effects of stress. Aggressive cost reduction strategies (Beta = 1.317, p = 0.000) and environmental changes (Beta = 0.251, p = 0.000) led to an increase in employee stress but did not affect business sustainability. Thus, employee stress (Beta = -0.028, p = 0.594) was not a mediator in the relationship between aggressive cost-cutting strategies and business sustainability; business sustainability was not a dependent variable. The findings proved that managing workplace stress, particularly improving stressful working environments and aggressive cost reduction strategies, can enhance employee satisfaction. Thus, managing employee stress could be beneficial for policymakers to focus on the area(s) required to retain competent employees. Moreover, aggressive strategies are unsuitable to apply during crisis to enhance business sustainability. The findings provide additional knowledge to the existing literature, enabling employees and employers to predict causes of stress and serve as a significant knowledge base for further studies

Model fitting analysis.

During the COVID-19 crisis, the apparel industry faced many challenges. Aggressive cost-cutting strategies became a top priority, and in turn, these influenced stressors and adversely affected business sustainability. This study examines the impact of aggressive strategies during the COVID-19 pandemic on business sustainability in the apparel industry of Sri Lanka. Further, it investigates whether the relationship between aggressive cost-cutting strategies and business sustainability was mediated by employee stress, considering aggressive cost reduction strategies and workplace environmental changes. This was a cross-sectional study with data collected from 384 employees in the apparel industry in Sri Lanka. Structural Equation Modelling (SEM) was applied to analyze the direct and indirect effects of aggressive cost reduction strategies and workplace environmental changes on sustainability with mediating effects of stress. Aggressive cost reduction strategies (Beta = 1.317, p = 0.000) and environmental changes (Beta = 0.251, p = 0.000) led to an increase in employee stress but did not affect business sustainability. Thus, employee stress (Beta = -0.028, p = 0.594) was not a mediator in the relationship between aggressive cost-cutting strategies and business sustainability; business sustainability was not a dependent variable. The findings proved that managing workplace stress, particularly improving stressful working environments and aggressive cost reduction strategies, can enhance employee satisfaction. Thus, managing employee stress could be beneficial for policymakers to focus on the area(s) required to retain competent employees. Moreover, aggressive strategies are unsuitable to apply during crisis to enhance business sustainability. The findings provide additional knowledge to the existing literature, enabling employees and employers to predict causes of stress and serve as a significant knowledge base for further studies.</div

Regression weight estimate values among variables.

Source: Author’s illustration based on AMOS result.</p