Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga

The aim of this study was to evaluate the histology and morphometry of endochondral ossification in limbs of rats submitted to usnic acid from Cladonia substellata (AHTI) encapsulated in PLGA-microspheres. It was used 12 pregnant Wistar rats that were randomly distributed in the control groups (n = 6), which received 1.0 mL of saline solution; and treated (n = 6), that received 25 mg/kg/day of encapsulated usnic acid in PLGA microspheres by oral administration. It was analyzed six fetuses from each rat under study. At the 20th day of gestation were euthanized the females and their fetuses removed for histomorphometric analysis of the anterior and posterior limbs. The results showed the epiphyseal discs of the animals with cell alterations, as in the morphology and numbers of chondrocytes. It also showed a decrease in the amount of collagen type I collagen in bone tissue. However, this study showed low toxicity of the encapsulated usnic acid on bone development, when compared to its conventional dosage form. In this way, the toxicity of usnic acid can be reduced by encapsulation in PLGA-microspheres

Effects of light-to-moderate alcohol consumption on steatosis and steatohepatitis in severely obese patients.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-06-11T16:42:30Z No. of bitstreams: 1 Cotrim HP Effects of light-to....pdf: 87293 bytes, checksum: f939fbfab25416e2ba3f949679789879 (MD5)Made available in DSpace on 2014-06-11T16:42:30Z (GMT). No. of bitstreams: 1 Cotrim HP Effects of light-to....pdf: 87293 bytes, checksum: f939fbfab25416e2ba3f949679789879 (MD5) Previous issue date: 2009Universidade Federal da Bahia. Postgraduate Course in Medicine and Health (PPGMS). Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilCenter for the Surgical Treatment of Obesity. Salvador, BA, BrasilUniversidade Federal da Bahia. Postgraduate Course in Medicine and Health (PPGMS). Salvador, BA, BrasilUniversidade Federal da Bahia. Postgraduate Course in Medicine and Health (PPGMS). Salvador, BA, BrasilDivision of GI/Hepatology. University of Virginia. Charlottesville, USAOBJECTIVES: The effect of light-to-moderate alcohol consumption (LMAC) in nonalcoholic fatty liver disease (NAFLD) remains a controversial subject. The aim of this study was to evaluate the relationship between LMAC and the severity of NAFLD in morbidly obese patients. METHODS: We studied 132 patients undergoing liver biopsy during bariatric surgery. The patients were divided into three groups: G1: alcohol intake greater than 20 g/day and less than 40 g/day; G2: alcohol intake less than 20 g/day; G3: no alcohol intake. Insulin resistance was defined by the Homeostasis Model Assessment (>3). NAFLD was classified according to the Matteoni types: type I: steatosis alone; type II: steatosis with inflammation; types III-IV: steatosis with ballooning and/or fibrosis. RESULTS: The mean age was 37.3+/-11 years. Sixty-three percent were females and body mass index was 43.9+/-5.6 kg/m. G1, G2, and G3 included 19, 56, and 57 patients, respectively. Histological diagnoses classified by levels of alcohol were: G1: 10.5% normal liver, 89.5% type III or IV; G2: 10.7% normal liver, 1.8% type I or II, and 87.5% grade III or IV; G3: 10.5% normal liver, 3.5% type I or II, and 86% type III or IV (one had cirrhosis). The presence of IR was similar in moderate and no alcohol consumption (81.3 and 78.7%) but significantly less in the light consumption group (54%, P<0.05). CONCLUSION: The results suggest that LMAC may have a protection effect against IR in severely obese patients. However, it had no impact on the severity of activity and stage of liver disease

Nonalcoholic fatty liver disease in severely obese individuals: The influence of bariatric surgery

Background: Obesity is the most frequent risk factor associated with NAFLD, and bariatric surgery (BAS) is traditionally indicated for the treatment of severely obese individuals. Here, we discuss the behavior and prognosis of this liver disease following post-surgical weight loss. Aim: To evaluate the influence of the BAS on the clinical and biochemical parameters of NAFLD in severely obese patients. Methodology: An intervention study included obese individuals (BMI≥ 35kg/m2), who had been submitted to liver biopsy during BAS and had NAFLD. HAIR (hypertension, ALT and insulin resistance and BAAT (BMI, ALT, age and triglycerides) scores and FLI (Fatty Liver Index) were used to compare the patients at the time of surgery, and 12-30 months following weight loss. Results: From October 2004 to September 2007, 122 patients were diagnosed with NAFLD, 40 of whom agreed to participate in the study. The mean age was 37.7 ± 12.5 years, 60% were women and 80% had steatohepatitis (NASH) with fibrosis upon analysis of the liver biopsy performed during BAS. Mean weight loss was 46.0 ± 2.0 kg. After 21 ± 5.8 months of follow-up, a significant improvement was found in all the variables analyzed (79.3% according to the HAIR scores, 95.2% as measured by the BAAT score and 72.5% by the FLI. Conclusion: The results suggest that treatment of obesity by bariatric surgery may influence the prognosis of NAFLD. In addition to weight loss, we observed improvement in the clinical and biochemical parameters related to NAFLD, such as anthropometrics index, hypertension, aminotransferases, triglycerides and insulin resistance

Nonalcoholic fatty liver disease associated with obstructive sleep apnea: just a coincidence?

Freitas, Luiz Antônio Rodrigues de “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-08-11T13:26:09Z No. of bitstreams: 1 Daltro C Nonalcoholic fatty....pdf: 166197 bytes, checksum: 303ec9aaa9e3f929cf67d00cf8986619 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-08-11T13:50:22Z (GMT) No. of bitstreams: 1 Daltro C Nonalcoholic fatty....pdf: 166197 bytes, checksum: 303ec9aaa9e3f929cf67d00cf8986619 (MD5)Made available in DSpace on 2017-08-11T13:50:22Z (GMT). No. of bitstreams: 1 Daltro C Nonalcoholic fatty....pdf: 166197 bytes, checksum: 303ec9aaa9e3f929cf67d00cf8986619 (MD5) Previous issue date: 2010Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB—BOL0554/2008).Bahiana School of Medicine and Public Health Salvador. Department of Internal Medicine. Salvador, BA, BrazilBahia Federal University of Medicine. Department of Internal Medicine Hepatology. Salvador, BA, BrazilObesity Treatment and Surgery Center. Salvador, BA, BrazilBahia Federal University of Medicine. Department of Pathology. Salvador, BA, BrazilBahia Federal University of Medicine. Department of Internal Medicine. Salvador, BA, BrazilBahiana School of Medicine and Public Health Salvador. Department of Internal Medicine. Salvador, BA, BrazilBahiana School of Medicine and Public Health Salvador. Department of Internal Medicine. Salvador, BA, BrazilBahiana School of Medicine and Public Health Salvador. Department of Internal Medicine. Salvador, BA, BrazilObesity is associated with obstructive sleep apnea (OSA) and nonalcoholic fatty liver disease (NAFLD). It has been shown that OSA could be an independent risk factor for NAFLD. OSA could cause not only insulin resistance but worse NAFLD through nocturnal hypoxemia. This study aimed to evaluate the frequency of OSA and NAFLD in obese patients and the relationship between OSA, insulin resistance, and severity of steatohepatitis (nonalcoholic steatohepatitis (NASH))

Nonalcoholic fatty liver disease in severely obese individuals: The influence of bariatric surgery

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-08-11T16:52:13Z No. of bitstreams: 1 Andrade AR Nonalcoholic fatty liver....pdf: 201925 bytes, checksum: 5453fe869ff0d42e8e05a230dc301598 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-08-11T17:11:12Z (GMT) No. of bitstreams: 1 Andrade AR Nonalcoholic fatty liver....pdf: 201925 bytes, checksum: 5453fe869ff0d42e8e05a230dc301598 (MD5)Made available in DSpace on 2017-08-11T17:11:12Z (GMT). No. of bitstreams: 1 Andrade AR Nonalcoholic fatty liver....pdf: 201925 bytes, checksum: 5453fe869ff0d42e8e05a230dc301598 (MD5) Previous issue date: 2009FAPESB (Fundação de Amparo a Pesquisa do Estado da Bahia), PRONEX - CNPqUniversidade Federal da Bahia. Postgraduate Course in Medicine and Health. Salvador, BA, BrasilUniversidade Federal da Bahia. Escola de Medicina. Salvador, BA, BrasilCenter for the Surgical Treatment of Obesity. Surgeon. Salvador, BA, BrasilUniversidade Federal da Bahia. Escola de Medicina. Salvador, BA, BrasilUniversidade Federal da Bahia. Programa de Pós Graduação em Medicina e Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Escola de Medicina. Salvador, BA, BrasilUniversidade Federal da Bahia. Escola de Medicina. Salvador, BA, BrasilUniversidade Federal da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilassociated with NAFLD, and bariatric surgery (BAS) is traditionally indicated for the treatment of severely obese individuals. Here, we discuss the behavior and prognosis of this liver disease following post-surgical weight loss. Aim: To evaluate the influence of the BAS on the clinical and biochemical parameters of NAFLD in severely obese patients. Methodology: An intervention study included obese individuals (BMI≥ 35kg/m2), who had been submitted to liver biopsy during BAS and had NAFLD. HAIR (hypertension, ALT and insulin resistance and BAAT (BMI, ALT, age and triglycerides) scores and FLI (Fatty Liver Index) were used to compare the patients at the time of surgery, and 12-30 months following weight loss. Results: From October 2004 to September 2007, 122 patients were diagnosed with NAFLD, 40 of whom agreed to participate in the study. The mean age was 37.7 ± 12.5 years, 60% were women and 80% had steatohepatitis (NASH) with fibrosis upon analysis of the liver biopsy performed during BAS. Mean weight loss was 46.0 ± 2.0 kg. After 21 ± 5.8 months of follow-up, a significant improvement was found in all the variables analyzed (79.3% according to the HAIR scores, 95.2% as measured by the BAAT score and 72.5% by the FLI. Conclusion: The results suggest that treatment of obesity by bariatric surgery may influence the prognosis of NAFLD. In addition to weight loss, we observed improvement in the clinical and biochemical parameters related to NAFLD, such as anthropometrics index, hypertension, aminotransferases, triglycerides and insulin resistance

Fatty liver disease in severe obese patients: Diagnostic value of abdominal ultrasound

AIM: To evaluate the sensitivity and specificity of abdominal ultrasound (US) for the diagnosis of hepatic steatosis in severe obese subjects and its relation to histological grade of steatosis

Doença hepática gordurosa não alcoólica: caractéristicas clínicas e histológicas em obesos graves submetidos à cirurgia bariátrica

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-06-11T19:14:00Z No. of bitstreams: 1 Bitencourt AGV Doença hepática....pdf: 326246 bytes, checksum: 18720a4140732c4a6efff7a45af64f07 (MD5)Made available in DSpace on 2014-06-11T19:14:00Z (GMT). No. of bitstreams: 1 Bitencourt AGV Doença hepática....pdf: 326246 bytes, checksum: 18720a4140732c4a6efff7a45af64f07 (MD5) Previous issue date: 2007Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilHospital Português. Núcleo de Cirurgia da Obesidade. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilObesidade é considerada um importante fator de risco da Doença Hepática Gordurosa Não Alcoólica (DHGNA), e um significante índice de gravidade da doença. Essa condição vem despertando interesse no estudo de obesos que se submetem à cirurgia bariátrica. Objetivo: Avaliar características clínicas e histológicas da DHGNA nestes indivíduos. Metodologia: O estudo incluiu 141 obesos (IMC≥ 35 kg/m2) submetidos à cirurgia bariátrica de outubro/2004 a maio/2005 tiveram diagnóstico de DHGNA. Foram excluídos os indivíduos com história de ingestão alcoólica (≥ 140g/semana) e portadores de outras doenças hepáticas. Os pacientes foram estudados através de parâmetros clínicos e histológicos. A biópsia hepática foi o primeiro procedimento na cirurgia, e a DHGNA foi graduada em esteatose isolada, esteatohepatite (esteatose com balonização ou fibrose) e cirrose. Resultados: A média de idade dos pacientes foi de 37±11 anos, sendo 76,4% eram do gênero feminino. Obesidade grau III (IMC> 40 kg/m2) esteve presente em 77,7% (141) dos casos, e síndrome metabólica foi observada em 52% (104). Elevação de aminotransferases esteve presente em 30% (31/105) dos casos, e 59% (105) apresentavam esteatose na ultra-sonografia. A biópsia mostrou fígado normal ou alterações inespecíficas em 11,3% dos casos e DHGNA em 88,7%: esteatose em 2,8%, esteato-hepatite em 85,1% (esteato-hepatite com fibrose em 76,6%), e cirrose em 0,7%. Conclusões: Em obesos graves a DHGNA é em geral assintomática e pode não apresentar alterações bioquímicas e ultrassonográficas. Entretanto, apresenta um amplo espectro histológico, com maior freqüência de casos de esteato-hepatite com fibrose. Os resultados sugerem que a biópsia hepática deve ser considerada durante cirurgia bariátrica visando uma melhor orientação destes pacientes.UNLABELLED: Obesity is an important risk factor of Nonalcoholic Fatty Liver Disease (NAFLD) and it has been considered a predictor of severity of this condition. However, in severe obese patients the spectrum of NAFLD is in discussion. AIM: To describe the clinical and histological characteristics of NAFLD in obese patients submitted to bariatric surgery. METHODOLOGY: The study included 141 severe obese (BMI > or = 35kg/m2) who underwent bariatric surgery from October 2004 to May 2005 had clinical and histological diagnosis of NAFLD. Exclusion criteria: alcoholic intake more than 140 g/week, positive markers for other liver diseases. All patients were submitted to hepatic biopsy, as first procedure during the surgery. RESULTS: The mean age of the patients was of 37.3 +/- 11.1 years and 76.4% were female. BMI was greater than 40 kg/m2 in 77.7% of the cases. The frequency of metabolic syndrome was 51.9% (104). Elevated aminotransferases and hepatic steatosis on ultrasound were present in 29.5% (105) and 59.0% (105) respectively. Normal liver biopsy or unspecfic findings were found in 11.3% of the cases and NAFLD in 88.7%: isolated steatosis in 2.8%, steatohepatitis (NASH) in 85.1% (NASH and fibrosis in 76.6%) and cirrhosis in 0.7%. CONCLUSIONS: NAFLD in severe obese patients, in general, was asymptomatic and presented normal enzymes, liver function and ultrasound. However, it has a large histological spectrum and cases of steatohepatitis with fibrosis were more frequent. These results suggest that liver biopsy may be considered during the bariatric surgery to better diagnosis and prognosis of severe obese patients