Anti Quorum Sensing Activity of Kayu Manis Leaves Extracts (Cinnamomun Burmannii Ness. Ex Bl.) Against Pseudomonas Aeruginosa

Quorum sensing is a communication system among bacterial cells which correlates with biofilm formation. Biofilm can protect bacteria from environment including antibiotic of which can cause higher antibiotic concentration of 100 up to 1000 times. Inhibition of quorum sensing is expected to inhibit the biofilm formation. The cinnamon bark (Cinnamomum burmanii Ness. Ex Bl.) has been known to have antibacterial and antibiofilm activities. Leaves are available abundantly which urges a research to find out the activity as anti quorum sensing against Pseudomonas aeruginosa. The succesive maceration of dried pulverized leaves produced hexane, ethyl acetate, and methanol extracts. Antibacterial activity was observed by microdillution method with MTT assay. Afterwards, the active extract was examined for anti quorum sensing activity by diffusion method in cetrimide Agar. Quorum sensing activity was shown by dark zone (opaque) growth around sample application, observed under UV light of 366 nm. TLC bioautography method was done to observe the active spots by using silica gel F254 as the stationary phase, chloroform-methanol (6:1 v/v) as the mobile phase, loading sample used was 1.25 mg and 30 min of plate contact duration. The ethyl acetate extract inhibited growth of P. aeruginosa with shown by MIC at 8 µg/µL Quorum sensing as well as growth inhibition activities were observed at loading samples 12,5 and 25 mg/wells, while at 6,25 mg the extract only exhibited quorum sensing inhibition. Presences of substances having phenolic, flavonoid, alkaloid and aldehyde/keton as functional groups were detected by TLC method of the extract but no active spot identified on bioautography

Peningkatan Pemahaman Penggunaan Vitamin dan Long Covid di Kota Mataram dan Lombok Barat

Pemerintah Kota Mataram dan Lombok Barat mengkonfirmasi jumlah kasus sembuh covid per 19 September 2021 6599 kasus dan 3113 kasus. Berdasarkan data tersebut akan ada sekitar enam ribuan orang di Kota Mataram dan tiga ribuan orang di Lombok Barat bisa mengalami gejala long covid-19. Minimnya pengetahuan mengenai penggunaan vitamin, long covid dan membantu program pemerintah untuk memberikan perhatian khusus pada pasien yang mengalami gejala long covid menjadi alasan dilakukan pengabdian ini. Tujuan pengabdian ini adalah memberikan pemahaman masyarakat mengenai long covid dan penggunaan vitamin pada masa pandemi covid-19. Metode pelaksanaan kegiatan ini adalah a) edukasi secara concurrent b) dialog interaktif c) diberikan pendampingan langsung memilih vitamin yang sesuai kebutuhan di Kota Mataram dan Lombok Barat yang dilakukan pada 29 - 30 Oktober 2021. Evaluasi kegiatan ini dilakukan dengan dialog interaktif dengan masyarakat secara singkat. Hasil kegiatan ini adalah telah berhasil meningkatkan pengetahuan masyarakat terhadap long covid dan penggunaan vitamin. Hal ini terlihat dari antusiasi masyarakat dengan diskusi singkat