Parliamentary Scrutiny of Legislation under the Human Rights Act 1998

The enactment of the Human Rights Act 1998 in the United Kingdom has widely affected many aspects of the operation of Government, including the traditional separation of judiciary, legislature, and executive. This paper examines the scope and process adopted for the scrutiny of legislation within the framework of the Human Rights Act 1998. The particular focus canvasses the first year's operation of the Joint Committee on Human Rights and its role as watchdog

Wall Adhesion and Constitutive Modelling of Strong Colloidal Gels

Wall adhesion effects during batch sedimentation of strongly flocculated colloidal gels are commonly assumed to be negligible. In this study in-situ measurements of colloidal gel rheology and solids volume fraction distribution suggest the contrary, where significant wall adhesion effects are observed in a 110mm diameter settling column. We develop and validate a mathematical model for the equilibrium stress state in the presence of wall adhesion under both viscoplastic and viscoelastic constitutive models. These formulations highlight fundamental issues regarding the constitutive modeling of colloidal gels, specifically the relative utility and validity of viscoplastic and viscoelastic rheological models under arbitrary tensorial loadings. The developed model is validated against experimental data, which points toward a novel method to estimate the shear and compressive yield strength of strongly flocculated colloidal gels from a series of equilibrium solids volume fraction profiles over various column widths.Comment: 37 pages, 12 figures, submitted to Journal of Rheolog

Expression and Circular Dichroism Studies of the Extracellular Domain of the alpha Subunit of the Nicotinic Acetylcholine Receptor

To provide material suitable for structural studies of the nicotinic acetylcholine receptor, we have expressed and purified the NH2-terminal extracellular domain of the mouse muscle alpha subunit. Several constructs were initially investigated using Xenopus oocytes as a convenient small scale expression system. A fusion protein (alpha210GPI) consisting of the 210 NH2-terminal amino acids of the alpha subunit and a glycosylphosphatidylinositol anchorage sequence conferred surface alpha-bungarotoxin binding in oocytes. Coexpression of alpha210GPI with an analogous construct made from the delta subunit showed no evidence of heterodimer formation. The alpha210GPI protein was chosen for large scale expression in transfected Chinese hamster ovary cells. The alpha210GPI protein was cleaved from these cells and purified on an immunoaffinity column. Gel and column chromatography show that the purified protein is processed as expected and exists as a monomer. The purified protein also retains the two distinct, conformation-specific binding sites expected for the correctly folded alpha subunit. Circular dichroism studies of alpha210GPI suggest that this region of the receptor includes considerable beta-sheet secondary structure, with a small proportion of alpha-helix

Green Infrastructure Training for Veterans

The Environmental Protection Agency launched a Strategic Agenda to Protect Waters and Build More Livable Communities through Green Infrastructure in April of 2011. This initiative supports local communities in using green infrastructure to manage rain water while creating co-benefits of green space, which makes communities more attractive and livable. (EPA) In the fall of 2014 the Environmental Protection Agency along with the U.S. Department of Agriculture, U.S. Department of Defense, U.S. Department of Energy, U.S. Department of Housing and Urban Development, U.S. Department of the Interior and U.S. Department of Transportation signed a federal letter of support for green infrastructure recognizing it as an important tool for building resilience to climate change impacts such as increased heavy rainfall and heat island effect. Each agency committed to taking specific action to promote green infrastructure including but not limited to: creating alliances, providing community assistance, funding, training, development of metrics to assess performance of green infrastructure, and promotion best practices. (Federal) The City of XXXX, the Green Infrastructure Center, Inc., and X University have collaborated on a National Fish and Wildlife Federation Grant titled Developing a Green Infrastructure Plan and Network for the X River Network . As part of the project X University will provide a training program to prepare Veterans for the green jobs of the future. Green infrastructure projects can provide business and employment opportunities for veterans. This project includes a 20 hour educational course for veterans to educate them about the green infrastructure industry, business and employment opportunities in green infrastructure and green infrastructure storm water management techniques. Hands-on training will be incorporated in the course work. This program will develop a base level certification that will qualify recipients for more advanced and specialized programs. This green infrastructure training certificate will be coordinated with other green infrastructure education certifications and programs in a stackable manner to provide a transparent track for career advancement, which will benefit employers and the green infrastructure industry. This paper will highlight pre- and post training survey results and provide insight into the progress of the veterans transitioning to this employment area

Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media

This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19

The impact of patient participation direct enhanced service on patient reference groups in primary care: a qualitative study.

NHS policy documents continue to make a wide-ranging commitment to patient involvement. The Patient Participation Direct Enhanced Service (PP-DES), launched in 2011, aimed to ensure patients are involved in decisions about the range and quality of services provided and commissioned by their practice through patient reference groups (PRGs). The aim of this exploratory study is to review the impact of the PP-DES (2011-13) on a sample of PRGs and assess how far it has facilitated their involvement in decisions about the services of their general practices.The National Institute for Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care (CLAHRC) for Leicestershire, Northamptonshire and Rutland (LNR)

Sensory protein kinase signalling in ' Schistosoma mansoni ' Cercariae : host location and invasion

Schistosoma mansoni cercariae display specific behavioural responses to abiotic/biotic stimuli enabling them to locate and infect the definitive human host. Here we report the effect of such stimulants on signalling pathways of cercariae in relation to host finding and invasion. Cercariae exposed to various light/temperature regimes displayed modulated protein kinase C (PKC), extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38 MAPK) activities, with distinct responses at 37°C and intense light/dark, when compared to 24°C under normal light. Kinase activities were localized to regions including the oral sensory papillae, acetabular ducts, tegument, acetabular glands, and nervous system. Furthermore, linoleic acid (LA) modulated PKC and ERK activities concurrent with the temporal release of acetabular gland components. Attenuation of PKC, ERK and p38 MAPK activities significantly reduced gland component release, particularly in response to LA, demonstrating the importance of these signalling pathways to host penetration mechanisms