Enhancing high-intensity actions during a basketball game after a strength training program with random recovery times between sets

To examine the effects of a strength training program with random recovery times between sets in consideration of several physical parameters, high-intensity actions (HIA), and spatial exploration index during a simulated basketball game. Twenty male basketball players (age: 19.45 ± 4.36 years) were assigned randomly, either to strength training group (n = 10), or a control group (n = 10). The strength training included: parallel back squat and bench press exercises, twice a week for the duration of 10 weeks, with two blocks of 5 sets × 5 repetitions interspersed with variable passive recovery (range = 15–35 sec.) between sets, and constant passive recovery (3-min) between blocks with the load that maximized propulsive power output. The pre- and post-test assessments included jumping (bilateral and unilateral), change-of-direction, straight sprinting, and a 5-on-5 full-court situation. The external training load was assessed using the local positioning system, and the internal load was recorded with the use of individual heart rate monitors. A significant interaction effect (group x time) was observed on countermovement jump (CMJ), unilateral right hops, high-intensity accelerations and decelerations, and peak accelerations and decelerations in the 5-on-5 full-court situation. Relative improvements observed and recorded in the training group on unilateral right hops, accelerations, and decelerations were correlated. Similar results were observed on 0–25m sprints, high-intensity decelerations, peak accelerations, and decelerations. Strength training paired with random recovery times enhanced physical and game-related aspects in the observed basketball players

Rotational flywheel training in youth female team sport athletes: could inter-repetition movement variability be beneficial?

Background: The aim of this study was to analyse the effects of an inter-repetition variable rotational flywheel training program (Variable) over standard rotational flywheel training (Standard). Methods: Twenty-four youth female team-sports players were randomly assigned to both training groups (Variable, n = 12; Standard, n = 12), which consisted of 1 set of 3 rotational flywheel exercises x 10-12 repetitions, biweekly for a period of 6-weeks. The participants included in Variable group were instructed to perform the movement randomly in one of the three directions (0º, 45º right, and 45º left). Measurements included reactive strength, jumping, change of direction, and sprinting tests; patellar tendon condition was also assessed. Results: Substantial improvements were found in vertical jump with left leg (16.9%), lateral jump with right leg (13.6%), and patellar condition in left leg (4.1%) for Standard group, but also in reactive strength index in right leg landing (33.9%), vertical jump with right (10.1%) and left leg (12.0%) for Variable group. A significant interaction effect (group x time) was observed on patellar condition in right leg (F = 10.02, p < 0.01, η 2 = 0.37), favoring Variable group. Conclusions: Rotational flywheel training programs were beneficial for youth-female team-sports athletes, although the movement variability may play a key role to develop different and specific physical adaptations

Explorando la distribución espacial colectiva en el baloncesto

Este estudio tuvo como objetivo analizar la distribución espacial de los jugadores de acuerdo con la eficacia de las acciones de juego de baloncesto. Tres partidos oficiales de baloncesto, campeonato portugués U14, fueron filmados y después analizados con el fin de seleccionar 10 secuencias de juego que terminaron en éxito ofensivo o defensivo. Las trayectorias de los jugadores en la cancha fueron digitalizadas con el fin de acceder a sus coordenadas reales con software TACTO. Este procedimiento ha permitido calcular el número de atacantes, defensores y ratio atacantes/defensores en cada uno de las siete áreas de la cancha (A1, A2, A3, A4, A5, A6, A7), sobre una base de momento a momento en toda la secuencia de juego. La distribución espacial de los jugadores ha ocurrido en función de la posición relativa a la canasta. En particular, la superioridad numérica defensiva en las zonas más cercanas a la canasta (A3) se relacionó con una mayor eficacia ofensiva. Estos resultados sugieren que la interacción entre constreñimientos, probablemente relacionados con la proximidad a la canasta, la ineficacia de las habilidades defensivas y la eficacia de la movilidad atacante puedenhaber desencadenado patrones colectivos distintivos de distribución espacial

Tree Nuts Anaphylaxis in Preschool Age Children

Summary: Introduction. The incidence of food-induced anaphylaxis (FIA) is increasing in young children. Although the commonest culprits are cow's milk and egg, FIA to tree nuts (TNs) have been increasing. Objective. Characterization of children referred to our allergy department due to TNs-induced anaphylaxis (TNs-FIA) during preschool age. Materials and methods. We have retrospectively included 25 children with clinical history of preschool TNs-FIA, proven by allergological work-up. TNs sensitization was assessed by skin prick tests and/or specific IgE. Results. The mean age of the first anaphylactic episode was 3.1±1.2 years. The majority (92%) had an allergic disease (52% asthma). The implicated TNs were cashew (11 children), walnut (8), pine nut (5), hazelnut (2) and almond (1). The reaction occurred after the first known ingestion in 68%. In 92%, symptoms appeared within 30 minutes after exposure. The most frequent clinical symptoms were mucocutaneous (96%), respiratory (80%) and gastrointestinal (52%). Twenty-one children were admitted to the emergency department, although only 48% were treated with epinephrine. An underneath IgE-mediated mechanism was proven in all cases. Immunologic cross-reactivity with other TNs was identified in 84%, and with peanut in 36%. Overall, in our center, TNs-FIA represents 18% of all causes of FIA. Conclusions. In preschool age children with TNs-FIA, cashew and walnut were the commonest implicated nuts. Most reactions occurred briefly after exposure to minimal amounts of TNs, demonstrating the high potency of these allergens. About one-third also had peanut sensitization. Potentially life-threatening TNs allergy can occur early in childhood and adequate management should be undertaken.publishersversionpublishe

Group versus Individualised Minimum Velocity Thresholds in the Prediction of Maximal Strength in Trained Female Athletes

This study examined the accuracy of di erent velocity-based methods in the prediction of bench press and squat one-repetition maximum (1RM) in female athletes. Seventeen trained females (age 17.8 1.3 years) performed an incremental loading test to 1RM on bench press and squat with the mean velocity being recorded. The 1RM was estimated from the load–velocity relationship using the multiple- (8 loads) and two-point (2 loads) methods and group and individual minimum velocity thresholds (MVT). No significant e ect of method, MVT or interaction was observed for the two exercises (p > 0.05). For bench press and squat, all prediction methods demonstrated very large to nearly perfect correlations with respect to the actual 1RM (r range = 0.76 to 0.97). The absolute error (range = 2.1 to 3.8 kg) for bench press demonstrated low errors that were independent of the method and MVT used. For squat, the favorable group MVT errors for the multiple- and two-point methods (absolute error = 7.8 and 9.7 kg, respectively) were greater than the individual MVT errors (absolute error = 4.9 and 6.3 kg, respectively). The 1RM can be accurately predicted from the load–velocity relationship in trained females, with the two-point method o ering a quick and less fatiguing alternative to the multiple-point method

Loss of Kat2a enhances transcriptional noise and depletes acute myeloid leukemia stem-like cells.

Acute Myeloid Leukemia (AML) is an aggressive hematological malignancy with abnormal progenitor self-renewal and defective white blood cell differentiation. Its pathogenesis comprises subversion of transcriptional regulation, through mutation and by hijacking normal chromatin regulation. Kat2a is a histone acetyltransferase central to promoter activity, that we recently associated with stability of pluripotency networks, and identified as a genetic vulnerability in AML. Through combined chromatin profiling and single-cell transcriptomics of a conditional knockout mouse, we demonstrate that Kat2a contributes to leukemia propagation through preservation of leukemia stem-like cells. Kat2a loss impacts transcription factor binding and reduces transcriptional burst frequency in a subset of gene promoters, generating enhanced variability of transcript levels. Destabilization of target programs shifts leukemia cell fate out of self-renewal into differentiation. We propose that control of transcriptional variability is central to leukemia stem-like cell propagation, and establish a paradigm exploitable in different tumors and distinct stages of cancer evolution

Modified Whole-Mount In situ Hybridization Protocol for the Detection of Transgene Expression in Electroporated Chick Embryos

hybridization. hybridization (WISH).Here we describe a modification to the WISH protocol that is essential to prevent DNA cross-hybridization and to specifically detect transgene mRNA transcripts in electroporated embryos. Our optimized WISH procedure can be applied not only to electroporated chick embryos but also to other embryos or adult tissues that have been transfected with large amounts of reporter- or expression construct DNA

Oxacillin sensitization of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius by antisense peptide nucleic acids in vitro

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.BACKGROUND: Antibiotic resistance genes can be targeted by antisense agents, which can reduce their expression and thus restore cellular susceptibility to existing antibiotics. Antisense inhibitors can be gene and pathogen specific, or designed to inhibit a group of bacteria having conserved sequences within resistance genes. Here, we aimed to develop antisense peptide nucleic acids (PNAs) that could be used to effectively restore susceptibility to β-lactams in methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP). RESULTS: Antisense PNAs specific for conserved regions of the mobilisable gene mecA, and the growth essential gene, ftsZ, were designed. Clinical MRSA and MRSP strains of high oxacillin resistance were treated with PNAs and assayed for reduction in colony forming units on oxacillin plates, reduction in target gene mRNA levels, and cell size. Anti-mecA PNA at 7.5 and 2.5 μM reduced mecA mRNA in MRSA and MRSP (p < 0.05). At these PNA concentrations, 66 % of MRSA and 92 % of MRSP cells were killed by oxacillin (p < 0.01). Anti-ftsZ PNA at 7.5 and 2.5 μM reduced ftsZ mRNA in MRSA and MRSP, respectively (p ≤ 0.05). At these PNA concentrations, 86 % of MRSA cells and 95 % of MRSP cells were killed by oxacillin (p < 0.05). Anti-ftsZ PNAs resulted in swelling of bacterial cells. Scrambled PNA controls did not affect MRSA but sensitized MRSP moderately to oxacillin without affecting mRNA levels. CONCLUSIONS: The antisense PNAs effects observed provide in vitro proof of concept that this approach can be used to reverse β-lactam resistance in staphylococci. Further studies are warranted as clinical treatment alternatives are needed.Peer reviewedFinal Published versio