Spontaneous abortion and ocular toxoplasmosis in a couple infected with Toxoplasma gondii

Aims: To report the cases of wife and husband with toxoplasmosis. The woman acquired the infection during pregnancy while her husband was diagnosed with ocular toxoplasmosis 15 years later. Cases report: A woman with twin pregnancy, living in southern Brazil, was seronegative for Toxoplasma gondii IgG and IgM antibodies in the first month of gestation. Seroconversion (positive T. gondii IgM and IgG antibodies) occurred in the seventh week of gestation, resulting in spontaneous abortion. Fifteen years later, her husband presented with eye irritation and blurred vision. The funduscopic examination revealed typical lesions of recurrent chorioretinitis in the right eye. The serological tests were positive for T. gondii IgG and negative for IgM. Conclusions: This report of a couple with important clinical outcomes of two different types of toxoplasmosis underscores the importance of this disease which, in its various manifestations, may have serious consequences for a family's life

Association of cytokines in individuals sensitive and insensitive to dust mites in a Brazilian population.

Allergic reaction to dust mites is a relatively common condition among children, triggering cutaneous and respiratory responses that have a great impact on the health of this population. Anaphylactic hypersensitivity is characterized by an exacerbated response involving the production of regulatory cytokines responsible for stimulating the production of IgE antibodies.To investigate an association of variants in cytokine genes (IL1A-889, IL1B-511, +3962, IL1R1970, IL1RA11100, IL4RA+1902, IL12-1188, IFNG+874, TGFB1 codon 10, codon 25, TNFA-308, -238, IL2-330, +166, IL4-1098, -590, -33, IL6-174, nt565, and IL10-1082, -819, -592) between patients sensitive to dust mites and a control group.A total of 254 patients were grouped as atopic and non-atopic according to sensitivity as evaluated by the Prick Test and to cytokine genotyping by the polymerase chain reaction-sequence specific primers (PCR-SSP) method using the Cytokine Genotyping Kit.A comparison between individuals allergic to Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Blomia tropicalis and a non-atopic control group showed significant differences between allele and genotype frequencies in the regulatory regions of cytokine genes, with important evidence for IL4-590 in T/C (10.2% vs. 43.1%, odd ratio [OR] = 0.15, p = 5.2 10-8, pc = 0.0000011, and 95% confidence interval [95%CI] = 0.07-0.32) and T/T genotypes (42.9% vs. 13.8%, OR = 4.69, p = 2.5 10-6, pc = 0.000055, and 95%CI = 2.42-9.09). Other associations were observed in the pro-inflammatory cytokines IL1A-889 (T/T, C, and T) and IL2-330 (G/T and T/T) and the anti-inflammatory cytokines IL4RA+1902 (A and G), IL4-590 (T/C, T/T, C, and T), and IL10-592 (A/A, C/A, A, and C).Our results suggest a possible association between single nucleotide polymorphisms (SNPs) in cytokine genes and hypersensitivity to dust mites

Significant allele, genotype and haplotype frequencies of cytokine SNPs in individuals allergic to dust mite 3 (<i>Blomia tropicalis</i>) and controls.

<p>SNPs  =  single nucleotide polymorphisms; p =  Fisher's exact test with Yates' correction; pc =  after Bonferroni correction; ns =  non-significant; OR =  odds ratio; 95%CI = 95% confidence interval.</p><p>Significant allele, genotype and haplotype frequencies of cytokine SNPs in individuals allergic to dust mite 3 (<i>Blomia tropicalis</i>) and controls.</p

Cytokine genes associated with different diseases as reported in previous studies.

<p>SNP  =  single nucleotide polymorphism.</p><p>Cytokine genes associated with different diseases as reported in previous studies.</p

Significant allele, genotype and haplotype frequencies of cytokine SNPs in individuals allergic to at least one of the following dust mites: <i>Dermatophagoides farinae</i>, <i>Dermatophagoides pteronyssinus</i>, and <i>Blomia tropicalis</i>, and controls.

<p>SNPs  =  single nucleotide polymorphisms; p =  Fisher's exact test with Yates' correction; pc =  after Bonferroni correction; ns =  non-significant; OR =  odds ratio; 95%CI = 95% confidence interval.</p><p>Significant allele, genotype and haplotype frequencies of cytokine SNPs in individuals allergic to at least one of the following dust mites: <i>Dermatophagoides farinae</i>, <i>Dermatophagoides pteronyssinus</i>, and <i>Blomia tropicalis</i>, and controls.</p

Results of age- and sex-adjusted logistic regression analysis.

<p>OR  =  odds ratio; Ref  =  reference; 95%CI  = 95% confidence interval;</p><p>* Significant at p<0.05.</p><p>Results of age- and sex-adjusted logistic regression analysis.</p

Room, middle

room nThis property west of Pethricks called the middle Room. .was used in the summer by Planters from Bay Roberts . . .JH 11/75Used I and SupNot used1Not use