Seed endophytic bacterial profiling from wheat varieties of contrasting heat sensitivity

Wheat yield can be limited by many biotic and abiotic factors. Heat stress at the grain filling stage is a factor that reduces wheat production tremendously. The potential role of endophytic microorganisms in mitigating plant stress through various biomolecules like enzymes and growth hormones and also by improving plant nutrition has led to a more in-depth exploration of the plant microbiome for such functions. Hence, we devised this study to investigate the abundance and diversity of wheat seed endophytic bacteria (WSEB) from heatS (heat susceptible, GW322) and heatT (heat tolerant, HD3298 and HD3271) varieties by culturable and unculturable approaches. The results evidenced that the culturable diversity was higher in the heatS variety than in the heatT variety and Bacillus was found to be dominant among the 10 different bacterial genera identified. Though the WSEB population was higher in the heatS variety, a greater number of isolates from the heatT variety showed tolerance to higher temperatures (up to 55°C) along with PGP activities such as indole acetic acid (IAA) production and nutrient acquisition. Additionally, the metagenomic analysis of seed microbiota unveiled higher bacterial diversity, with a predominance of the phyla Proteobacteria covering >50% of OTUs, followed by Firmicutes and Actinobacteria. There were considerable variations in the abundance and diversity between heat sensitivity contrasting varieties, where notably more thermophilic bacterial OTUs were observed in the heatT samples, which could be attributed to conferring tolerance against heat stress. Furthermore, exploring the functional characteristics of culturable and unculturable microbiomes would provide more comprehensive information on improving plant growth and productivity for sustainable agriculture

Patent Trends in ICAR institutes - A Review

260-265There is growing awareness amongst research institutes, though comparatively feeble and less competitive than the fierce corporate world, in filing and protecting their intellectual property rights. The article highlights statistics that will help identify and address patent protection matters and related issues. The current study provides a glimpse into the status of patent grants in the Indian Council of Agriculture Research (ICAR) institutes addressing all forms of plant and animal research and presents an overview. Areas of agro machinery, agrochemicals, food and dairy technology have the maximum number of innovations patented in the last decade with IARI being the top applicant and grantee

Patent trends in ICAR institutes- A Review

There is growing awareness amongst research institutes though comparatively feeble and less competitive than the fierce corporate in filing and protecting their IP rights. We aim to go through and highlight statistics that will help identify and address patent protection matters and related issues. The current study provides a glimpse into the status of patent grants in Indian Council of Agriculture Research (ICAR) institutes. The website of the Office of Controller General of Patents, Designs and trademarks was used to access the patents granted to all the ICAR institutes addressing all forms of plant and animal research and an overview is presented. Areas of agro machinery, agrochemicals, food and dairy technology have the maximum number of innovations patented in the last decade with IARI being the top applicant and grantee

Mitigating Drought Stress Effects in Arid and Semi-Arid Agro-Ecosystems through Bioirrigation Strategies—A Review

Drought stress is most alarming and destructive among the abiotic stresses that increased in intensity in recent years affecting global food and nutritional security. The main resource limiting global agricultural productivity is water. The previous two decades have seen a surprising amount of study reports on genetically modifying plants to increase their ability to withstand drought, but actual progress has lagged behind expectations. Applying bioirrigation techniques in drought-prone areas might be a workable alternative strategy. It does apply to the usage of living things or biological creatures, such as potential microorganisms that can move soil moisture from a zone with enough water to plants that are drought-stressed through the modification of agricultural microclimate using agronomic strategies. Potential microorganisms include Gram+ and Gram− bacterial consortiums, as well as plant-growth-promoting rhizobacteria (PGPR). In addition to PGPR, the utilization of soil macro-fauna in agriculture, such as earthworms, lugworms, termites, etc., can be utilized and explored in the near future towards bioirrigation. Earthworms and other macro-fauna are abundant in soil, digging deep burrows in the sediment and providing aeration to the plants. PGPR evolved with plant roots to enhance plant resilience under biotic and abiotic stresses. Benthic microorganisms, which include bacteria and microalgae, for instance, have a 70–80% higher water-holding capacity. Through research findings, these benthic microorganisms can be successfully identified and used in agriculture, and they may prove to be a cutting-edge method to increase plant-water-use efficiency. Similarly, plant roots of legume plants act as bioindicators under drought-stress conditions. These new developments make a significant contribution to addressing the problems of food security that come with changing climate. This review article offers information on bioirrigation techniques, their potential, estimating techniques, etc. Overall, this article goes into detail about how bioirrigation techniques aid crop plants in overcoming drought stress. Future research should focus on creating the most appropriate and effective microbes, dealing with the problem of delivery systems, and evaluating potential organisms in the field for microbial formulations to improve plant performance under drought stress and significantly lower yield losses in drought-affected areas

Nickel Bioremediation by Different Wetland Macrophytes Root Associated Bacteria

A study was conducted at ICAR-Indian Agricultural Research Institute, New Delhi (India) to enumerate bacterial load associated with five different macrophytes viz. Typha latifolia, Phragmites karka, Acorus calamus, Arundo donax and Vetiver zizanioides maintained after monthly applications of metal solution of 5.0 ppm of nickel. The roots of macrophytes supported bacterial associations amounting to 2.25×10 7 cfu/g and of them fifty two bacterial morphotypes were isolated. Mean nickel removal efficiency of the bacterial isolates was 11.06% after 3 days with maximum of 17.98% by the bacteria isolated from Phragmites. Most efficient thirteen dominant morphotypes for Ni-bio-accumulation were identified with 16S rRNA sequencing. Bacillus were the predominant bacterial genera (53.85%) followed by Pseudomonas and Microbacter (15.38%) which had shown the nickel removal capacity of 19.78%, 27.66% and 16.90% after 3 days of incubation, respectively. However, Ni removal by Pseudomonas mendocina isolated from Vacha root was maximum (34.20%). The overall results showed that macrophytes root associated bacteria may play an important role in plant assisted Nickel bio- remediation.ICA

Endophytic bacterial taxonomic and functional diversity in the seeds of wheat genotypes from different agroecologies

ABSTRACTPlant genotype and agroecology influence the composition and functionality of seed endophytic bacterial communities. Taxonomic analysis of 123 wheat seeds endophytic bacteria classified these into 23 genera predominantly under Firmicutes followed by Actinobacteria and Proteobacteria. Genus Bacillus was most abundant (30.7%) followed by Streptomyces (18.4%) with other representative genera such as Stenotrophomonas, Paenibacillus, Mixta, Enterobacter, Micrococcus, Pantoea, Alkalihalobacillus, Cortiobacterium, and more. Across agroecologies, the core microbiota of seeds consists of Bacillus, Streptomyces, Paenibacillus, and Stenotrophomonas, with maximum diversity and abundance observed in seeds of the North Western Plain Zone of India. Seed endophytic bacteria had PGP traits; nitrogen fixation (n = 101), production of IAA (n = 65), siderophores (n = 43), ammonia (n = 82) and solubilization of phosphate (n = 47), potassium (n = 37), and zinc (n = 8). The isolates produced HCN and hydrolytic enzymes and displayed antagonism against fungal pathogens. Overall, the information generated on wheat seeds’ endophytic bacterial taxonomy and beneficial traits may pave the path for the development of novel bioinoculants.Key message Wheat seeds from various agro-ecologies of India harbor diverse endophytic bacteria.Firmicutes were dominant followed by Actinobacteria and Proteobacteria.Genus Bacillus, Stenotrophomonas, Streptomyces, and Paenibacillus were core endophytic bacteria in different agro-ecologies.The endophytic bacterial strains were displaying diverse functional traits

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Not AvailableRhizosphere microbial communities are dynamic and play a crucial role in diverse biochemical processes and nutrient cycling. Soil type and cultivar modulate the composition of rhizosphere microbial communities. Changes in the community composition significantly alter microbial function and ecological process. We examined the influence of soil type on eubacterial and diazotrophic community abundance and microbial metabolic potential in chickpea (cv. BG 372 and cv. BG 256) rhizosphere. The total eubacterial and diazotrophic community as estimated through 16 S rDNA and nifH gene copy numbers using qPCR showed the soil type influence with clear rhizosphere effect on gene abundance. PLFA study has shown the variation in microbial community structure with different soil types. Differential influence of soil types and cultivar on the ratio of Gram positive to Gram negative bacteria was observed with most rhizosphere soils corresponding to higher ratios than bulk soil. The rhizosphere microbial activities (urease, dehydrogenase, alkaline phosphatase and beta-glucosidase) were also assessed as an indicator of microbial metabolic diversity. Principal component analysis and K-means non-hierarchical cluster mapping grouped soils into three categories, each having different soil enzyme activity or edaphic drivers. Soil type and cultivar influence on average substrate utilization pattern analyzed through community level physiological profiling (CLPP) was higher for rhizosphere soils than bulk soils. The soil nutrient studies revealed that both soil type and cultivar influenced the available N, P, K and organic carbon content of rhizosphere soil. Our study signifies that soil type and cultivar jointly influenced soil microbial community abundance and their metabolic potential in chickpea rhizosphere.Not Availabl

Phenetic and Molecular Diversity of Nitrogen Fixating Plant Growth Promoting Azotobacter Isolated from Semiarid Regions of India

In the present study, 24 Azotobacter strains were isolated from soils of different areas of southern Rajasthan and characterized at biochemical, functional, and molecular levels. The isolated Azotobacter strains were gram negative and cyst forming when viewed under the microscope. These strains were also screened for their plant growth promoting activities and the ability of these isolates to survive under abiotic stress conditions viz. salt, pH, temperature, and drought stress. All the isolates showed IAA, siderophore, HCN, and ammonia production, whereas seven Azotobacter strains showed phosphate solubilization. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed significant diversity among Azotobacter strains and the dendrogram obtained differentiated twenty-four of the strains into two major clusters at a similarity coefficient of 0.64. Qualitative and quantitative N2 fixation abilities of these strains were also detrained, and the amounts of acetylene reduced by Azotobacter strains were in the range of 1.31 to 846.56 nmol C2H4 mg protein−1 h−1. The strains showing high nitrogen fixation ability with multiple PGP activities were selected for further pot studies, and these Azotobacter strains significantly increased the various plant growth parameters of maize plantlets. Furthermore, the best Azotobacter isolates were subjected to 16S rRNA sequencing and confirmed their identities as Azotobacter sp. The indigenous Azotobacter strains with multiple PGP activities could be further used for commercial production

Two-step Growth of Alumina Nanoparticle Decorated Graphene Oxide Surfaces: Effect on Photocatalytic Activity

SEM images of 30 wt.% Al2O3 loaded GO and Photocatalytic degradation of Methylene blu

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Not AvailableIt is imperative to find suitable strategies to utilize the native soil phosphorus (P), as natural rock phosphate deposits are at a verge of depletion. We explored two such cost-effective and eco-friendly strategies for native soil P solubilization: silicon (Si)-rich agro-wastes (as Si source) and phosphate solubilizing microorganism (PSM). An incubation study was conducted in a sub-tropical Alfisol for 90 days at 25 °C under field capacity moisture. A factorial completely randomized design with 3 factors, namely: Si sources (three levels: sugarcane bagasse ash, rice husk ash, and corn cob ash), PSM (two levels: without PSM, and with PSM); and Si doses [three levels: no Si (Si0), 125 (Si125) and 250 (Si250) mg Si kg−1 soil] was followed. The PSM increased solution P and soluble Si level by ∼22.2 and 1.88%, respectively, over no PSM; whereas, Si125 and Si250 increased solution P by ∼60.4 and 77.1%, as well as soluble Si by ∼41.5 and 55.5%, respectively, over Si0. Also, interaction of PSM × Si doses was found significant (P<0.05). Activities of soil enzymes (dehydrogenase, acid phosphatase) and microbial biomass P also increased significantly both with PSM and Si application. Overall, PSM solubilized ∼4.18 mg kg−1 of inorganic P and mineralized ∼5.92 mg kg−1 of organic P; whereas, Si125 and Si250 solubilized ∼3.85 and 5.72 mg kg−1 of inorganic P, and mineralized ∼4.15 and 5.37 mg kg−1 of organic P, respectively. Path analysis revealed that inorganic P majorly contributed to total P solubilization; whereas, soluble and loosely bound, iron bound and aluminium bound P significantly influenced the inorganic P solubilization. Thus, utilization of such wastes as Si sources will not only complement the costly P fertilizers, but also address the waste disposal issue in a sustainable manner.Not Availabl