Numerical Simulation of Droplet Impact on Wettability-Patterned Surfaces

The analysis of droplet impact on micro-structured surfaces is a very challenging problem since it involves several physical phenomena interacting with one each other at levels of correlation depending on space and time. Moreover, understanding the dynamics and the main parameters a ecting the droplet behavior can give the capability to control and take advantage of its potential in di erent practical applications. In this work, rstly a phenomenological investigation of the phenomenon is provided involving models present in the literature and proposed theoretical analyses. Some fundamentals regarding numerical methods used in Computational Fluids Dynamics are presented in order to give the basis for the simulation set-up: particular attention is given to the contact angle boundary condition. Successively, several case studies are run with the open source software OpenFOAM: impacts on both surfaces with uniform and heterogeneous wettability characteristics are numerically simulated, with particular attention to cases exhibiting "morphing" and "vectoring" outcomes

Table1_Risk of cardiovascular toxicity with combination of immune-checkpoint inhibitors and angiogenesis inhibitors: a meta-analysis.docx

IntroductionCombinations of immune checkpoint inhibitors (ICIs) and angiogenesis inhibitors (AIs) have been investigated for the treatment of several tumor types. Both ICIs and AIs may lead to cardiovascular adverse events, and their combination may potentially increase the risk for cardiovascular toxicity. In the present meta-analysis, we aim to assess the cardiovascular toxicity of ICIs plus AIs vs. AIs alone. Secondary objectives are non-cardiovascular adverse events and efficacy.MethodsSystematic review was performed according to PRISMA statement. Phase II and III randomized clinical trials were identified by searching the MEDLINE/PubMed, Cochrane Library and ASCO Meeting abstracts, from inception to June 2022. The pooled risks for overall response rate (ORR), 1-year progression-free survival (PFS), adverse events (AEs), immune-related AEs, (irAEs), hypertension, and vascular events defined as stroke, myocardial infarction and pulmonary embolisms, were calculated.ResultsIn terms of cardiovascular toxicity, we found higher risk for severe hypertension among patients treated with ICIs plus AIs as compared with those receiving AIs (OR 1.24, 95% CI: 1.01–1.53), but no significant difference was found for any-grade hypertension, and for vascular events. There was also no difference in terms of overall AEs, whereas the incidence of irAEs was increased in the ICIs plus AIs arm, as expected. In terms of efficacy, ICIs plus AIs achieved better ORR (OR 2.25, 95% CI: 1.70–2.97) and PFS (HR 0.49, 95% CI: 0.39–0.63) as compared to AIs alone.ConclusionThe addition of ICIs to AIs significantly increased the risk of high-grade hypertension, but not that of acute vascular events.</p

Supplemental material for Rare primary headaches in Italian tertiary Headache Centres: Three year nationwide retrospective data from the RegistRare Network

<p>Supplemental material for Rare primary headaches in Italian tertiary Headache Centres: Three year nationwide retrospective data from the RegistRare Network by Chiara Lupi, Luana Evangelista, Valentina Favoni, Antonio Granato, Andrea Negro, Lanfranco Pellesi, Raffaele Ornello, Antonio Russo, Sabina Cevoli, Simona Guerzoni and Silvia Benemei in Cephalalgia</p

Additional file 1: of The combined effect of subcutaneous granulocyte- colony stimulating factor and myocardial contrast echocardiography with intravenous infusion of sulfur hexafluoride on post-infarction left ventricular function, the RIGENERA 2.0 trial: study protocol for a randomized controlled trial

Procedure schedule and description of study assessments. Description of data: in this file a detailed description of the study assessments and their schedule is provided. (PDF 224 kb

Supplementary material 1 -Supplemental material for Prospective memory is dysfunctional in migraine without aura

<p>Supplemental material, Supplementary material 1 for Prospective memory is dysfunctional in migraine without aura by Gabriella Santangelo, Antonio Russo, Alessandro Tessitore, Federica Garramone, Marcello Silvestro, Maria Rosaria Della Mura, Laura Marcuccio, Ilaria Fornaro, Luigi Trojano, Gioacchino Tedeschi in Cephalalgia</p

sj-docx-7-tam-10.1177_17588359221110162 – Supplemental material for The diagnostic accuracy of PIK3CA mutations by circulating tumor DNA in breast cancer: an individual patient data meta-analysis

Supplemental material, sj-docx-7-tam-10.1177_17588359221110162 for The diagnostic accuracy of PIK3CA mutations by circulating tumor DNA in breast cancer: an individual patient data meta-analysis by Antonio Galvano, Luisa Castellana, Valerio Gristina, Maria La Mantia, Lavinia Insalaco, Nadia Barraco, Alessandro Perez, Sofia Cutaia, Valentina Calò, Tancredi Didier Bazan Russo, Edoardo Francini, Lorena Incorvaia, Mario Giuseppe Mirisola, Salvatore Vieni, Christian Rolfo, Viviana Bazan and Antonio Russo in Therapeutic Advances in Medical Oncology</p

sj-docx-6-tam-10.1177_17588359221110162 – Supplemental material for The diagnostic accuracy of PIK3CA mutations by circulating tumor DNA in breast cancer: an individual patient data meta-analysis

Supplemental material, sj-docx-6-tam-10.1177_17588359221110162 for The diagnostic accuracy of PIK3CA mutations by circulating tumor DNA in breast cancer: an individual patient data meta-analysis by Antonio Galvano, Luisa Castellana, Valerio Gristina, Maria La Mantia, Lavinia Insalaco, Nadia Barraco, Alessandro Perez, Sofia Cutaia, Valentina Calò, Tancredi Didier Bazan Russo, Edoardo Francini, Lorena Incorvaia, Mario Giuseppe Mirisola, Salvatore Vieni, Christian Rolfo, Viviana Bazan and Antonio Russo in Therapeutic Advances in Medical Oncology</p

sj-docx-9-tam-10.1177_17588359221110162 – Supplemental material for The diagnostic accuracy of PIK3CA mutations by circulating tumor DNA in breast cancer: an individual patient data meta-analysis

Supplemental material, sj-docx-9-tam-10.1177_17588359221110162 for The diagnostic accuracy of PIK3CA mutations by circulating tumor DNA in breast cancer: an individual patient data meta-analysis by Antonio Galvano, Luisa Castellana, Valerio Gristina, Maria La Mantia, Lavinia Insalaco, Nadia Barraco, Alessandro Perez, Sofia Cutaia, Valentina Calò, Tancredi Didier Bazan Russo, Edoardo Francini, Lorena Incorvaia, Mario Giuseppe Mirisola, Salvatore Vieni, Christian Rolfo, Viviana Bazan and Antonio Russo in Therapeutic Advances in Medical Oncology</p

mRNA and protein levels of IRS1, <i>c-MYC</i>, insRß, IGF1R and ß-catenin in paired colonic mucosa and primary colorectal cancer (CRC).

<p>Panel A shows histograms of the relative expression of <i>IRS1</i> (left) and <i>c-MYC</i> (right) transcripts in paired samples of cancer-unaffected colorectal mucosa (white) and CRC (black), as determined by quantitative real-time PCR (RTqPCR). Mucosa samples were set equal to 100% and normalized to the relative expression of the housekeeping gene, <i>Cyclophilin</i>. Cancer samples were expressed relative to mucosa and normalized to the relative expression of the housekeeping gene. In pairs M1T1 to M4T4 and in M8T8, both <i>c-MYC</i> and <i>IRS1</i> increase in CRC relative to mucosa, only in M5T5 and M6T6 <i>IRS1</i> and <i>c-MYC</i> disagree (<i>IRS1</i>: <i>P</i> = 0.05, <i>c-MYC</i>: <i>P</i><0.001, unpaired t test on the means of all differences, data not shown). Panel B shows western blot analysis of IRS1, beta subunit of the insulin receptor (InsRß), beta subunit of the insulin-like growth factor 1 receptor (IGF1Rß), ß-catenin and ß-actin, as loading control, in the paired colonic mucosa and CRC samples shown in A (except M6T6, for which tissue for western blot analysis was not available). The histograms in Panel C show quantitations, after normalization for ß-actin, of the IRS1, InsRß, IGF1Rß and ß-catenin signals. Relative to paired mucosa, IRS1 is overexpressed in the CRCs of pairs M1T1-M4T4, together with InsRß, IGF1Rß and ß-catenin (IRS1: <i>P</i> = 0.017, InsRß: <i>P</i> = 0.044, IGF1Rß: <i>P</i><0.001, ß-catenin: <i>P</i><0.001, unpaired t test on the means of all differences, data not shown). Notably, the CRCs that overexpressed the IRS1, InsRß, IGF1Rß and ß-catenin proteins also overexpressed <i>IRS1</i> and c-<i>MYC</i> mRNA.</p

Expression of IRS1, insulin receptor, IGF1 receptor and ultrastructural differentiation in polarizing Caco-2 cells.

<p>Panel A shows western blot analysis of IRS1, beta subunit of the insulin receptor (InsRß), beta subunit of the insulin-like growth factor 1 receptor (IGF1Rß) and ß-actin, as loading control, in Caco-2 cells at days 3, 7 and 14 post-confluence, duplicated in absence (−) and presence (+) of serum in the culture medium. The histograms show quantitations, after normalization for ß-actin, of the IRS1, InsRß and IGF1Rß signals (means ± SE from the two experiments). Under both culture conditions increased espression of IRS1 and InsRß is clearly evident in polarized cells at day 14 (IRS1) and at days 7 and 14 (InsRß), whereas maximum expression of IGF1Rß is detected only at day 3. Transmission electron microscopy of Caco-2 cells at day 3 of the spontaneous polarization time course reveals forming electron-dense junctions at the apex of the lateral membranes of adjacent cells (panel A, arrow). With progression of polarization, tight junctions and desmosomes (panels C–D, arrows) and adhesion junctions (panel D) become evident as electron-dense plaques on adjacent lateral membranes at days 7 and 14, respectively. In addition, tight multicellular clusters, with differentiation features, such as intracellular lumina rich of apical brush border (panels E–F), become evident at day 14. Abbreviations: tj, tight junction; ad, adhesion junction; ds, desmosome. Panel G shows western blot levels of tyrosine 632-phosphorylated IRS1 (IRS1tyr632) and, as loading control, ß-actin, in serum-starved Caco-2 cells unstimulated (−) and stimulated (+) with insulin (100 nM) or IGF1 (10 nM). IRS1 tyrosine phosphorylation is relevant at day 7 of polarization, independently from the addition of exogenous insulin or IGF1. However, at day 3, only exogenous IGF1 determines IRS1 phosphorylation.</p