A Novel Method for Rearing Zebrafish by Using Freshwater Rotifers (Brachionus calyciflorus)

The zebrafish (Danio rerio) has become a powerful model organism for studying developmental processes and genetic diseases. However, there remain several problems in previous rearing methods. In this study, we demonstrate a novel method for rearing zebrafish larvae by using a new first food, freshwater rotifers (Brachionus calyciflorus). Feeding experiments indicated that freshwater rotifers are suitable as the first food for newly hatched larval fish. In addition, we revisited and improved a feeding schedule from 5 to 40 days postfertilization. Our feeding method using freshwater rotifers accelerated larval growth. At 49 dpf, one pair out of 10 pairs successfully produced six fertilized eggs. At 56, 63, and 71 dpf, 6 out of the 10 pairs constantly produced normal embryos. Our method will improve the husbandry of the zebrafish

Immune Modulation by Telomerase-Specific Oncolytic Adenovirus Synergistically Enhances Antitumor Efficacy with Anti-PD1 Antibody

The clinical benefit of monotherapy involving immune checkpoint inhibitors (ICIs) such as anti-programmed death-1 antibody (PD-1 Ab) is limited to small populations. We previously developed a telomerase-specific oncolytic adenovirus, Telomelysin (OBP-301), the safety of which was confirmed in a phase I clinical study. Here, we examined the potential of OBP-502, an OBP-301 variant, as an agent for inducing immunogenic cell death (ICD) and synergistically enhancing the efficacy of OBP-502 with PD-1 Ab using CT26 murine colon cancer and PAN02 murine pancreatic cancer cell lines. OBP-502 induced the release of ICD molecules such as adenosine triphosphate (ATP) and high-mobility group box protein 1 (HMGB1) from CT26 and PAN02 cells, leading to recruitment of CD8-positive lymphocytes and inhibition of Foxp3-positive lymphocyte infiltration into tumors. Combination therapy involving OBP-502 intratumoral administration and PD-1 Ab systemic administration significantly suppressed the growth of not only OBP-502-treated tumors but also tumors not treated with OBP-502 (so-called abscopal effect) in CT26 and PAN02 bilateral subcutaneous tumor models, in which active recruitment of CD8-positve lymphocytes was observed even in tumors not treated with OBP-502. This combined efficacy was similar to that observed in a CT26 rectal orthotopic tumor model involving liver metastases. In conclusion, telomerase-specific oncolytic adenoviruses are promising candidates for combined therapies with ICIs

The circadian clock is disrupted in mice with adenine-induced tubulointerstitial nephropathy.

Chronic Kidney Disease (CKD) is increasing in incidence and has become a worldwide health problem. Sleep disorders are prevalent in patients with CKD raising the possibility that these patients have a disorganized circadian timing system. Here, we examined the effect of adenine-induced tubulointerstitial nephropathy on the circadian system in mice. Compared to controls, adenine-treated mice showed serum biochemistry evidence of CKD as well as increased kidney expression of inflammation and fibrosis markers. Mice with CKD exhibited fragmented sleep behavior and locomotor activity, with lower degrees of cage activity compared to mice without CKD. On a molecular level, mice with CKD exhibited low amplitude rhythms in their central circadian clock as measured by bioluminescence in slices of the suprachiasmatic nucleus of PERIOD 2::LUCIFERASE mice. Whole animal imaging indicated that adenine treated mice also exhibited dampened oscillations in intact kidney, liver, and submandibular gland. Consistently, dampened circadian oscillations were observed in several circadian clock genes and clock-controlled genes in the kidney of the mice with CKD. Finally, mice with a genetically disrupted circadian clock (Clock mutants) were treated with adenine and compared to wild type control mice. The treatment evoked worse kidney damage as indicated by higher deposition of gelatinases (matrix metalloproteinase-2 and 9) and adenine metabolites in the kidney. Adenine also caused non-dipping hypertension and lower heart rate. Thus, our data indicate that central and peripheral circadian clocks are disrupted in the adenine-treated mice, and suggest that the disruption of the circadian clock accelerates CKD progression

パロディ ノ アル ニホンゴ キョウイク

パロディには、1)原典、2)模倣(敬愛)、3)作り変え(批評性)が不可欠である。また、多くの場合、4)滑稽味を伴う。その語源「パラ」には、対立と親密性の両義が含まれている。本稿はこうしたパロディの特徴、とりわけその二重性と批評性、カーニヴァル的な再生予祝機能に注目し、それを利用した日本語教授法を考察する。 四技能習得の手段としてパロディを用いる場合、とくに読み・書きでは、能動的・開放的な作業を促すことができる。それとともに、文学離れの現状や日本に対する固定観念に対処するためにも、パロディ使用は有効である。その場合、従来文学テキストを扱うときにありがちだった大作家や名作、日本らしさ志向、小説偏重に捕われずに、翻訳や読解以外の方法を積極的に取り入れたい。 例として、筒井康隆による『サラダ記念日』のヤクザ版パロディ、「カラダ記念日」と、井上ひさしの『吉里吉里人』の一節を使う授業を取り上げる。前者では、短歌や文語文法についての予備知識もない学生に、比較的短時間で本歌とパロディを比べさせ、自作のパロディ短歌を作らせるところまで持っていく。後者では、『坊っちゃん』他名作の冒頭の吉里吉里語訳と日本語の原文、すなわちパロディの原典とを比べ読むことによって、学習者は、日本人なら誰でも知っている(はずの)名文に触れるとともに、パロディ特有のカーニヴァル的転倒を体験することができる。 限られた授業時間では、長大な文を扱うことはできない。しかし、その場限りの断片ではなく、過去や未来とつながるものをめざしたい。それには、パロディが大いに役立つと考える。The aim of this article is to explore various possibilities of using parody for Japanese-language teaching. Parody requires: 1) a prior text, 2) imitation, and 3) transformation. While 2) is often associated with respect for 1), 3) is closely connected with the subversive and carnivalesque nature of parody. Parody pre-serves its object at the same time as it revolts against it. This structural and attitudinal ambivalence can also be explained etymologically: "para" means both "counter/against" and "beside/near." Given this double-sidedness, parody can be used as an effective tool in second-language teaching, reducing fear and encouraging active participation of the learner. Use of parody in language classes can also contribute to alleviating the general tendency away from literature and the stereotype preconceptions regarding Japan. Yet this is by no means an attempt to revive the traditional reading/translation of literary texts in language class. Texts should be selected not on the basis of their canonicity or "Japaneseness," but for their regenerative and critical merit. Genres other than fiction should also be taken into consideration. Two examples of using parodic texts for a late intermediate (or early advanced) Japanese course are presented. First, Tsutsui Yasutaka\u27s "Karada Kinenbi," which parodies Tawara Machi\u27s Salad Anniversary. In a relatively short period of time, students with no prior knowledge acquire a basic understanding of tanka, literary/poetic style, and parody to the extent that they can appreciate Tsutsui\u27s text and create their own parodies of Salad Anniversary. The second example involves a short excerpt from Inoue Hisashi\u27s Kirikirijin. Celebrated texts by Soseki, Kawabata, and others are "translated" into the Kirikiri language. These "translations" accompanied by the protagonist\u27s comments provide the students with an entertaining and dialogic introduction to the modern Japanese literary canon

Absolute Gravity Measurements and Gravity Changes at Syowa Station, Antarctica

第3回極域科学シンポジウム/第32回極域地学シンポジウム 11月29日（木） 統計数理研究所 ３階セミナー

Research Activities in the Department of Physical Therapy

[Introduction] It is already fifty years since the Japanese law of physical therapists and occupational therapists has been effective. The physical therapist is referred by the law as "the professionals who implements the physical therapy to persons with disabilities under the prescription of medical doctors". In fifty years, however, the target of physical therapy has been significantly expanded. The subject for physical therapy now includes the patients in acute disease just after the surgical operation in addition to those in rehabilitation stage. In other words, the physical therapy is now recognized as the indispensable intervention to the subject with acute as well as chronic disorders. On the other hand, due to a rapid transition of the society into the aged society, prevention of diseases, and decline of activity capacity due to the aging have become major issues for the physical therapy