Vegetative Propagation of PeatT Forest Tree Combretocarpus rotundatus (TUMIH (.Miq) Dancer) with Apical Cutting Methods

Cutting  is  plant  propagation  system which  relatively  easy  and  produces  seeds with good  quality with the similar characteristics as its parent and takes less time. Until now, the success rooted cuttings of tumih still not known certainly. The research aim is determine the effect of giving the hormone IBA 100 ppm, NAA 100 ppm and combination of IBA 50 ppm  and  NAA 50  ppm  on the growth  of  Tumih  shoots  cuttings  (Combretocarpus  rotundatus  (.Miq)  Danser).  The conclusion  that can be  taken on the basis of this research was the addition of plant growth regulators  (IBA, NAA, and IBA + NAA) has no significant effect on the growth of shoots cuttings Tumih (Combretocarpus rotundatus (.Miq) Danser) so  that  the  use  of  growth  regulators  is  not  needed  in  breeding  of  Tumih  shoots  cuttings  (Combretocarpus  rotundatus (. Miq) Danser)

Explant Sterilization and Shoot Induction Techniques in Micropropagation of Tembesu (Fagraea fragrans Roxb.) TEKNIK STERILISASI EKSPLAN DAN INDUKSI TUNAS DALAM MIKROPROPAGASI TEMBESU (Fagraea fragrans ROXB)

Tembesu (Fragraea fragans Roxb) is important tree species for furniture due to its decorative structure, but its propagation facing to the problems on seed availability. Determining explant sterilization technique of tembesu (Fagraea fragrans Roxb.) in in vitro condition is needed to produce aseptic explants for shoot induction in micropropagation techniques. Shoot induction of explants depends on plant growth regulator and growth medium compositions. This study was aimed (1)to analyzing the effect of sodium hypochlorite 10% in sterilization process to obtain aseptic explants, (2) to find out plant growth regulator and growth medium composition on shoot induction of tembesu (F. fragrans Roxb.) in in vitro condition. This study was conducted at Tissue Culture Laboratory, Rumpin Seed Source and Nursery Center, The Ministry of Forestry, Bogor on February – June 2014. Explants were sterilized using sodium hypochlorite (NaOCl 5.25%) at 10% of concentrations in various durations (5, 10, 15, and 20 minutes). Shoot induction was done using MS medium, MS modified medium, and MS added with 15% coconut water that combined with 1.5 ppm of BAP. The result showed that explants soaked in sodium hypochlorite 10% within 20 minutes was recommended for explants steriliziton technique of tembesu. Combination treatment between BAP 1.5 ppm and MS modified medium was the most favorable culture medium for shoot induction of tembesu.Keyword: Benzylaminopurine, Fagraea fragrans, micropropagation, Murashige-Skoog modified, sodium hypochlorit

The Effect of Cultivation Conditions on Sacha Inchi (Plukenetia volubilis L.) Seed Production and Oil Quality (Omega 3, 6, 9)

Sacha Inchi (Plukenetia volubilis Linneo, Euphorbiaceae) is known as a Peruvian seed containing a high level of unsaturated fatty acids, such as α-linolenic acid (Omega-3), linoleic acid (Omega-6), and oleic acid (Omega-9). These essential fatty acids are important functional foods due to their benefits for human health. Considering its benefits, it is necessary to find the right cultivation conditions to develop Sacha Inchi in Indonesia, where it has not been widely cultivated. This study aimed to determine the adaptability of Sacha Inchi to different cultivation conditions and the effects of these conditions on seed production and oil quality. Sacha Inchi plants were cultivated under three different cultivation conditions (open area, mixed cultures, and agroforestry) and the seeds were harvested monthly to determine the seed production and oil quality. The results showed that seed production in the open area was higher than in other conditions. The highest oil yield from monthly harvesting was found in mixed cultures. Interestingly, the level of Omega-3 content in the agroforestry condition was higher compared to other conditions. The level of Omega-6 and Omega-9 content in the open area was higher than in mixed cultures and agroforestry. However, the content of unsaturated fatty acids in the three different cultivation conditions was not significantly different, being 91.88% in the open area, 92.53% in mixed cultures, and 92.97% for agroforestry. In conclusion, the cultivation of Sacha Inchi under open areas is recommended due to its seed productivity which will affect the total oil production