Use of grayscale histogram to assess testicular changes in ram caused by heat stress

Because it detects discrete changes in tissue density, ultrasound allows an early diagnosis of physiologic changes, especially when dealing with subclinical changes. aimed to evaluate the use of quantitative analysis of the gray scale of ultrasound images of ovine testicles as a method for early diagnosis of testicular changes caused by heat stress. Testicles from ten ovine were insulated and then evaluated regarding echogenicity, echotexture, anatomical measurements, and seminal characteristics. Echogenicity was the first variable to show changes during the insulation period, as well as the first one to show evidence of regeneration of those changes. There was a correlation (p 0.05) in echotexture. Testicular measurements were only altered (p < 0.05) after insulation; however, the structural integrity of the seminiferous tubules was not altered. Echogenicity is an efficient ultrasound parameter for the early diagnosis of a testicular degenerative process as well as for the early diagnosis of its regeneration

Use of grayscale histogram to assess testicular changes in ram caused by heat stress

Because it detects discrete changes in tissue density, ultrasound allows an early diagnosis of physiologic changes, especially when dealing with subclinical changes. aimed to evaluate the use of quantitative analysis of the gray scale of ultrasound images of ovine testicles as a method for early diagnosis of testicular changes caused by heat stress. Testicles from ten ovine were insulated and then evaluated regarding echogenicity, echotexture, anatomical measurements, and seminal characteristics. Echogenicity was the first variable to show changes during the insulation period, as well as the first one to show evidence of regeneration of those changes. There was a correlation (p &lt; 0.05) between echogenicity and sperm vigor, motility, and mass sperm motility, as well as a correlation (p &lt; 0.05) between echotexture and motility. Echogenicity, motility, vigor, and mass sperm motility decreased (p &lt; 0.05) within four days of testicular insulation, and there was no change (p &gt; 0.05) in echotexture. Testicular measurements were only altered (p &lt; 0.05) after insulation; however, the structural integrity of the seminiferous tubules was not altered. Echogenicity is an efficient ultrasound parameter for the early diagnosis of a testicular degenerative process as well as for the early diagnosis of its regeneration.Because it detects discrete changes in tissue density, ultrasound allows an early diagnosis of physiologic changes, especially when dealing with sub-clinical changes. The study aimed to evaluate the use of quantitative analysis of the grayscale of ultrasound images of ovine testicles as a method for early diagnosis of testicular changes caused by heat stress. Testicles from ten rams were insulated and then evaluated regarding echogenicity, echotexture, anatomical measurements, and seminal characteristics. Echogenicity was the first variable to show changes during the insulation period, as well as the first one to show evidence of regeneration of those changes. There was a correlation (p &lt; 0.05) between echogenicity and sperm vigor, sperm motility, and mass sperm motility, as well as a correlation (p &lt; 0.05) between echotexture and motility. Echogenicity, sperm motility, vigor, and mass sperm motility decreased (p &lt; 0.05) within four days of testicular insulation, and there was no change (p &gt; 0.05) in echotexture. Testicular measurements were only altered (p &lt; 0.05) after insulation. Echogenicity is an efficient ultrasound parameter for the early diagnosis of a testicular degenerative process as well as for the early diagnosis of its regeneration

Ação ovicida do extrato bruto enzimático do fungo Pochonia chlamydosporia sobre ovos de Ancylostoma sp

Ancylostoma sp é um geo-helminto potencialmente zoonótico. O objetivo deste trabalho foi avaliar in vitro a ação do extrato bruto enzimático de Pochonia chlamydosporia (VC4) sobre ovos de Ancylostoma sp, em meio ágar-água 2% e em cultura de fezes. Observou-se um percentual de redução na eclosão dos ovos de Ancylostoma sp, de 76,8% na placas de Petri do grupo tratado em relação ao grupo controle. O extrato bruto enzimático de Pochonia chlamydosporia foi eficiente na redução da eclosão dos ovos de Ancylostoma sp, podendo ser utilizado como controlador biológico desse nematoide.Ancylostoma sp is a potentially zoonotic geohelminth. This study aimed to evaluate in vitro the action of crude enzyme extract of Pochonia chlamydosporia (VC4) on eggs of Ancylostoma sp in 2% water-agar and in fecal cultures. The percentage reduction in Ancylostoma sp egg eclosion was 76.8% in Petri dishes of the treated group compared to the control group. The crude enzyme extract of Pochonia chlamydosporia was effective at reducing Ancylostoma sp egg eclosion and can be used as biological control of this nematode

Normas e Recomendações para o Emprego do BCG em Câncer

Normas e recomendações da Divisão Nacional de Câncer – Ministério da Saúde, para o emprego do BCG em câncer

Identification of Eschweilenol C in derivative of Terminalia fagifolia Mart. and green synthesis of bioactive and biocompatible silver nanoparticles

A green synthetic route was developed to prepare silver nanoparticles (AgNPs) in aqueous solution for biological applications. Eschweilenol C, a compound derivative ellagic acid was identified as the main constituent of the aqueous fraction of the ethanolic extract of Terminalia fagifolia Mart. by NMR analysis. In the green synthesis, the ethanolic extract of T. fagifolia and its aqueous fraction were used to promote silver reduction and nanoparticle stabilization. The synthesized AgNPs presented a spherical or polygonal morphology shape by TEM analysis and AgNPs showed high levels of antioxidant and considerable antibacterial and antifungal activities. Synthesized nanoparticles presented significant antioxidant activity by sequestration of DPPH and ABTS radicals, in addition to iron reduction (FRAP assay) and measurement of antioxidant capacity in ORAC units, in addition, AgNP synthesized with the aqueous fraction also demonstrated antioxidant potential in microglial cells. Gram-positive and Gram-negative bacteria were susceptible to growth inhibition by the nanoparticles, among which the AgNPs formed by the ethanolic extract was the most effective. The data obtained by AFM images suggested that AgNPs could lead to the lysis of bacteria and subsequent death. The antifungal assays showed high efficiency against yeasts and dermatophytes. This work represents the first description of antifungal activity by AgNPs against Fonsecaea pedrosoi, the etiologic agent of chromoblastomycosis. In relation to biocompatibility, the AgNPs induced lower haemolysis than AgNO3.We thank Herbert Kogler and Reinhard Wimmer for the identification of Eschweilenol C. The NMR laboratory at Aalborg University is supported by the Obel Family, SparNord and Carlsberg foundations.The authors are grateful to Carla Eiras (LIMAV/CT/UFPI) and to FCT and EU for financial support through project UID/QUI/50006/2013– POCI-01-0145-FEDER-007265 from COMPETE and projectNORTE-01-0145-FEDER-000011 from COMPETE. Thanks to Andreia Pinto for help with the TEM measurements at Instituto de Medicina Molecular (IMM). This work was supported by the Histology and Comparative Pathology Laboratory of the IMMinfo:eu-repo/semantics/publishedVersio