Direct measurement of the mechanical work during translocation by the ribosome.

A detailed understanding of tRNA/mRNA translocation requires measurement of the forces generated by the ribosome during this movement. Such measurements have so far remained elusive and, thus, little is known about the relation between force and translocation and how this reflects on its mechanism and regulation. Here, we address these questions using optical tweezers to follow translation by individual ribosomes along single mRNA molecules, against an applied force. We find that translocation rates depend exponentially on the force, with a characteristic distance close to the one-codon step, ruling out the existence of sub-steps and showing that the ribosome likely functions as a Brownian ratchet. We show that the ribosome generates ∼13 pN of force, barely sufficient to unwind the most stable structures in mRNAs, thus providing a basis for their regulatory role. Our assay opens the way to characterizing the ribosomes full mechano-chemical cycle

Suppression of inhomogeneous broadening in rf spectroscopy of optically trapped atoms

We present a novel method for reducing the inhomogeneous frequency broadening in the hyperfine splitting of the ground state of optically trapped atoms. This reduction is achieved by the addition of a weak light field, spatially mode-matched with the trapping field and whose frequency is tuned in-between the two hyperfine levels. We experimentally demonstrate the new scheme with Rb 85 atoms, and report a 50-fold narrowing of the rf spectrum

Drum vortons in high density QCD

Recently it was shown that high density QCD supports of number of topological defects. In particular, there are U(1)_Y strings that arise due to K^0 condensation that occurs when the strange quark mass is relatively large. The unique feature of these strings is that they possess a nonzero K^+ condensate that is trapped on the core. In the following we will show that these strings (with nontrivial core structure) can form closed loops with conserved charge and currents trapped on the string worldsheet. The presence of conserved charges allows these topological defects, called vortons, to carry angular momentum, which makes them classically stable objects. We also give arguments demonstrating that vortons carry angular momentum very efficiently (in terms of energy per unit angular momentum) such that they might be the important degrees of freedom in the cores of neutron stars.Comment: 11 pages, accepted for publication in Physical Review

Vortons in the SO(5) model of high temperature superconductivity

It has been shown that superconducting vortices with antiferromagnetic cores arise within Zhang's SO(5) model of high temperature supercondictivity. Similar phenomena where the symmetry is not restored in the core of the vortex was discussed by Witten in the case of cosmic strings. It was also suggested that such strings can form stable vortons, which are closed loops of such vortices. Motivated by this analogy, in following we will show that loops of such vortices in the SO(5) model of high T_c superconductivity can exist as classically stable objects, stabilized by the presence of conserved charges trapped on the vortex core. These objects carry angular momentum which counteracts the effect of the string tension that causes the loops to shrink. The existence of such quasiparticles, which are called vortons, could be interesting for the physics of high temperature superconductors. We also speculate that the phase transition between superconducting and antiferromagnetic phases at zero external magnetic field when the doping parameter changes is associated with vortons.Comment: 11 page

Global Strings in High Density QCD

We show that several types of global strings occur in colour superconducting quark matter due to the spontaneous violation of relevant U(1) symmetries. These include the baryon U(1)_B, and approximate axial U(1)_A symmetries as well as an approximate U(1)_S arising from kaon condensation. We discuss some general properties of these strings and their interactions. In particular, we demonstrate that the U(1)_A strings behave as superconducting strings. We draw some parallels between these strings and global cosmological strings and discuss some possible implications of these strings to the physics in neutron star cores.Comment: LaTeX JHEP-format (26 pages) Option in source for REVTeX4 forma

Development of targeted therapy for ovarian cancer mediated by a plasmid expressing diphtheria toxin under the control of H19 regulatory sequences

<p>Abstract</p> <p>Background</p> <p>Ovarian cancer ascites fluid (OCAF), contains malignant cells, is usually present in women with an advanced stage disease and currently has no effective therapy. Hence, we developed a new therapy strategy to target the expression of diphtheria toxin gene under the control of H19 regulatory sequences in ovarian tumor cells. H19 RNA is present at high levels in human cancer tissues (including ovarian cancer), while existing at a nearly undetectable level in the surrounding normal tissue.</p> <p>Methods</p> <p>H19 gene expression was tested in cells from OCAF by the in-situ hybridization technique (ISH) using an H19 RNA probe. The therapeutic potential of the toxin vector DTA-H19 was tested in ovarian carcinoma cell lines and in a heterotopic animal model for ovarian cancer.</p> <p>Results</p> <p>H19 RNA was detected in 90% of patients with OCAF as determined by ISH. Intratumoral injection of DTA-H19 into ectopically developed tumors caused 40% inhibition of tumor growth.</p> <p>Conclusion</p> <p>These observations may be the first step towards a major breakthrough in the treatment of human OCAF, while the effect in solid tumors required further investigation. It should enable us to identify likely non-responders in advance, and to treat patients who are resistant to all known therapies, thereby avoiding treatment failure.</p