28.直腸カルチノイド(第609回千葉医学会例会・第1外科教室談話会)

Basic PELE-seq SNP-calling scripts. The basic commands used for PELE-Seq analysis are provided as a shell script. (TXT 11 kb

Additional file 5: of High-specificity detection of rare alleles with Paired-End Low Error Sequencing (PELE-Seq)

PCR errors that result in false positive SNPs when using unfiltered ORP data. Overlapping paired-end read libraries sequenced to 10,000× OPE depth contained 109 false positive SNPs when rare variants were called with Lofreq, using default parameters with no minimum allele frequency cutoff. These putative PCR errors are predominately C > T transitions are are found in distinct clusters throughout the genome. This data is also included as a spreadsheet in Additional file 3. (PDF 18 kb