35 research outputs found
Comparative Transcriptome Analysis of Milk Somatic Cells During Lactation Between Two Intensively Reared Dairy Sheep Breeds
In dairy sheep industry, milk production dictates the value of a ewe. Milk production is directly related to the morphology and physiology of the mammary gland; both being designated targets of breeding strategies. Although within a flock breeding parameters are mutual, large differences in milk production among individual ewes are usually observed. In this work, we tested two of the most productive dairy sheep breeds reared intensively in Greece, one local the Chios breed and one foreign the Lacaune breed. We used transcriptome sequencing to reveal molecular mechanisms that render the mammary gland highly productive or not. While highly expressed genes (caseins and major whey protein genes) were common among breeds, differences were observed in differentially expressed genes. ENSOARG00000008077, as a member of ribosomal protein 14 family, together with LPCAT2, CCR3, GPSM2, ZNF131, and ASIP were among the genes significantly differentiating mammary glandâs productivity in high yielding ewes. Gene ontology terms were mainly linked to the inherent transcriptional activity of the mammary gland (GO:0005524, GO:0030552, GO:0016740, GO:0004842), lipid transfer activity (GO:0005319) and innate immunity (GO:0002376, GO:0075528, GO:0002520). In addition, clusters of genes affecting zinc and iron trafficking into mitochondria were highlighted for high yielding ewes (GO:0071294, GO:0010043). Our analyses provide insights into the molecular pathways involved in lactation between ewes of different performances. Results revealed management issues that should be addressed by breeders in order to move toward increased milk yields through selection of the desired phenotypes. Our results will also contribute toward the selection of the most resilient and productive ewes, thus, will strengthen the existing breeding systems against a spectrum of environmental threats
Microbiota and volatilome profile of fresh and chill-stored deepwater rose shrimp (Parapenaeus longirostris)
Diet supplementation with fishâderived extracts suppresses diabetes and modulates intestinal microbiome in a murine model of dietâinduced obesity
Metabolic syndrome-related diseases affect millions of people worldwide. It is well established that changes in nutritional habits and lifestyle can improve or prevent metabolic-related pathologies such as type-2 diabetes and obesity. Previous reports have shown that nutritional supplements have the capacity to limit glucose intolerance and suppress diabetes development. In this study, we investigated the effect of dietary supplementation with fish-derived extracts on obesity and type 2 diabetes and their impact on gut microbial composition. We showed that nutritional supplements containing Fish Complex (FC), Fish Complex combined with Cod Powder (FC + CP), or Cod Powder combined with Collagen (CP + C) improved glucose intolerance, independent of abdominal fat accumulation, in a mouse model of diet-induced obesity and type 2 diabetes. In addition, collagen-containing supplements distinctly modulate the gut microbiome in high-fat induced obesity in mice. Our results suggest that fish-derived supplements suppress diet-induced type 2 diabetes, which may be partly mediated through changes in the gut microbiome. Thus, fish-derived supplements and particularly the ones containing fish collagen have potential beneficial properties as dietary supplements in managing type 2 diabetes and metabolic syndrome via modulation of the gut microbiome.publishedVersio
Diet supplementation with fishâderived extracts suppresses diabetes and modulates intestinal microbiome in a murine model of dietâinduced obesity
Metabolic syndrome-related diseases affect millions of people worldwide. It is well established that changes in nutritional habits and lifestyle can improve or prevent metabolic-related pathologies such as type-2 diabetes and obesity. Previous reports have shown that nutritional supplements have the capacity to limit glucose intolerance and suppress diabetes development. In this study, we investigated the effect of dietary supplementation with fish-derived extracts on obesity and type 2 diabetes and their impact on gut microbial composition. We showed that nutritional supplements containing Fish Complex (FC), Fish Complex combined with Cod Powder (FC + CP), or Cod Powder combined with Collagen (CP + C) improved glucose intolerance, independent of abdominal fat accumulation, in a mouse model of diet-induced obesity and type 2 diabetes. In addition, collagen-containing supplements distinctly modulate the gut microbiome in high-fat induced obesity in mice. Our results suggest that fish-derived supplements suppress diet-induced type 2 diabetes, which may be partly mediated through changes in the gut microbiome. Thus, fish-derived supplements and particularly the ones containing fish collagen have potential beneficial properties as dietary supplements in managing type 2 diabetes and metabolic syndrome via modulation of the gut microbiome.publishedVersio
Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes
Background: Salvia diterpenes have been found to have health promoting
properties. Among them, carnosic acid and carnosol, tanshinones and
sclareol are well known for their cardiovascular, antitumor,
antiinflammatory and antioxidant activities. However, many of these
compounds are not available at a constant supply and developing
biotechnological methods for their production could provide a
sustainable alternative. The transcriptome of S. pomifera glandular
trichomes was analysed aiming to identify genes that could be used in
the engineering of synthetic microbial systems.
Results: In the present study, a thorough metabolite analysis of S.
pomifera leaves led to the isolation and structure elucidation of
carnosic acid-family metabolites including one new natural product.
These labdane diterpenes seem to be synthesized through miltiradiene and
ferruginol. Transcriptomic analysis of the glandular trichomes from the
S. pomifera leaves revealed two genes likely involved in miltiradiene
synthesis. Their products were identified and the corresponding enzymes
were characterized as copalyl diphosphate synthase (SpCDS) and
miltiradiene synthase (SpMilS). In addition, several CYP-encoding
transcripts were identified providing a valuable resource for the
identification of the biosynthetic mechanism responsible for the
production of carnosic acid-family metabolites in S. pomifera.
Conclusions: Our work has uncovered the key enzymes involved in
miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The
transcriptomic dataset obtained provides a valuable tool for the
identification of the CYPs involved in the synthesis of carnosic
acid-family metabolites.General Secretariat of Research and Technology (GSRT) {[}09-SYN-23-879];
grant SEE-ERA. NET PLUS {[}ERA 64/01]; grant KRIPIS {[}MIS 448840
An exploration of agro-food chain distributive, procedural and interactional fairness in food products
Fairness and ethical values in agro-food chain lay at the center of the current debate on agro-food chain sustainability. The food labels may contain elements focused on agro-food chain management fairness practices. The objective of the present study is to explore what types of fairness agro-food companies value in the food products they commercialize, and if the characteristics of agro-food companies have a relationship with the type of fairness valued. The research study collected 226 commercialized food products containing information on the agro-food chain fairness practices companies adopted to produce the food products. Data elaboration included a cluster analysis to identify groups of fair products, and a multinomial logistic regression to explore the relationship between the identified clusters and fairness types, organic, nutritional and functional claims, and price. Results support that fairness-oriented products provide information on different types of fairness that is distributive, procedural, and interactional. Some products provide economic information on the distribution of price between upstream and downstream actors. Other products focus on procedural and interactional fairness practices, such as dignity, respect and transparent relationship in agro-food chain; technologies used in the production and distribution of the product; and ethical certifications that companies hold. Fairness-oriented information differ depending both on the type of chain actor commercializing the product, and on the type of brand, that is whether commercial brand or private label. The main conclusion is that companies convey fairness-related information to consumers differently according to companyâs role in the agro-food chain. Yet, there is need to exploit further the potential role of fairness practices in defining effective business strategies to contribute to higher equity and sustainability in the agro-food system
The study of a barley epigenetic regulator, HvDME, in seed development and under drought
Genomic and 16S metabarcoding data of Holothuria tubulosa Gmelin, 1791
Holothuria tubulosa Gmelin, 1791 is an edible sea cucumber species widespread in the Mediterranean Sea with ecological and increasing economic importance. Genome data of holothurian species is limited and the availability of genomic data resources is crucial in understanding their biology and adaptability mechanisms. This dataset presents the raw genome sequence data of H. tubulosa sequenced on an Illumina NextSeq 2000 platform. Genome size estimation was performed based on k-mer frequency approach. Additionally, the bacterial microbiome in the stomach and intestine of H. tubulosa collected from the Strymonian Gulf (North Aegean Sea, Greece) through 16S rRNA amplicon metabarcoding sequencing is reported. Sequencing was performed on an Illumina MiSeq platform. Analysis was conducted using the QIIME2 software package, the DADA2 algorithm and a trained classifier for taxonomy assignment. The datasets presented in this work serve as valuable resources for a comprehensive investigation of H. tubulosa at the genome level and for comparative genomics and echinoderms gut microbial studies
Molecular and Biochemical Characterization of the Greek Pepper (Capsicum annuum) Cultivars âFlorinisâ and âKaratzovaâ
Peppers are the fourth most important vegetable in the global food economy. Greek pepper cultivars âFlorinisâ and âKaratzovaâ are especially popular because of the signature red color and sweet taste of the fruits. The economic interest in âFlorinisâ peppers has led to many adulteration events. In that aspect, genetic profiles of âFlorinisâ, a âFlorinisâ-type and âKaratzovaâ peppers, were studied using Inter Simple Sequence Repeats (ISSR) molecular markers and an automated fragment detection system. Biochemical parameters, such as total dietary fiber, total phenolic and lycopene contents, and sugar profile that affect the fruit organoleptic and nutritional properties were evaluated. The molecular protocol established during this study may successfully discriminate the original âFlorinisâ cultivar from the âFlorinisâ-type peppers. âKaratzovaâ cultivar, which fruits are similar to âFlorinisâ, presented also a unique profile. The biochemical evaluation revealed that âFlorinisâ peppers had the highest sweetness index and total phenolic content. Such an analysis could be used for the discrimination of pepper cultivars sharing common morphological traits ensuring the unique identity of each cultivar and protecting farmers and consumers from fraud