In vitro cytotoxic and antioxidant properties of the aqueous, chloroform and methanol extracts of Dicranopteris linearis leaves

The in vitro cytotoxic and antioxidant properties of the aqueous, chloroform and methanol extracts of the Dicranopteris linearis leaves were investigated in the present study. The cytotoxic effect was determined against the normal (3T3) and cancer cells’ lines (MCF-7, HeLa, HT-29, HL-60, K-562 and MDA-MB-231) using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay, while the antioxidant activity was assessed using the DPPH radical and superoxide scavenging assays. Based on the results obtained, the aqueous extract was not effective against any of the types of cancer cells studied; the chloroform extract was effective only against MCF-7 and HeLa; and the methanol extract was effective against all the cancer cells used. Interestingly, all extracts failed to produce cytotoxic effect against the 3T3 cells (normal cell) indicating their safety. All extracts (20, 100 and 500 μg/ml) were found to exert antioxidant activity when tested using the DPPH radical and superoxide scavenging assays; with the methanol, followed by the aqueous and chloroform extracts exhibiting the highest antioxidant activity in both assays. The total phenolic content for the aqueous, methanol and chloroform extracts were 3112.1 ± 6.7, 3417.3 ± 4.7 and 1012.7 ± 5.3 mg/100 g gallic acid, respectively. In conclusion, the leaves of D. linearis possess potential cytotoxic activity against various types of cancer cell lines depending on the types of extracts used and antioxidant activity, which need to be further explored.Keywords: Dicranopteris linearis, in vitro anticancer activity, MTT assay, aqueous extract, chloroform extract, methanol extrac

ANALISIS KEBERTERIMAAN PROGRAM APLIKASI SEKOLAH (PAS) SMK VERSI 2.0 DENGAN EXTENDED TAM DI KABUPATEN BANTUL

The use of PAS SMK Version 2.0 new beginning in the academic year 2013/1014, so it is necessary to know how the research dilakukakan user acceptance for this new application. This study adds two external variables, namely top management support and computer self efficacy The focus of this study was to test the existing variables in the TAM model by adding external variables and Principal Support Computer Confidence is believed to influence the behavior intention PAS uses version 2.0 of CMS. The study included the entire population of data collection officer in Bantul district as many as 44 data collection officer in every school. Suitability models and hypothesis testing performed using analysis of Structural Equation Model (SEM) with Smart-Partial Least Square (Smart-PLS). Seven of the ten hypotheses tested proven at 0.05 and 0.01 significance level. The results showed that the variables of perceived usefulness and perceived ease of use are the variables that direct positive effect on behavioral intentions PAS use CMS Version 2.0. While variavel top management support has no significant effect on perceived usefulness and perceived ease of use of this application, however, has a significant effect on the computer self efficacy