Study of ecto and endo parasites detected in barbers in Kırıkkale city

Bağırsak parazitleri ülkeler arasında olduğu gibi bölgeler hatta iller arasında da değişebilmekte ve insanların beslenme alışkanlıkları da bu farklılıklarda rol oynamaktadır. Berberler parazitlerin yayılmasında aracı rol oynayabilirler. Kırıkkale ilindeki 137 berberin parazit taşıma oranları hakkında bilgi edinmek amacıyla dışkı örnekleri toplanmış, makroskobik ve mikroskobik olarak incelenmiştir. Ayrıca berberlerden parazitolojik yönden şikayetleri ile ilgili anamnez alınmıştır. Mikroskobik incelemede nativ-lügol yöntemi uygulanmıştır. Nisan - Haziran 2000 tarihleri arasında yapılan prospektif incelemeler sonucunda, Kırıkkale ilindeki 17-65 yaşları arasındaki 137 erkek berberin saç ve ellerinde herhangi bir parazite rastlanmamıştır, 15'inde (%11) bağırsak paraziti tespit edilmiştir. Bulunan parazitlerin dağılımında Giardia intestinalis 6 (%4.4), Entamoeba histolytica 3 (%2.2), Taenia saginata 3 (%2.2), Ascaris lumbricoides 2 (% 1 .5) kişide, Hymenolepis nana ise 1 (%0.7) kişide saptanmıştır. İlimizdeki berberlerde yapılan bu çalışma berberlerde sık sık portör muayenelerinin yapılması gerektiği sonucunu ortaya koymuştur.The incidence of intestinal parasites differs from country to country and even from city to city with the eating habits of humans playing a role in this difference. Barbers may play a role in the spread of parasites. In order to determine the rate of parasitic infection in barbers, stool samples were collected from 137 barbers in Kırıkkale and examined macroscopically, and microscopically. In addition, anamneses of their complaints in relation to parasites were obtained. The native-Lugol method was used for microscopic examinations of stool samples. During this study carried out during April-June 2000, no parasites were found in the hair or on the hands of 137 male barbers whose ages ranged from 17 to 65 years. However, it was found that 15 of them had intestinal parasites. The types of parasites and their frequencies were as follows: 6 (4.4%) Giardia intestinalis, 3 (2.2 %) Entamoeba histolytica, 3 (2.2 %) Taenia saginata, 2 (1.5%) Ascaris lumbricoides, and 1 (0.7%) Hymenolepis nana. The findings of this study conducted in Kırıkkale indicated that examinations for the carriage of parasites by the barbers should be carried out frequentl

In vitro antimicrobial activity of commonly used vasoactive drugs

WOS: 000384952700087PubMed: 27687422Study Objective: Microbial contamination during preparation of the infusion drugs is an important issue in intensive care units. Objective of this study was to investigate in vitro antimicrobial properties of commonly used vasoactive drugs. Design: Prospective study. Setting: Clinical microbiology laboratory of a university hospital. Measurements: Growth of the microorganisms Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans in saline dilutions of adrenaline at 1, 10, and 100 mu g/mL; noradrenaline at 1, 10, and 100 mu g/mL; and dopamine at 0.1, 1, and 10 mg/mL concentrations was investigated. Each drug solution and saline were analyzed with a digital pH meter. Main Results: Saline dilutions of adrenaline, noradrenaline, and dopamine at clinically used concentrations decreased microbial growth. The highest concentration doses of adrenaline, noradrenaline, and dopamine used in the study had significant antimicrobial effect when compared to the low and moderate doses. This effect was shown with the all microorganisms. S aureus, S epidermidis, and C albicans were more sensitive; on the other hand, E coli and P aeruginosa were more resistant against the effect of the drug dilutions. Conclusions: To limit microbial growth in case of contamination of the drug solution, it is advisable to use more concentrated dilutions of adrenaline, noradrenaline, and dopamine used in clinical practice. (C) 2016 Elsevier Inc. All rights reserved

In vitro antimicrobial activity of commonly used vasoactive drugs

WOS: 000384952700087PubMed: 27687422Study Objective: Microbial contamination during preparation of the infusion drugs is an important issue in intensive care units. Objective of this study was to investigate in vitro antimicrobial properties of commonly used vasoactive drugs. Design: Prospective study. Setting: Clinical microbiology laboratory of a university hospital. Measurements: Growth of the microorganisms Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans in saline dilutions of adrenaline at 1, 10, and 100 mu g/mL; noradrenaline at 1, 10, and 100 mu g/mL; and dopamine at 0.1, 1, and 10 mg/mL concentrations was investigated. Each drug solution and saline were analyzed with a digital pH meter. Main Results: Saline dilutions of adrenaline, noradrenaline, and dopamine at clinically used concentrations decreased microbial growth. The highest concentration doses of adrenaline, noradrenaline, and dopamine used in the study had significant antimicrobial effect when compared to the low and moderate doses. This effect was shown with the all microorganisms. S aureus, S epidermidis, and C albicans were more sensitive; on the other hand, E coli and P aeruginosa were more resistant against the effect of the drug dilutions. Conclusions: To limit microbial growth in case of contamination of the drug solution, it is advisable to use more concentrated dilutions of adrenaline, noradrenaline, and dopamine used in clinical practice. (C) 2016 Elsevier Inc. All rights reserved

Echinococcosis: A possible etiology in psoriatic disease

WOS: 000242372000012PubMed: 17135741We aimed to investigate seroprevalence of Echinococcus granulosus in patients with psoriasis to determine a possible etiologic role, since both echinococcosis and psoriasis are defined as T cell-mediated diseases. Forty psoriatic patients and 50 age- and sex-matched control subjects were included in the study. IgG-specific ELISA was used to determine seropositivity. E. granulosus-specific IgG antibodies were found to be positive in 17/40 (42.5%) of the patients with psoriasis and in 11/50 (22%) of the control subjects (p = 0.008). Our results suggest that echinococcosis might be one of the causative pathogens in the etiopathogenesis of psoriasis in highly endemic regions. Copyright (c) 2006 S. Karger AG, Basel

Kırıkkale (Türkiye)' de insan, koyun ve sığır topluluklarında bruselloz seroprevalansı

Türkiye'de Kırıkkale'nin kırsal kesimlerinde insanlarda, koyun ve sığır topluluklarında Brusella serorpevalansı araştırıldı. Serum örnekleri 1436 kişiden elde edildi (% 28.41 erkek ve % 71,59 bayan). Toplumun % 3,2 (46) si Rose Bengal Plate testi (RBPT) ile ve % 3.0 (43)'ü standard tüp aglutinasyon testi (STAT) ile pozitif olarak sonuç verdi. RBPT ile pozitif olarak bulunan serumlar ELISA testi kullanılarak % 75 (33 kişi) Brucella ELISA IgG ile ve%25 (11 kişi) Brucella ELISA IgM ile pozitif olarak sonuç verdi. RBPT ile 301 sığırdan ve 503 koyundan olmak üzere toplam 804 serum örneğinin araştırılması sonucu % 47 (52) seropozitiflik gözlendi. RBPT Ne pozitif olarak tespit edilen tüm hayvanlar STAT ile de pozitif olarak sonuç verdi. 301 sığır ve 503 koyunda doğrulama testi olarak kullanılan Kompleman Fiksasyon Testi (CFT) ile seropozitiflik sırasıyla % 1 ve % 8 olarak bulundu.The seroprevalence of Brucellosis in human, sheep, and cattle populations was investigated in rural regions of Kırıkkale in Turkey. Serum samples were obtained from 1436 humans, and 3.2% (46) of the population was found to be positive by Rose Bengal Plate test (RBPT) and 3.0% {43) gave positive results with the standard tube agglutination test (STAT). Immunoglobulin class analysis of RBPT-positve sera using the ELISA test gave positive results for 33 people (75%) with Brucella ELISA IgG and for 11 people (25%) with Brucella ELISA IgM. The evaluation of 804 serum samples, which were obtained from 301 cattle and 503 sheep, showed 6.47% (52) seropositivity with RBPT. Additionally, all animals were found to be positive with STAT. The seropositivity was 1% and 8 %. respectively, in serum samples obtained from 301 cattle and 503 sheep with the complement fixation test (CFT), which was used as the confirmatory test

The possible association of Chlamydia pneumoniae infection with nasal polyps

WOS: 000243347400005PubMed: 16902779The objective of this study was to investigate the possible etiologic role of Chlamydia pneumoniae infection in nasal polyps. This prospective clinical study was undertaken enrolling 30 patients with nasal polyps, and 30 age and sex-matched control subjects from whom epithelial material was obtained during endoscopic surgery. IgG specific ELISA was used to determine seropositivity, and indirect immunoflorescence to determine C. pneumoniae in the biopsy material. C pneumoniae specific IgG antibodies were found to be positive in 16 (53.3%) of the patients with nasal polyps, and 9 (22%) of the control subjects (P = 0.065). Indirect immunofluorescence results indicate that there was a significant difference between groups; 16 (53.3%) patients in the study group and,8 (26.6%) patients in the control group were found to be positive (P = 0.034). We found no correlation with cigarette smoking. Our results indicated that Chlamydia pneumoniae is possibly associated with the presence of nasal polyps. However, these results remain to be confirmed with further studies using other techniques, and the underlying mechanism should be defined

Increased seropositivity of Helicobacter pylori cytotoxin-associated gene-A in Behcet's disease

WOS: 000246178800007PubMed: 17021670Behcet's disease is a systemic vasculitic syndrome with unknown etiology. The aim of the present study was to compare the Helicobacter pylori seropositivity and cytotoxin associated gene-A status in patients with Behcet's disease. Ninety-one patients with Behcet's disease and 83 age- and sex-matched persons with or without any gastrointestinal complaints were included in the study. Demographic characteristics and H. pylori IgG, IgM, and cytotoxin-associated gene-A IgG status of the Behcet's disease and the control groups were analyzed. The influence of eradication therapy on clinical findings was also determined. The prevalence of H. pylori IgG seropositivity was slightly but not significantly higher in patients with Behcet's disease compared to the controls [72 (79.1%) vs 56 (67.5%), (p=0.082)]. The prevalence of cytotoxin-associated gene A positivity was significantly higher in Behcet's disease compared to the controls [59 (64.8.%) vs 32 (38.5%), respectively, (p=0.002)]. Eradication of H. pylori has significantly decreased clinical manifestations such as oral and genital ulceration, arthritis/arthralgia, and cutaneous findings of Bahcet's disease. Our study indicates that H. pylori may be involved in the pathogenesis of Behcet's disease or disease activity might be enhanced due to induced inflammation or altered immunity

Seroprevalence of brucellosis in human, sheep, and cattle populations in Kirikkale (Turkey)

WOS: 000245467600013The seroprevalence of Brucellosis in human, sheep, and cattle populations was investigated in rural regions of Kirikkale in Turkey. Serum samples were obtained from 1436 humans, and 3.2% (46) of the population was found to be positive by Rose Bengal Plate test (RBPT) and 3.0% 43) gave positive results with the standard tube agglutination test (STAT). Immunoglobulin class analysis of RBPT- positive sera using the ELISA test gave positive results for 33 people (75%) with Brucella ELISA IgG and for 11 people (25%) with Brucella ELISA IgM. The evaluation of 804 serum samples, which were obtained from 301 cattle and 503 sheep, showed 6.47% (52) seropositivity with RBPT. Additionally, all animals were found to be positive with STAT. The seropositivity was 1% and 8 %, respectively, in serum samples obtained from 301 cattle and 503 sheep with the complement fixation test (CFT), which was used as the confirmatory test

The consistency of total immunoglobulin E with the symptoms according to age and the selection of allergy panels in Malatya (Turkey)

WOS: 000247519500004Distribution of allergens may change according to characteristics of regions. Measurements of total immunoglobulin E (TIE) and specific IgE antibodies are used to diagnose allergic diseases. In this study, we investigated sensitivity of TIE and its consistency with allergic symptoms, and compared the appropriateness of some allergy panels with features of Malatya, south-eastern Turkey province. Sera of 233 allergic patients of various age groups were tested for TIE. The specific IgE's were worked with 529 sera for food panel 5 (FP5) and one inhalant panel by using chemiluminesence technique. The sixty of inhalant panel positive sera were tested with specific IgE against house dust mites, Dermatophagoides pteronyssinus and Dermatophagoides farinae. The specific IgE against egg white, milk, wheat, corn, tomato, beef, strawberry and banana were investigated in sixty of FP5 positive sera by enzyme immunoassay method. The sensitivity of TIE was found to be 85%. We concluded that TIE can be used as a scanning test in children aged 5-18 years (69% positive) but it is not useful for 0-5 age group nor in adults (38%, 53% positive, respectively). The allergen scanning test panels should be designed according to custom of society and characteristics of the region