S-TLLR: STDP-inspired Temporal Local Learning Rule for Spiking Neural Networks

Spiking Neural Networks (SNNs) are biologically plausible models that have been identified as potentially apt for the deployment for energy-efficient intelligence at the edge, particularly for sequential learning tasks. However, training of SNNs poses a significant challenge due to the necessity for precise temporal and spatial credit assignment. Back-propagation through time (BPTT) algorithm, whilst being the most widely used method for addressing these issues, incurs a high computational cost due to its temporal dependency. Moreover, BPTT and its approximations solely utilize causal information derived from the spiking activity to compute the synaptic updates, thus neglecting non-causal relationships. In this work, we propose S-TLLR, a novel three-factor temporal local learning rule inspired by the Spike-Timing Dependent Plasticity (STDP) mechanism, aimed at training SNNs on event-based learning tasks. S-TLLR considers both causal and non-causal relationships between pre and post-synaptic activities, achieving performance comparable to BPTT and enhancing performance relative to methods using only causal information. Furthermore, S-TLLR has low memory and time complexity, which is independent of the number of time steps, rendering it suitable for online learning on low-power devices. To demonstrate the scalability of our proposed method, we have conducted extensive evaluations on event-based datasets spanning a wide range of applications, such as image and gesture recognition, audio classification, and optical flow estimation. In all the experiments, S-TLLR achieved high accuracy with a reduction in the number of computations between $1.1-10\times$.Comment: 11 pages, 5 figure

Hardware/Software co-design with ADC-Less In-memory Computing Hardware for Spiking Neural Networks

Spiking Neural Networks (SNNs) are bio-plausible models that hold great potential for realizing energy-efficient implementations of sequential tasks on resource-constrained edge devices. However, commercial edge platforms based on standard GPUs are not optimized to deploy SNNs, resulting in high energy and latency. While analog In-Memory Computing (IMC) platforms can serve as energy-efficient inference engines, they are accursed by the immense energy, latency, and area requirements of high-precision ADCs (HP-ADC), overshadowing the benefits of in-memory computations. We propose a hardware/software co-design methodology to deploy SNNs into an ADC-Less IMC architecture using sense-amplifiers as 1-bit ADCs replacing conventional HP-ADCs and alleviating the above issues. Our proposed framework incurs minimal accuracy degradation by performing hardware-aware training and is able to scale beyond simple image classification tasks to more complex sequential regression tasks. Experiments on complex tasks of optical flow estimation and gesture recognition show that progressively increasing the hardware awareness during SNN training allows the model to adapt and learn the errors due to the non-idealities associated with ADC-Less IMC. Also, the proposed ADC-Less IMC offers significant energy and latency improvements, $2-7\times$ and $8.9-24.6\times$, respectively, depending on the SNN model and the workload, compared to HP-ADC IMC.Comment: 12 pages, 13 figure

Adiabatic Formation of Rydberg Crystals with Chirped Laser Pulses

Ultracold atomic gases have been used extensively in recent years to realize textbook examples of condensed matter phenomena. Recently, phase transitions to ordered structures have been predicted for gases of highly excited, 'frozen' Rydberg atoms. Such Rydberg crystals are a model for dilute metallic solids with tunable lattice parameters, and provide access to a wide variety of fundamental phenomena. We investigate theoretically how such structures can be created in four distinct cold atomic systems, by using tailored laser-excitation in the presence of strong Rydberg-Rydberg interactions. We study in detail the experimental requirements and limitations for these systems, and characterize the basic properties of small crystalline Rydberg structures in one, two and three dimensions.Comment: 23 pages, 10 figures, MPIPKS-ITAMP Tandem Workshop, Cold Rydberg Gases and Ultracold Plasmas (CRYP10), Sept. 6-17, 201

Fabrication par imprégnation directe et assemblage par soudage de composites thermoplastiques structuraux à renfort textile en fibres végétales

National audience* Développement d’une technique originale d'imprégnation directe de renforts continus de lin par des thermoplastiques totalement ou partiellement bio-sourcés, par injection dans un moule fermé* Étude multi-échelles des mécanismes d'imprégnation / gonflement sous contraintes thermo-hydromécaniques des fibres végétales. Déduction des évolutions de propriétés physiques et mécaniques* Étude des mécanismes d’imprégnation de composites moulés à haute cadence avec des thermoplastiques mono- ou bi-composants de haute fluidité* Assemblage de pièces composites renforcées de fibres végétales par soudage laser* Qualification de la résistance des joints de soudure des composites thermoplastiques bio-sourcé

Modulation of RANTES expression by HCV core protein in liver derived cell lines

<p>Abstract</p> <p>Background</p> <p>Hepatitis C virus (HCV) infection is associated with high percentage of chronicity which implies the ability of the virus to evade or modulate host cell immune system. Modulation of chemokines, such as RANTES may be part of the virus induced pathogenicity. We examined the effect of core and structural proteins of HCV on RANTES expression in two liver derived cell lines, HepG2 and Chang Liver (CHL).</p> <p>Methods</p> <p>HepG2 and Chang Liver (CHL) cell lines were established and selected for constitutive expression of HCV core and structural genes. Flow cytometry and quantitative RT-PCR analysis were performed to examine the effect of HCV core protein on RANTES expression. Luciferase analysis after RANTES-Luc-promoter transfection of established cell lines was assayed by luminometer measurements (RLU) of RANTES promoter activity. IRF-1 and IRF-7 expression was then examined by immunoblotting analysis.</p> <p>Results</p> <p>Results of flow cytometry and RT-PCR analysis indicated that RANTES is differentially regulated by HCV core protein in the two cell lines examined as its expression was inhibited in HepG2 cells, by a reduction of RANTES promoter activity. Conversely, RANTES protein and mRNA were induced by the core protein in CHL cells, through the induction of the promoter.</p> <p>Since HCV genome modulates IRF-1 and IRF-7 in replicon system and IRF-1, IRF-3 and IRF-7 have been reported to regulate RANTES promoter in various cell systems, analysis of the mechanism underlying RANTES modulation by the core protein revealed that IRF-1 expression was induced in HepG2 cells by the core protein, whereas in CHL cells it was expressed at a very low level that was not influenced by transfection with the core protein construct. This suggested that IRF-1 level may mediate the expression of RANTES in cell lines of liver origin. The effect of the core protein on RANTES promoter was countered by co-transfection with NF90, a double-stranded-RNA binding protein that activates some interferon response genes and acts as a component of cell defense against viral infection.</p> <p>Conclusion</p> <p>HCV core protein have opposite effects on the expression of RANTES in different cell types <it>in vitro</it>, possibly reflecting a similar scenario in different microenvironments <it>in vivo</it>.</p

Application of GFAT as a Novel Selection Marker to Mediate Gene Expression

The enzyme glutamine: fructose-6-phosphate aminotransferase (GFAT), also known as glucosamine synthase (GlmS), catalyzes the formation of glucosamine-6-phosphate from fructose-6-phosphate and is the first and rate-limiting enzyme of the hexosamine biosynthetic pathway. For the first time, the GFAT gene was proven to possess a function as an effective selection marker for genetically modified (GM) microorganisms. This was shown by construction and analysis of two GFAT deficient strains, E. coli ΔglmS and S. pombe Δgfa1, and the ability of the GFAT encoding gene to mediate plasmid selection. The gfa1 gene of the fission yeast Schizosaccharomyces pombe was deleted by KanMX6-mediated gene disruption and the Cre-loxP marker removal system, and the glmS gene of Escherichia coli was deleted by using λ-Red mediated recombinase system. Both E. coli ΔglmS and S. pombe Δgfa1 could not grow normally in the media without addition of glucosamine. However, the deficiency was complemented by transforming the plasmids that expressed GFAT genes. The xylanase encoding gene, xynA2 from Thermomyces lanuginosus was successfully expressed and secreted by using GFAT as selection marker in S. pombe. Optimal glucosamine concentration for E. coli ΔglmS and S. pombe Δgfa1 growth was determined respectively. These findings provide an effective technique for the construction of GM bacteria without an antibiotic resistant marker, and the construction of GM yeasts to be applied to complex media

Copper-Dependent Trafficking of the Ctr4-Ctr5 Copper Transporting Complex

In Schizosaccharomyces pombe, copper uptake is carried out by a heteromeric complex formed by the Ctr4 and Ctr5 proteins. Copper-induced differential subcellular localization may play a critical role with respect to fine tuning the number of Ctr4 and Ctr5 molecules at the cell surface.We have developed a bimolecular fluorescence complementation (BiFC) assay to analyze protein-protein interactions in vivo in S. pombe. The assay is based on the observation that N- and C-terminal subfragments of the Venus fluorescent protein can reconstitute a functional fluorophore only when they are brought into tight contact. Wild-type copies of the ctr4(+) and ctr5(+) genes were inserted downstream of and in-frame with the nonfluorescent C-terminal (VC) and N-terminal (VN) coding fragments of Venus, respectively. Co-expression of Ctr4-VC and Ctr5-VN fusion proteins allowed their detection at the plasma membrane of copper-limited cells. Similarly, cells co-expressing Ctr4-VN and Ctr4-VC in the presence of Ctr5-Myc(12) displayed a fluorescence signal at the plasma membrane. In contrast, Ctr5-VN and Ctr5-VC co-expressed in the presence of Ctr4-Flag(2) failed to be visualized at the plasma membrane, suggesting a requirement for a combination of two Ctr4 molecules with one Ctr5 molecule. We found that plasma membrane-located Ctr4-VC-Ctr5-VN fluorescent complexes were internalized when the cells were exposed to high levels of copper. The copper-induced internalization of Ctr4-VC-Ctr5-VN complexes was not dependent on de novo protein synthesis. When cells were transferred back from high to low copper levels, there was reappearance of the BiFC fluorescent signal at the plasma membrane.These findings reveal a copper-dependent internalization and recycling of the heteromeric Ctr4-Ctr5 complex as a function of copper availability