Biopolimer-fém komplex rendszerek II.

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Effects of crude oil and oil fractions on the liver P450-dependent monooxygenase activities and antioxidant defence system of different freshwater fish species

The effects of crude oil (Szeged-Algyo, Hungary) and oil fractions (F1: rich in aromatics; F2 fraction: free from aromatics) were investigated on liver CYP1A isoenzymes and antioxidant defence system following their i.p. injection into different freshwater fish species: carp (Cyprinus carpio L.), silver carp (Hyphothalmichtys molitrix V.), and European eel (Anquilla anquilla). A dose of 2 mL kg(-1) crude oil enhanced EROD activity 8-fold in carp and only 5-fold in eel after 3 days. Oil fraction F1 caused only a 2-fold induction in EROD activity only in carp, while F2 fraction caused significant inhibition in all three investigated fish species. The antioxidant parameters [ lipid peroxidation (LP), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione (GSH)] were measured following the treatment. A decrease of 50% in CAT activity was observed after oil treatment. The GSH level enhanced, resulting the protective effects against LP. The same dose of crude oil but a longer duration time resulted in lower CYP1A induction in carp and antioxidant parameters had returned close to control. In all treatments the EROD isoenzymes proved to be more sensitive and the effects of oil treatment showed species to be different. Carp proved to be more sensitive than eel or silver carp

Expression of two metallothionein genes in different brain regions of common carp

The expression pattern of two metallothionein (MT) genes in response to temperature shock and exposure to Cd2+ was investigated in the brain of common carp (Cyprinus carpio), in whole-animal experiments. The changes in the levels of MT-1 and MT-2 mRNA in the olfactory lobe, midbrain and cerebellum were followed by semiquantitative RT-PCR. The inducibility of the two MT genes was brain regionand stressor-specific. Cd2+ affected mostly the expression of MT-2, while the level of the MT-1 transcript did not change significantly in any of the brain regions examined. Moreover, the MT-2 expression was regulated spatially; MT-2 was induced significantly more strongly in the olfactory lobe than in the cerebellum or midbrain. A sudden temperature drop mainly affected the expression of the MT-1 gene; after 5 h of cold shock, the MT-1 mRNA level was about 25% of the basal value in the cerebellum and the midbrain region. The MT-2 expression did not change significantly during this treatment

Effects of Cu2+ and Pb2+ on different fish species: Liver cytochrome P450-dependent monooxygenase activities and FTIR spectra

The effects of Cu2+-sulfate and Pb2+-acetate on carp (Cyprinus carpio L.), silver carp (Hypopthalmichtys molitnix V.) and wels (Silurus glanis L.) were studied. The liver microsomal Cyt P450 content, the EROD, ECOD and APND monooxygenase activities were measured. In vivo treatment with 1 mg L-1 Cu2+ significantly elevated the activities of these enzymes and Cyt P450 content in silver carp livers. The high-dose Cu2+ treatment (10 mg L-1) on silver carp caused two-fold higher induction in the P450 dependent monooxygenase isoensymes than in wels. Although the 2 mg kg 1 treatment with Pb2+ in carp elevated significantly the P450 content, the EROD isoenzyme activities were significantly decreased after 1 day, showing the destructive effect of metal ion on the enzyme system. In vitro, Cu2+ and Pb2+ decreased the Cyt P450 content in the carp liver microsomes and the absorption peak shifted to higher wavelength. Fourier Transform Infrared (FTIR) spectroscopy was used to detect the damaging effects of the heavy metals. According to the inhibitory potency to Cu2+, the most sensitive isoenzyme was the EROD in wels, the least was the silver carp's isoenzyme. The investigated fish P450 isoenzymes showed, that the Cu2+ was a stronger inhibitor than Pb2+

Effects of crude oil and oil fractions on the liver P450-dependent monooxygenase activities and antioxidant defence system of different freshwater fish species

The effects of crude oil (Szeged-Algyő, Hungary) and oil fractions (F1: rich in aromatics; F2 fraction: free from aromatics) were investigated on liver CYP1A isoenzymes and antioxidant defence system following their i.p. injection into different freshwater fish species: carp ( Cyprinus carpio L.), silver carp ( Hyphothalmichtys molitrix V.), and European eel ( Anquilla anquilla ). A dose of 2 mL kg −1 crude oil enhanced EROD activity 8-fold in carp and only 5-fold in eel after 3 days. Oil fraction F1 caused only a 2-fold induction in EROD activity only in carp, while F2 fraction caused significant inhibition in all three investigated fish species. The antioxidant parameters [lipid peroxidation (LP), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione (GSH)] were measured following the treatment. A decrease of 50% in CAT activity was observed after oil treatment. The GSH level enhanced, resulting the protective effects against LP. The same dose of crude oil but a longer duration time resulted in lower CYP1A induction in carp and antioxidant parameters had returned close to control. In all treatments the EROD isoenzymes proved to be more sensitive and the effects of oil treatment showed species to be different. Carp proved to be more sensitive than eel or silver carp