Conocimientos, Actitudes y Prácticas sobre lactancia materna que poseen las madres que asisten al programa de Vigilancia promoción, crecimiento y desarrollo en el centro salud de Villa Libertad, Managua. Octubre -Diciembre 2015

Se determinaron los conocimientos, actitudes y prácticas sobre lactancia materna que poseen las madres que asisten al programa de Vigilancia, Promoción, Crecimiento y Desarrollo, mediante un estudio descriptivo de corte transversal, probabilístico, realizado en 73 mujeres en rangos de edad de 15 a 42 años, seleccionadas por muestreo aleatorio simple en el Centro de Salud Villa Libertad de la ciudad de Managua en el periodo de Octubre a Diciembre del año 2015. Una vez seleccionada el área de estudio se realizó coordinación con personal de salud y docente a cargo, se identificó el problema objeto de la investigación, se procedió a la elaboración de los objetivos y del instrumento de recolección de datos, posteriormente se hizo la validación del mismo. La entrada al escenario de trabajo para la evaluación se realizó aplicando el formulario conteniendo las variables de acuerdo a cada objetivo específico, los cuales están enmarcados en cuanto a identificar información sociodemográfica, de conocimientos, actitudes y prácticas sobre lactancia materna. Se utilizaron medios y programas informáticos para el proceso de recolección y análisis de datos. Una vez analizada la información se obtuvieron los resultados, donde encontramos que las mujeres estudiadas manifestaron tener adecuados conocimientos sobre lactancia materna obtenidos en la unidad de salud donde han sido atendidas, tienen una actitud favorable, pero la práctica de lactancia materna es inadecuada, porque es mixta debido a que además de la leche materna se implementa el uso de fórmulas lácteas y alimentación complementaria a temprana edad. Palabras Clave: Lactancia Materna, Conocimientos, Actitudes, Prácticas, Fórmul

Golden Ratio Speech Codec (img)

<p>Original speech signal and Golden ratio speech codec resultant speech signal.</p

Assessing Organizational Users’ Intentions and Behavior to AI Integrated CRM Systems: a Meta-UTAUT Approach

This paper tests the meta-analysis based unified theory of acceptance and use of technology (meta-UTAUT) model to predict the behavioral intentions of organizational users and their use behavior to artificial intelligence (AI) integrated customer relationship management (CRM) systems. Data was collected from 315 organizational users in India. The hypotheses draw on the theoretical underpinnings which have been statistically validated. Results show that CRM quality and satisfaction significantly influences an organization’s employees attitudes and intentions to use AI integrated CRM systems. The compatibility of CRM systems has, however, a limited impact on employees attitudes. The findings, which are aligned with the extended UTAUT model, provide useful insights into organizations and decision-makers for designing AI integrated CRM systems.Other Information Published in: Information Systems Frontiers License: https://creativecommons.org/licenses/by/4.0See article on publisher's website: http://dx.doi.org/10.1007/s10796-021-10181-1</p

Differential expression of microRNAs in the brains of mice subjected to increasing grade of mild traumatic brain injury

<p><i>Objective</i>: To investigate the effect of heterogeneity in mTBI on miRNA expression in mouse brain and to identify molecular pathways targeted by the modulated miRNAs.</p> <p><i>Methods</i>: A weight drop device was used to induce four increasing grades of mTBI. MiRNA expression was evaluated using TaqMan rodent miRNA arrays. Bioinformatics analysis was done using the DIANA miRPath tool and Ingenuity Pathway Analysis software. Histology of brain sections was evaluated using H&E staining.</p> <p><i>Results</i>: No histologic lesions were observed in the brains of injured mice; however, significant modulation in miRNA expression profile was observed. Global miRNA profiling indicated a trend of decrease in the number of modulated miRNAs from 24 hours to day 7 post-injury, except for the most severe grade of mTBI. Canonical pathways like calcium signalling, synaptic pathways and axon guidance pathway were the major targets of the modulated miRNAs. Network correlation analyses indicated an interaction between the modulated miRNAs and putative protein biomarkers of TBI.</p> <p><i>Conclusions</i>: The data demonstrated that varying intensities of mTBI induced a differential miRNA expression profile in the brain post-injury. Pathways such as calcium and synaptic signalling were major targets of modulated miRNAs and may play a role in the pathophysiology of mTBI.</p

Method and system for identifying a person using their finger-joint print

Inventor name used in this publication: 张磊Inventor name used in this publication: 张林Inventor name used in this publication: 祝海龙Inventor name used in this publication: 张大鹏Inventor name used in this publication: 骆南Title in Traditional Chinese: 利用指關節紋識別個人身份的方法和系統China2012-2013 > Other Outputs > Patents grantedVersion of Recor

Additional file 1: Figure S1. of Differential host gene responses from infection with neurovirulent and partially-neurovirulent strains of Venezuelan equine encephalitis virus

Venn diagram with comparison of gene expression at different time points in a) V3000 infected spleen, b) V3034 infected spleen, c) V3000 infected brain, and d) V3034 infected brain. Significantly modulated genes against each virus at different time points studied were compared in spleen and brain. The genes representing unique or common subsets are shown in the venn diagrams. The numbers in the venn diagrams include both upregulated and downregulated genes. Figure S2. Real-time PCR based validation. Real-time PCR analysis was performed to confirm the microarray results for randomly selected genes a) Stat1, b) Stat2, c) Zfp456, Nt5c2 and NfκB2 post V3000 infection and d) Samd9l post V3000 and V3034 infections. Expression values of all the genes were normalized with the house keeping gene, GAPDH. The results here are representative of 2 biological replicates and 2 technical replicates for each biological replicate. Blue bar: RT-PCR expression level; Red bar: Microarray expression level. Details of primer sets used are given in supplementary table-7. Figure S3. Network analysis of apoptotic genes modulated in response to V3000 and V3034 infections in spleen at 48 h and 72 h pi. Genes significantly modulated against V3000 and V3034 infections at 48 h and 72 h pi in spleen were used to perform in silico network analysis using the Ingenuity Pathway analysis software. Both the viruses resulted in modulation of apoptosis pathway at 48 h and 72 h pi. Figure S4. Network analysis of inflammatory genes modulated in response to V3000 and V3034 infections in spleen at 48 h and 72 h pi. Genes significantly modulated against V3000 and V3034 infections at 48 h and 72 h pi in spleen were used to perform in silico network analysis using the Ingenuity Pathway analysis software. Both the viruses resulted in activation of various immune cells to different degrees at 48 h and 72 h pi as shown above. (DOCX 1842 kb

Additional file 4: Table S3. of Differential host gene responses from infection with neurovirulent and partially-neurovirulent strains of Venezuelan equine encephalitis virus

Significantly modulated genes in the spleen that were unique to V3000 infection. Genes that were modulated only with V3000 infection in the spleen were identified. The list summarizes the commonly modulated genes for each time point studied. Values are expressed as average values of (log2) fold expression for each gene over uninfected controls ± standard error mean (SEM). * P ≤ 0.05. (DOCX 82 kb

Additional file 5: Table S4. of Differential host gene responses from infection with neurovirulent and partially-neurovirulent strains of Venezuelan equine encephalitis virus

Significantly modulated genes in the brain that were unique to V3000 infection. Genes that were modulated only with V3000 infection in the brain were identified. The list summarizes the commonly modulated genes for each time point studied. Values are expressed as average values of (log2) fold expression for each gene over uninfected controls ± standard error mean (SEM). * P ≤ 0.05. (DOCX 38 kb

Identification of Serum MicroRNA Signatures for Diagnosis of Mild Traumatic Brain Injury in a Closed Head Injury Model

<div><p>Wars in Iraq and Afghanistan have highlighted the problems of diagnosis and treatment of mild traumatic brain injury (mTBI). MTBI is a heterogeneous injury that may lead to the development of neurological and behavioral disorders. In the absence of specific diagnostic markers, mTBI is often unnoticed or misdiagnosed. In this study, mice were induced with increasing levels of mTBI and microRNA (miRNA) changes in the serum were determined. MTBI was induced by varying weight and fall height of the impactor rod resulting in four different severity grades of the mTBI. Injuries were characterized as mild by assessing with the neurobehavioral severity scale-revised (NSS-R) at day 1 post injury. Open field locomotion and acoustic startle response showed behavioral and sensory motor deficits in 3 of the 4 injury groups at day 1 post injury. All of the animals recovered after day 1 with no significant neurobehavioral alteration by day 30 post injury. Serum microRNA (miRNA) profiles clearly differentiated injured from uninjured animals. Overall, the number of miRNAs that were significantly modulated in injured animals over the sham controls increased with the severity of the injury. Thirteen miRNAs were found to identify mTBI regardless of its severity within the mild spectrum of injury. Bioinformatics analyses revealed that the more severe brain injuries were associated with a greater number of miRNAs involved in brain related functions. The evaluation of serum miRNA may help to identify the severity of brain injury and the risk of developing adverse effects after TBI.</p></div

Expression of miRNAs in individual real time PCR assay.

<p>The fold upregulation of three miRNAs, miR-376a, miR-214 and miR-199a-3p, in the injury groups over the sham mice was validated using the individual real time PCR assays. Similar to the miRNA arrays, expression of the three miRNAs was found to be up regulated in the injured groups over the sham animals. Data presented is the fold up regulation (± SEM; *<i>P</i><0.05) calculated from the mean DDCt value obtained from three biological samples for each injury group. Statistical significance was calculated using the individual Ct values obtained from the three biological replicates for each injury group.</p