Risk factors of visceral leishmaniasis: a case control study in north-western Ethiopia

Background Visceral leishmaniasis (VL, also called “kala-azar”), is a life threatening neglected tropical infectious disease which mainly affects the poorest of the poor. VL is prevalent in Ethiopia particularly in the northwest of the country. Understanding the risk factors of VL infection helps in its prevention and control. The aim of the present study was to identify the factors associated with VL. Methods A case–control study was carried out during the period of January-July 2013 in northwest Ethiopia. Cases and controls were diagnosed using clinical presentation, the rk39 rapid diagnostic test and Direct Agglutination Test (DAT). A total of 283 (84.8% males versus 15.2% females) participants were interviewed. 90 cases and 193 controls were involved, matched by age, sex and geographical location with a ratio of 1:2 (case: controls). Univariate and backward multivariate conditional logistic regression were used to identify risk factors of VL. Results Elevated odds of VL was associated with goat ownership (OR = 6.4; 95%: confidence interval [Cl]: 1.5-28.4), living in houses with cracked wall (OR = 6.4; 95% Cl: 1.6-25.6), increased family size (OR = 1.3; 95% Cl: 1.0-1.8) and the number of days spent in the farm field (OR = 1.1; 95% Cl: 1.0-1.2). However, daily individual activities around the home and farm fields, mainly sleeping on a bed (OR = 0.2; 95%: Cl 0.03-0.9), sleeping outside the house under a bed net (OR = 0.1; 95% Cl: 0.02-0.36)] and smoking plant parts in the house during the night time (OR = 0.1; 95% Cl: 0.01-0.6) were associated with decreased odds of being VL case. Conclusion Our findings showed that use of bed net and smoke could be helpful for the prevention of VL in the area particularly among individuals who spend most of their time in the farm. VL control effort could be focused on improving housing conditions, such as sealing cracks and crevices inside and outside houses. Further research is warranted to elucidate the role of goats in the transmission of L. donovani, assess the impact of bed nets and the role of the traditional practice of smoking plants

Effect of Acacia tortilis pods on intake, digestibility and nutritive quality of goat diets in southwestern Eritrea

Fresh Acacia tortilis pods were mixed with low quality native grass hay to form the following five rations: 100% hay (T1), 25% pods and 75% hay (T2), 50% pods and 50% hay (T3), 75% pods and 25% hay (T4) and 100% pods (T5) on ‘as fed' weight basis. Fifteen male Barka goats, approximately one year old and 10–15kg body weight, were randomly assigned to the five rations and fed in individual pens. Chemical composition, dry matter intake (DMI), in vitro dry and organic matter digestibility and body weight changes of the animals were evaluated. The average crude protein content of the pods was about 47% higher than the 7% minimum required for normal rumen function, while that of the hay was about 13% below. Percent ash, neutral detergent fibre (NDF), acid detergent fibre (ADF), hemi-cellulose (HC), cellulose (CL) and acid detergent lignin (ADL) contents were higher in the hay than in the pods. The pods were, however, generally higher in Ca, P, Mg and Na than the hay. Average DMI (g d-1 and g kg-1 LBW), percent in vitro dry and organic matter digestibility and body weight gains (total kg and gd-1) increased with an increase in A. tortilis pods, up to 75% level and then begun to decline. However, despite the decrease, T5 still had significantly (P < 0.05) higher feed digestibility and body weight gains than T2 and T3. Thus, supplementing low quality range herbage with Acacia pods can considerably improve the nutritive value of range livestock diets, particularly during the dry season when other types of fodder are of extremely low quality. Keywords: Acacia pods, Barka goat diet, range forage, pastoralists, semi-arid EritreaAfrican Journal of Range & Forage Science 2003, 20(1): 59–6

Summary of collected lesions and molecular typed AFB isolated from cattle slaughtered at six Ethiopian abattoirs.

*<p>NTM = Typed as <i>Mycobacterium</i> species not from the <i>M. tuberculosis</i> complex.</p

Oligonucleotide primers used for molecular typing of <i>Mycobacterium</i> isolates and sizes of the expected PCR products.

#<p>Primer set used for partial sequencing of the 16S rRNA locus <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005068#pone.0005068-Han1" target="_blank">[10]</a>.</p><p>NA, Not Applicable.</p

Spoligotyping patterns of A) <i>M. bovis</i> strains and B) <i>M. tuberculosis</i> strains isolated from cattle in Ethiopia.

*<p>SB No = <a href="http://www.mbovis.org" target="_blank">www.mbovis.org</a>; SIT No = Spoligo-International-Typing.</p

Non-tuberculous mycobacteria isolated from cattle in Ethiopia.

<p>Non-tuberculous mycobacteria isolated from cattle in Ethiopia.</p

Geographical distribution of <i>Mycobacterium</i> isolates from cattle in Ethiopia.

<p>The total number of <i>M. bovis</i> (•), <i>M. tuberculosis</i> (▪), and NTM (○), isolated from respective abattoir are indicated in respective box, as well as characterised spoligotype patterns. Approximate area coverage for each abattoir is shown by a solid circle.</p

The burden of mycobacterial disease in Ethiopian cattle:Implications for public health

BACKGROUND: Bovine tuberculosis (bTB), caused by Mycobacterium bovis, is a debilitating disease of cattle. Ethiopia has one of the largest cattle populations in the world, with an economy highly dependent on its livestock. Furthermore, Ethiopia has one of the highest incidence rates of human extrapulmonary TB in the world, a clinical presentation that is often associated with transmission of M. bovis from cattle to humans. METHODOLOGY/PRINCIPAL FINDINGS: Here we present a comprehensive investigation of the prevalence of bTB in Ethiopia based on cases identified at slaughterhouses. Out of approximately 32,800 inspected cattle, ∼4.7% showed suspect tuberculous lesions. Culture of suspect lesions yielded acid-fast bacilli in ∼11% of cases, with M. bovis accounting for 58 of 171 acid-fast cultures, while 53 isolates were non-tuberculous mycobacteria. Strikingly, M. tuberculosis was isolated from eight cattle, an unusual finding that suggests human to animal transmission. CONCLUSIONS/SIGNIFICANCE: Our analysis has revealed that bTB is widely spread throughout Ethiopia, albeit at a low prevalence, and provides underpinning evidence for public health policy formulation