Perinatal stem cells revisited: directions and indications at the crossroads between tissue regeneration and repair.

Perinatal stem cells research attracted great interest worldwide in recent years. Foetus-associated tissues contain various populations of stem cells, most of which are comprised within the category of mesenchymal stem cells (MSCs). This special issue collects both reviews and original reports on all the perinatal stem cell types which are currently under investigation. These cells have multiple promising features: differentiative capacity towards mature cell types of all the three germ layers, hypoimmunogenicity in vitro and in vivo, ease of sourcing, ex vivo culture and stor- age. In particular, immune modulation is viewed as a prom- ising feature of many MSCs populations, since these cells, once administered therapeutically, may be able to overcome, or at least evade, the host immune response which may lead to acute or chronic rejection of the transplant

A laser-scanning confocal microscopy study of carrageenan in red algae from seaweed farms near the Caribbean entrance of the Panama Canal

Kappaphycus alvarezii (Doty) Doty ex P.C. Silva, a red macroalga, is a commercial source of carrageenan, a widely used polysaccharide compound important in the food and pharmaceutical industries, in nanotechnology, and in pharmacological applications. Carrageenan is found mainly in the cell wall and in the intercellular matrix. This is the first study to propose the characterization of carrageenans in vitro, using the auto-fluorescence properties of the alga treated with different polyamines: putrescine, spermidine, and spermine. This study suggests a four-phase cultivation sequence for seaweed farmers to enhance and assess the potential carrageenan yield of their crops. In phase 1, seedlings were treated with each of the polyamines. Explants were subsequently transferred through two additional culture phases before being planted on the sea farms in phase 4 and then harvested after 60 days for analysis. Images from transverse sections of 11 representative cultured K. alvarezii samples were obtained at 561 nm excitation wavelength for both the cell center and the cell wall of each sample. Spectral data were also analyzed using the spectral phasor algorithm of SimFCS developed at the Laboratory for Fluorescence Dynamics (www.lfd.uci.edu). We report on the identification of several spectral fluorescence emission fingerprints from different auto-fluorescence compounds spatially mapped using this technique. These fingerprints have the potential to improve strain selection of explants for enhanced carrageenan yield in seaweed farming operations as well as to enable wholesale pricing to correspond with crop quality

School readiness of moderately preterm children at preschool age

The study investigates the preschool readiness of moderately preterm children and, in particular, the likely presence of learning disabilities at preschool age. Its theoretical model detects linguistic comprehension and expression; memory-related metacognition and cognition skills; orientation and motor coordination skills; premathematics and preliteracy ones. The research project involved an experimental group made up of 55 moderately preterm children (mean age = 62 months, mean gestational age = 34.6 weeks), without any clinical neonatal complications, and low birth weight (M = 2,100 g, SD = 350 g); a control group made up of 55 full-term children without pre- and perinatal complications. The children’s primary school reference teachers were given a questionnaire (Observative Questionnaire for Early Identification of Learning Disabilities), which was aimed at identifying any likely social and cognitive skill deficits at preschool age. The data, submitted to nonparametric univariate variance, show how scores of moderately preterm children in the metacognition [U (n1 e n2 = 55) = 35, p = 0.04, r = 0.49] are statistically lower, other cognitive skills (memory, orientation, and visual–motor coordination) [U (n1 e n2 = 55) = 32, p = 0.02, r = 0.45], premathematics [U (n1 e n2 = 55) = 32, p = 0.02, r = 0.44], and the total score [U (n1 e n2 = 55) = 31, p = 0.02, r = 0.12]. Such data would seem to suggest the need for hypothesizing preventive training and educational paths as a prerequisite for the development of the school abilities

Ten kilodalton heat shock protein (HSP10) is overexpressed during carcinogenesis of large bowel and uterine exocervix

In the present study, we evaluated the presence and the level of expression of HSP10 in two carcinogenetic models: the 'adenoma-carcinoma sequence' of large bowel and the 'dysplasia-carcinoma sequence' of uterine exocervix. We found HSP10 was overexpressed during the carcinogenesis of both organs. In particular, HSP10 was overexpressed early in large bowel carcinogenesis, while the expression of this protein in exocervical carcinogenesis gradually increased from normal through dysplastic to neoplastic tissues. The quantitative analysis of immunohistochemistry and the Western blotting confirmed these results. Our previous observations showed overexpression of HSP60 in the same carcinogenetic models. This report correlates the overexpression of HSP10 with that of HSP60 during carcinogenesis in vivo. These results could stimulate further studies on the pathogenetic role of these proteins during the carcinogenesis as well as their use as diagnostic and prognostic tools in oncology. \ua9 2003 Elsevier Science Ltd. All rights reserved

Dermoscopy and methyl aminolevulinate: A study for detection and evaluation of field cancerization

Actinic keratosis (AK) is a keratinocyte intraepidermal neoplasia UV light – induced that frequently appears in sun-exposed areas of the skin. Although historically AK was de fi ned as “ precancerous ” , actually it is considered as the earliest stage of squamous cell carcinoma (SCC) in situ. Since AKs can progress into invasive SCC, their treatment isrecommended. AKsrarely developasa singlelesion;usually multiplelesions commonly affect anen- tire area of chronically actinic damaged skin. This has led to the concept of “ fi eld cancerization ” , an area chroni- cally sun-exposed that surrounds peripherally visible lesions, in which are individualized subclinical alterations. One of the main principles endpoint in the management of AKs is the evaluation and the treatment of fi eld cancerization. In this view, in order to detect and quantify fi eld cancerization, we employed a method based on the topical application of methyl aminolevulinate (MAL) and the detection of the fl uorescence emitted by its metabolite Protoporphyrin IX (PpIX); then, considering the extension and the intensity of measured fl uores- cence, we create a score of fi eld cancerization. The results show that patients underwent to daylight PDT had a reduction of total score, from T0 to T2. Whereas in the group untreated we observed a stability of total score or a slightly worse. So, the method and the score used allows to evaluate with a good approximation the dimension of fi eld cancerization and show the modi fi cation of it after treatment

Free and open-source control software for 3-D motion and processing

RepRap 3-D printers and their derivatives using conventional firmware are limited by: 1) requiring technical knowledge, 2) poor resilience with unreliable hardware, and 3) poor integration in complicated systems. In this paper, a new control system called Franklin, for CNC machines in general and 3-D printers specifically, is presented that enables web-based three dimensional control of additive, subtractive and analytical tools from any Internet connected device. Franklin can be set up and controlled entirely from a web interface; it uses a custom protocol which allows it to continue printing when the connection is temporarily lost, and allows communication with scripts

Multiparametric advanced research tool for meteo satellites data interfacing with space observation of ultra high energy cosmic rays

To approach the study of the cosmic rays in the energy range E > 1020 eV, the upper end of the spectrum observed to date, with a large statistical significance (103 events/year), and hence address the solution of several astrophysical and cosmological problems related to their existence and behaviour, a new generation of experiments will probably have to be conceived and realised. They will be based on the observation and measurements of cosmic rays from space. The extremely low rate of these events (∼ 1 event/(century × km2 × sr)) imposes a very large effective area to be monitored, of the order of 105 km2, as an observational requirement to meet the target statistics. The Extreme Universe Space Observatory (EUSO)mission has been proposed as the precursor of this new generation of experiments. Its approach consists in fact in looking downwards to the Earth atmosphere by means of a large field-of-view telescope accommodated aboard an orbiting satellite. The fluorescence strike produced by a cosmic ray through the atmosphere will be recorded by the detector, which will reconstruct the kinematical and dynamical features of the primary cosmic ray. The atmosphere acts therefore as an active target for the detectable event. A strategic tool for the success of EUSO as well as for all the experiments of its category will be a correct and detailed atmospheric sounding system, in order to monitor the atmospheric parameters within the field-of-view of the telescope. Beside an on-board measurement by means of dedicated devices such an infrared camera (IR)and possibly a LIDAR (LIght Detection And Ranging)coupled to the main instrument, the Atmosphere Sounding will take advantage from the continuous observation of the atmospheric parameters given by the orbiting meteorological satellites. Their databases have thus to be interfaced to the experimental data and used picking-up the relevant data according to the space and time coordinates corresponding to each triggered event. The present work outlines a software module (MARVIN-Multiparametric Advanced Research tool for Visualisation In the Network) able to build-up such an interface, and shows a preliminary implementation of it, using a sample of existing satellites and ISCCP meteorological data collection. It has been developed during the phase A study of the EUSO mission but is general enough to be adapted to different missions observing the Earth atmosphere from space

Publisher's Note: Defect Influence on Heteroepitaxial 3C-SiC Young's Modulus [Electrochem. Solid-State Lett., 14, H161 (2011)]

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Thoracoscopy in pleural effusion –two techniques: awake single-access video-assisted thoracic surgery versus 2-ports video-assisted thoracic surgery under general anesthesia

Awake single access video-assisted thoracic surgery with local anesthesia improves procedure tolerance, reduces postoperative stay and costs. MATERIALS & METHODS: Local anesthesia was made with lidocaine and ropivacaine. We realize one 20 mm incision for the 'single-access', and two incisions for the '2-trocars technique'. RESULTS: Mortality rate was 0% in both groups. Postoperative stay: 3dd ± 4 versus 4dd ± 5, mean operative time: 39 min versus 37 min (p < 0.05). Chest tube duration: 2dd ± 5 versus 3dd ± 6. COMPLICATIONS: 11/95 versus 10/79. CONCLUSION: Awake technique reduce postoperative hospital stay and chest drainage duration, similar complications and recurrence rate. The authors can say that 'awake single-access VATS' is an optimal diagnostic and therapeutic tool for the management of pleural effusions, but above extends surgical indication to high-risk patients

Probiogenomics analysis of 97 lactobacillus crispatus strains as a tool for the identification of promising next-generation probiotics

Members of the genus Lactobacillus represent the most common colonizers of the human vagina and are well-known for preserving vaginal health and contrasting the colonization of oppor-tunistic pathogens. Remarkably, high abundance of Lactobacillus crispatus in the vaginal environment has been linked to vaginal health, leading to the widespread use of many L. crispatus strains as probi-otics. Nevertheless, despite the scientific and industrial relevance of this species, a comprehensive investigation of the genomics of L. crispatus taxon is still missing. For this reason, we have performed a comparative genomics analysis of 97 L. crispatus strains, encompassing 16 strains sequenced in the framework of this study alongside 81 additional publicly available genome sequences. Thus, allowing the dissection of the L. crispatus pan-genome and core-genome followed by a comprehensive phylogenetic analysis based on the predicted core genes that revealed clustering based on ecological origin. Subsequently, a genomics-targeted approach, i.e., probiogenomics analysis, was applied for in-depth analysis of the eight L. crispatus strains of human origin sequenced in this study. In detail their genetic repertoire was screened for strain-specific genes responsible for phenotypic features that may guide the identification of optimal candidates for next-generation probiotics. The latter includes bacteriocin production, carbohydrates transport and metabolism, as well as a range of features that may be responsible for improved ecological fitness. In silico results regarding the genetic repertoire involved in carbohydrate metabolism were also validated by growth assays on a range of sugars, leading to the selection of putative novel probiotic strains