The Greening of Anthocyanins: Eco-Friendly Techniques for Their Recovery from Agri-Food By-Products

In recent years, several steps forward have been made toward a more sustainable approach for the extraction of bioactive compounds from plant materials based on the application of green extraction principles. It is currently recognized that waste and by-products deriving from agriculture and food industries still contain a wide array of high value-added substances, which can be re-used to obtain new products with various applications in the food, supplement, pharmaceutical, and cosmetic industries. Anthocyanins are a class of these valuable metabolites; they confer the red, violet, and blue color to fruits and vegetables, and scientific evidence has accumulated over the last few decades to support their beneficial effects on human health, in great part deriving from their powerful antioxidant capacity. This review provides a general overview of the most recent green procedures that have been applied for the recovery of anthocyanins from plant-derived wastes and by-products. The most widely used green solvents and the main sustainable techniques utilized for recovering this class of flavonoids from various matrices are discussed, together with the variables that mainly impact the extraction yield

Extracţia fenolilor şi flavonoidelor din specia Centaurea CyanuS L

Obiectivul prezentei lucrări a fost de a obţine extracte polifenolice din Centaurea cyanus L. În vederea optimizării condiţiilor extracţiei s-au analizat parametrii care pot influenţa procesul: tipul şi concentraţia solventului (etanol 60 şi 80%), produsul vegetal, perioada de colectare a produsului vegetal, numărul extracţiilor. S-a demonstrat că totalul compuşilor fenolici şi flavonoidelor este maximum în extractul hidroalcoolic 60% (350.91 mgGAE/g şi 274.28 mg rutozidă/g masă uscată, respectiv), obţinut din părţi aeriene colectate la începutul înfloririi

Characterization and Biological Activities of In Vitro Digested Olive Pomace Polyphenols Evaluated on Ex Vivo Human Immune Blood Cells

Olive pomace (OP) represents one of the main by-products of olive oil production, which still contains high quantities of health-promoting bioactive compounds. In the present study, three batches of sun-dried OP were characterized for their profile in phenolic compounds (by HPLC-DAD) and in vitro antioxidant properties (ABTS, FRAP and DPPH assays) before (methanolic extracts) and after (aqueous extracts) their simulated in vitro digestion and dialysis. Phenolic profiles, and, accordingly, the antioxidant activities, showed significant differences among the three OP batches, and most compounds showed good bioaccessibility after simulated digestion. Based on these preliminary screenings, the best OP aqueous extract (OP-W) was further characterized for its peptide composition and subdivided into seven fractions (OP-F). The most promising OP-F (characterized for its metabolome) and OP-W samples were then assessed for their potential anti-inflammatory properties in ex vivo human peripheral mononuclear cells (PBMCs) triggered or not with lipopolysaccharide (LPS). The levels of 16 pro-and anti-inflammatory cytokines were measured in PBMC culture media by multiplex ELISA assay, whereas the gene expressions of interleukin-6 (IL-6), IL-10 and TNF-α were measured by real time RT-qPCR. Interestingly, OP-W and PO-F samples had a similar effect in reducing the expressions of IL-6 and TNF-α, but only OP-W was able to reduce the release of these inflammatory mediators, suggesting that the anti-inflammatory activity of OP-W is different from that of OP-F

Clinopodium tomentosum (Kunth) Govaerts Leaf Extract Influences in vitro Cell Proliferation and Angiogenesis on Primary Cultures of Porcine Aortic Endothelial Cells

Clinopodium tomentosum (Kunth) Govaerts is an endemic species in Ecuador, where it is used as an anti-inflammatory plant to treat respiratory and digestive affections. In this work, effects of a Clinopodium tomentosum ethanolic extract (CTEE), prepared from aerial parts of the plant, were investigated on vascular endothelium functions. In particularly, angiogenesis activity was evaluated, using primary cultures of porcine aortic endothelial cells (pAECs). Cells were cultured for 24 h in the presence of CTEE different concentrations (10, 25, 50, and 100 \u3bcg/ml); no viability alterations were found in the 10-50 \u3bcg/ml range, while a slight, but significant, proliferative effect was observed at the highest dose. In addition, treatment with CTEE was able to rescue LPS-induced injury in terms of cell viability. The CTEE ability to affect angiogenesis was evaluated by scratch test analysis and by an in vitro capillary-like network assay. Treatment with 25-50\u3bcg/ml of extract caused a significant increase in pAEC\u2019s migration and tube formation capabilities compared to untreated cells, as results from the increased master junctions\u2019 number. On the other hand, CTEE at 100 \u3bcg/ml did not induce the same effects. Quantitative PCR data demonstrated that FLK-1 mRNA expression significantly increased at a CTEE dose of 25\u3bcg/ml. The CTEE phytochemical composition was assessed through HPLC-DAD; rosmarinic acid among phenolic acids and hesperidin among flavonoids were found as major phenolic components. Total phenolic content and total flavonoid content assays showed that flavonoids are the most abundant class of polyphenols. The CTEE antioxidant activity was also showed by means of the DPPH and ORAC assays. Results indicate that CTEE possesses an angiogenic capacity in a dose-dependent manner; this represents an initial step in elucidating the mechanism of the therapeutic use of the plant