4 research outputs found
Additional file 1: of Convergent synthesis of 13N-labelled Peptidic structures using aqueous [13N]NH3
Electronic Supplementary Information. (DOCX 77Â kb
One-Pot Multi-Tracer Synthesis of Novel <sup>18</sup>F‑Labeled PET Imaging Agents
<sup>18</sup>F labeled phosphonium salts are increasingly important
molecular probes for targeting the mitochondrial membrane potential
depletion during apoptosis and for detecting myocardial perfusion
deficit. Here, we introduce three new tracers, [<sup>18</sup>F]MitoPhos_04,
[<sup>18</sup>F]MitoPhos_05, and [<sup>18</sup>F]MitoPhos_07, that
have the potential to act as mitochondrial imaging agents. Moreover,
they have the added advantage of being synthesized in the same reaction
vial from one radiolabeled synthon, demonstrating a new approach to
synthesizing multiple tracers in one-pot, which is a highly useful
means for increasing the throughput of radiotracer development. The
radiosynthesis of the tracers was carried out on a fully automated
system via a facile two-step reaction. Utilizing the radiolabeling
of an ethyl azide, a copper-mediated 1,3-cycloaddition reaction and
isolation via semiprep high-performance liquid chromatography (HPLC)
allowed for the simultaneous synthesis of two or three tracers with
a total synthesis time of less than 1 h
Lipid hydrolysis by raclopride tartrate
<p>HPLC data showing the rates of lipid ester hydrolysis by a zwitterionic D2 antagonic, raclopride tartrate. Data is expressed as the molar percentage of lyso-lipid in the sample at the time of quenching (by freeze-drying of aliquot): fatty acid signal was not easily quantifiable. However, linear increase of lyso-lipid signal meant fatty acid concentration could be assumed to be equal.</p>
<p>System was deliberately chosen to deliver slow rates to make observation, sampling and quantitative analysis possible. This was generated as a follow-up to the original discovery, available at http://www.ncbi.nlm.nih.gov/pubmed/16973478.</p
The Spectroscopic Study of Cold Ions in Plasma and Ion Trap
%ID/g for the thyroid (upper), salivary glands (centre) and stomach (lower) in BALB/c mice estimated by ex vivo biodistribution (open circles) and PET ROI analysis (filled squares) at varying doses of 18/19F-BF4 â. (PDF 15.7 KB)