29 research outputs found
Time-domain THz spectroscopy reveals coupled protein-hydration dielectric response in solutions of native and fibrils of human lyso-zyme
Here we reveal details of the interaction between human lysozyme proteins,
both native and fibrils, and their water environment by intense terahertz time
domain spectroscopy. With the aid of a rigorous dielectric model, we determine
the amplitude and phase of the oscillating dipole induced by the THz field in
the volume containing the protein and its hydration water. At low
concentrations, the amplitude of this induced dipolar response decreases with
increasing concentration. Beyond a certain threshold, marking the onset of the
interactions between the extended hydration shells, the amplitude remains fixed
but the phase of the induced dipolar response, which is initially in phase with
the applied THz field, begins to change. The changes observed in the THz
response reveal protein-protein interactions me-diated by extended hydration
layers, which may control fibril formation and may have an important role in
chemical recognition phenomena
Spectral simplification techniques for high resolution fourier transform spectroscopic studies
Abstract not availabl
Spectral simplification techniques for high resolution fourier transform spectroscopic studies
Abstract not availabl
FOURIER TRANSFORM INFRARED EMISSION SPECTROSCOPY OF GAS-PHASE MnH, MnD AND YbH
Author Institution: Department of Physics, University of Waterloo; Guelph-Waterloo Centre for Graduate Work in Chemistry and Biochemistry, University of WaterlooMolecules in the gas phase were generated in an emission source that combines a high temperature tube furnace with an electrical discharge. Infrared emission spectra of MnH/D in its ground state were recorded using a Fourier transform spectrometer at a resolution of . The vibrational bands to for MnH and to for MnD were observed. The vibration-rotation emission spectrum of YbH in its ground state was recorded at a resolution of . Lines corresponding to five isotopomers of Yb were recorded for the band. In addition some weak electronic transitions of YbH/D in the near-infrared were observed and their analyses may be presented at the conference