5 research outputs found
Pulmonary embolism in patients with severe COVID-19 treated with intermediate- to full-dose enoxaparin: A retrospective study
Coronavirus disease (COVID-19) may predispose patients to pulmonary embolism (PE), despite standard thromboprophylaxis. Our retrospective study aimed to report the prevalence of PE in patients with COVID-19 and severe respiratory failure (SRF) treated with intermediate- to full-dose enoxaparin. We analyzed data from patients with COVID-19 pneumonia and SRF admitted to our Respiratory Intensive Care Unit (RICU) from February 27 to April 20, 2020. All patients received at least intermediate-dose enoxaparin (40 mg twice daily). Computed tomography pulmonary angiography (CTPA) was used to detect PE. Ninety-two patients with COVID-19 pneumonia and SRF were admitted to our RICU. Twenty-two patients underwent CTPA (24 %), 11 of whom had PEs (12%). We hypothesize that the enoxaparin treatment may be responsible for the lower prevalence of PE as compared to previous reports of similar patients, even if our report had several limitations, mainly the small sample size
Influence of the Learning Effect on the Diagnostic Yield of Endobronchial Ultrasound-guided Transbronchial Needle Aspiration of Mediastinal and Hilar Lymph Nodes
The diagnostic yield of conventional
transbronchial needle aspiration (TBNA) is characterized
by a learning effect. The aim of this retrospective
study was to verify whether a learning curve
similarly affected the yield of endobronchial
ultrasound-guided (EBUS)-TBNA. To this end, we
evaluated the sensitivity and diagnostic accuracy of
EBUS-TBNA during the first 3 years of activity.
Methods: EBUS-TBNA was performed by 2 operators
with no previous experience in this technique. Cytologic
samples were obtained from mediastinal and hilar
lymph nodes enlarged at a chest computed tomography
scan and/or with increased fluorodeoxyglucose uptake
at computed tomography/positron emission tomography
scan in patients with suspected lung cancer. The
cytologic diagnosis of EBUS-TBNA samples has been
compared with the final diagnosis obtained from further
diagnostic procedures, surgery, or clinical-radiologic
follow-up.
Results: From October 2012 to October 2015, we
collected 408 EBUS-TBNA cytologic samples from
313 patients: 223 samples were positive for metastatic
involvement and 185 were nonmetastatic. The latter
included 137 true-negative and 48 false-negative
results. The final diagnosis comprised 271
metastatic and 137 nonmetastatic lymph nodes.
The overall sensitivity for cancer was 82% and
diagnostic accuracy was 88%. Sensitivity and accuracy
per year were as follows: first year, 78% and
82% in 90 nodal samples; second year, 83% and 89%
in 144 nodal samples; third year, 85% and 91% in
174 nodal samples.
Conclusions: EBUS-TBNA can be considered as a reliable
tool even if performed by operators without
previous experience in this procedure, and the diagnostic
yield continues to increase progressively over a
long time
Variant of Concern-Matched COVID-19 Convalescent Plasma Usage in Seronegative Hospitalized Patients
COVID-19 convalescent plasma (CCP) has been the only specific anti-viral therapy against SARS-CoV-2 available for more than one year. Following the negative results from most randomized controlled trials on its efficacy in COVID-19 hospitalized patients and the availability of anti-spike monoclonal antibodies (mAbs), the use of CCP has subsequently rapidly faded. However, the continuous appearance of new variants of concern (VOCs), most of which escape mAbs and vaccine-elicited neutralizing antibodies (nAbs), has renewed the interest towards CCP, at least in seronegative immunocompetent patients, and in immunocompromised patients not able to mount a protective immune response. We report here the experience of a single Italian hospital in collecting and transfusing CCP in immunocompromised patients hospitalized for severe COVID-19 between October 2021 and March 2022. During this 6-month period, we collected CCP from 32 vaccinated and convalescent regular blood donors, and infused high nAb-titer CCP units (titered against the specific VOC affecting the recipient) to 21 hospitalized patients with severe COVID-19, all of them seronegative at the time of CCP transfusion. Patients’ median age was 66 years (IQR 50–74 years) and approximately half of them (47.6%, 10/21) were immunocompromised. Two patients were rescued after previous failure of mAbs. No adverse reactions following CCP transfusion were recorded. A 28-day mortality rate of 14.3 percent (3/21) was reported, with age, advanced disease stage and late CCP transfusion associated with a worse outcome. This real-life experience also supports the use of CCP in seronegative hospitalized COVID-19 patients during the Delta and Omicron waves
Molecular Testing in EBUS-TBNA Specimens of Lung Adenocarcinoma: A Study of Concordance Between Cell Block Method and Liquid-Based Cytology in Appraising Sample Cellularity and EGFR Mutations
Purpose Cytological endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) specimens of mediastinal
lymph node metastasis are frequently used to perform concomitant diagnosis, staging and genetic testing in non-smallcell
lung cancer (NSCLC). The purposes of this single-center retrospective study were to evaluate EBUS-TBNA samples\u2019
adequacy for molecular testing of epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK), and to
analyze the concordance between the cell block method and liquid-based cytology (LBC) in appraising the sample cellularity
and in detecting EGFR mutation.
Materials and Methods We retrospectively examined 82 patients who underwent EBUS-TBNA from October 2012 to September
2015 and received a confirmed diagnosis of lymph node metastasis of lung adenocarcinoma. Each sample was
processed using both cell block and LBC to carry out DNA analysis (adequacy criterion: tumor cell percentage > 25%) and
EGFR mutation testing.
Results Fifty-four patients were male, 66 were current or former-smokers, and the median age was 67 years. The median
size of sampled lymph nodes was 14.8 mm. Seventy-one and 66 samples were adequate to perform cell block and LBC,
respectively. The \u3ba-statistic (0.78) showed an excellent concordance. EGFR mutation was detected in eight patients using cell
block and in seven using LBC, with a simple percentage agreement of 87.5%. ALK translocation was found in two patients.
Conclusions This study demonstrates the feasibility of EGFR mutation analysis with both cell block and LBC, with an excellent
concordance between the two methods. Considering that the majority of advanced NSCLCs are diagnosed on cytology
specimens, LBC is feasible and needs to be implemented for ancillary tests (immunocytochemistry, molecular analysis)
Convalescent Plasma for Hospitalized COVID-19 Patients: A Single-Center Experience
In Winter 2020, Italy, and in particular the Lombardy region, was the first country in the Western hemisphere to be hit by the COVID-19 pandemic. Plasma from individuals recovered from COVID-19 (COVID-19 convalescent plasma, CCP) was the first therapeutic tool adopted to counteract the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). In this retrospective cohort study, we report the experience of the city hospital of Mantua, Lombardy region, on the compassionate use of CCP in patients hospitalized for severe COVID-19. Between April 2020 and April 2021, 405 consecutive COVID-19 patients received 657 CCP units with a median anti-SARS-CoV-2 neutralizing antibody (nAb) titer of 160 (interquartile range (IQR), 80–320). Their median age was 68 years (IQR, 56–78 years), and 62% were males. At enrollment, 55% of patients had an increased body mass index (BMI), and 25.6% had at least three comorbidities. The 28-day crude mortality rate was 12.6% (51/405). Young age (<68 years), mild disease (admission to low-intensity departments) and early treatment (<7 days from symptoms onset) with high nAb titer (≥320) CCP were found as independently associated with a favorable response to CCP treatment. No safety concerns were recorded, with a rate of CCP-related adverse reactions (all of mild intensity) of 1.3%. In our real-life experience, the first in the western world, early administration of high-titer CCP was a safe and effective treatment for hospitalized COVID-19 patients