Statistical reconstruction of three-dimensional porous media from two-dimensional images

A method of modelling the three-dimensional microstructure of random isotropic two-phase materials is proposed. The information required to implement the technique can be obtained from two-dimensional images of the microstructure. The reconstructed models share two-point correlation and chord-distribution functions with the original composite. The method is designed to produce models for computationally and theoretically predicting the effective macroscopic properties of random materials (such as electrical and thermal conductivity, permeability and elastic moduli). To test the method we reconstruct the morphology and predict the conductivity of the well known overlapping sphere model. The results are in very good agreement with data for the original model.Comment: 11 pages, 12 figure

The cloning, characterisation and engineering of an IGF-I-BINDING single chain Fv

This thesis describes the construction and characterisation of an insulin-like growth factor (IGF-I)-binding single chain Fv (scFv) and the utilisation of this scFv as a model protein for the study of the application of DNA shuffling and ribosome display to antibody engineering. The variable domain genes were isolated from the hybridoma cell line producing the monoclonal antibody and successfully joined by PCR for the construction of the scFv, named anti-GPE. Sequencing of the gene revealed an unusually short heavy chain CDR2 region. The cloned scFv was expressed in E. coli and purified. Expression levels were low and the protein has poor solubility, most likely due to a reduction in folding efficiency caused by the abbreviated CDR2. The purified monomeric form of the protein was analysed for binding to IGF-I using surface plasmon resonance on the BIAcore 1000 with the specificity of the IgG version of the antibody for the three N-terminal residues of IGF-I - Gly-Pro-Glu - reproduced. The scFv's calculated dissociation constant of 3.68 µM is a low affinity for an antibody and is approximately 36-fold weaker than was calculated for the Fab version of the antibody, but it is concluded that the calculated affinity for the scFv was an apparent affinity that may be an underestimation of true affinity due to the presence of non-functional or misfolded scFv species within the gel-filtration purified monomer peaks. A mutant version of anti-GPE with residues inserted in the CDR2 to restore it to normal length produced a protein with improved expression and solubility characteristics while retaining IGF-I-binding. It was concluded that the short CDR2 was due to deletions generated during the somatic mutation process and a model for this is described. A ribosome display method using a rabbit reticulocyte lysate as a source of ribosomes was developed for specific selection of anti-GPE against IGF-I. Error prone PCR was used to produce a random point mutated library of anti-GPE (EPGPE). This was taken through several cycles of display and selection but selection for non-specifically binding scFvs was commonly observed. This was probably due to poor folding of ribosome-displayed proteins in the system used, possibly caused by the presence of DTT in the lysate and/or the low capacity of the anti-GPE framework to tolerate mutation while retaining stability. It is assumed misfolds, exposing hydrophobic regions, would have a tendency to non-specifically interact with the selection surface. Of the 64 EPGPE clones screened from four rounds of display and selection, many were shown to have poor or non-specific binding, but one scFv was characterised that was affinity matured 2.6-fold over anti-GPE wild type affinity for IGF-I. A DNA shuffling method was developed to produce libraries of chimaeric scFvs between anti-GPE and NC10 (anti-neuraminidase scFv) with the objective of isolating functional IGF-I-binding chimaeras. The NC10 scFv had its CDRs replaced with the anti-GPE CDRs prior to the shuffling to increase the likelihood of isolating IGF-I binders. Ribosome display was used for selection from the chimaera libraries. Selection strategies included elution of specific binders by GPE peptide and a GPE 10-mer peptide. Selection was also performed using IGF-I immobilised on a BIAcore sensorchip as a selection surface. Again, much non-specific selection was observed as seen for display of EPGPE, for what was expected to be the same reasons. Selected scFvs were genuinely chimaeric but with poor expression and solubility and mostly non-specific in their binding. One characterised selected chimaera, made up of three segments of each of the parental scFvs, was shown to bind specifically to IGF-I by BIAcore. Steps to improve the efficiency of the ribosome display system have been identified and are discussed

Diffusion approximation for self-similarity of stochastic advection in Burgers' equation

Self-similarity of Burgers' equation with some stochastic advection is studied. In self-similar variables a stationary solution is constructed which establishes the existence of a stochastically self-similar solution for the stochastic Burgers' equation. The analysis assumes that the stochastic coefficient of advection is transformed to a white noise in the self-similar variables. Furthermore, by a diffusion approximation, the long time convergence to the self-similar solution is proved in the sense of distribution.Comment: 37 pages, Comm. Math. Phys., to appear, 201

Sources of Chinese Demand for Resource Commodities

Is China’s demand for resources driven predominantly by domestic factors or by global demand for its exports? The answer to this question is important for many resource-exporting countries, such as Australia, Brazil, Canada and India. This paper provides evidence that China’s (mainly manufacturing) exports have been a significant driver of its demand for resource commodities over recent decades. First, it employs input-output tables to demonstrate that, historically, manufacturing has been at least as important as construction as a driver of China’s demand for resource-intensive metal products. Second, it shows that global trade in non-oil resource commodities can be described by the gravity model of trade. Using this model it is found that, controlling for domestic expenditure (including investment), exports are a sizeable and significant determinant of a country’s resource imports, and that this has been true for China as well as for other countries.China; trade; investment; resource commodities; gravity model

China's Steel Industry

China's steel industry has grown rapidly in recent decades, with China now the world's largest producer and consumer of steel. This has resulted in a sharp increase in demand for iron ore and coal, Australia's two largest exports, which are key inputs for the steelmaking process. This article discusses the growth of the Chinese steel industry over the past couple of decades.China; steel industry; Chinese steel industry; iron ore; coking coal

The introduction of space technology power systems into developing countries

Between 1978 and 1984, NASA-Lewis was responsible for the design, fabrication, installation and operational support of 57 photovoltaic power systems in 27 countries. These systems were installed in locations not served by a central power system and ranged in size from 40 W for powering street lights to 29 kW for providing power to a complete village. Several of the system projects had socio/economic studies components that provided for an assessment of how the introduction of both electricity and a novel high technology power system affected the users and their society