Standardization process by NAA method for phytopharmaceutical industry

The ancient therapeutic remedies for modern needs are represented by medicinal crop plants, the raw material for phytopharmaceutical forms. Medicinal plants are generally viewed only in terms of beneficial effects without considering the potentially toxic side [1]. In this context in the present study elemental composition for seven representative medicinal crop plants as: coriander, dill, Echinacea, lavender, chamomile, mint and plantain, cultivated in unpolluted areas in Romania, were analyzed. Among many analytical methods used for determination of plant elemental content, that generally imply the vegetal matrix disintegration, the Neutronic Activation Analysis (NAA) is classified as primary ratio method [2]. By validating the NAA procedure by statistical evaluation of the nominal error En≤1 (ISO 13528-2015) [3], NAA proved to be an accurate, specific and multielement analysis technique for medicinal plants with the advantage of eliminating the preliminary step of digestion, in this way certain errors being avoided. This method is differentiated as one with high potential to obtain internal plant standards in the phytopharmaceutical industry. These standards can be vegetable matrix, or plant specific, once achieved can be used a long period of time to verify and validate more routinely accessible analytical techniques, which in turn require a digestion stage. A critical assessment provided by the results include the essential nutrients (K, Ca, Mg, Na, Fe, K, Mn, Zn), micro- and trace elements (Co, Cr, Cu, Ni, Se, V), as well as the undesirable, potentially toxic elements (Al, As, Ba, Co, Cr, Ni, Sb) together with rare earth elements. The values are comparable with literature. These plants may provide a useful contribution to food intake with essential macronutrients (K, Ca, Mg) and the concerns regarding the toxicity of metals for a person, are removed. Seemingly the Al (4997 ppm) and Fe (3315 ppm) [4] content in lavender is of some worry. In fact, many studies [5] revealed that some plants species, and lavender among them, exhibit metal bioaccumulation properties and are used for phytoremediation of contaminated soils. This dual implication, beneficial for soil depollution, but critical in transmitting the accumulated metals to humans, is to be considered. Choosing an unpolluted area, in order to cultivate medicinal plants, seems to be an efficient strategy from this point of view. Still, another conclusion is that a rigorous analytical control of the raw materials is advisable for some of the elements (among them aluminium) to avoid risks

An Update On Natural Compounds and Their Modern Formulations for the Management of Malignant Melanoma

The chapter includes a brief presentation of the types of skin cancer. The most aggressive type of skin cancer, melanoma, is discussed from the point of view of incidence, molecular, and immunohistochemical mechanism along with the most important biomarkers for identification. Recent studies containing active phytocompounds with chemopreventive activity pointing toward phytochemicals used for melanoma prevention and therapy are reviewed. Modern physicochemical formulations for the enhancement of bioavailability of some active phytochemicals with chemopreventive activity for malignant melanoma are discussed

New Insights Regarding the Potential Health Benefits of Isoflavones

Isoflavones are a class of plant secondary metabolites, with an estrogen‐like structure presenting a plethora of biological activities. The chapter discusses important facts about this class of phytoestrogens, from biosynthesis to the latest research about their health benefits. The following major points discussed are: biosynthesis, regulation, isolation, metabolism and bioavailability, isoflavones in diet and intake, and new insights regarding the therapeutic effect including cancer chemoprevention. The chapter ends with a mini review of own research of the anti‐inflammatory and chemopreventive activity of isoflavonoid genistein alone and incorporated in modern pharmaceutical formulations. The chapter updates the interested researchers in the field with the latest progress regarding potential health benefits of isoflavones

Cardioprotective Effects of Cultivated Black Chokeberries (<em>Aronia</em> spp.): Traditional Uses, Phytochemistry and Therapeutic Effects

Cardiovascular diseases represent the main cause of morbidity and mortality worldwide. Obesity, sedentary life style, diet, smoking and stress are the principal inducers of hypertension, endothelium dysfunction and insulin resistance in the developed countries. The latest in vitro and in vivo studies on different type of extracts obtained from black-fruited Aronia highlight its excellent cardioprotective actions for the prevention and treatment of cardiovascular and metabolic disorders. So, this chapter aims to bring an up-to-date regarding the antioxidant, anti-inflammatory, anti-atherosclerotic, antiplatelet, blood pressure, glucose and lipid reduction properties of black-fruited Aronia, as a possible new therapeutic strategy for the primary and secondary prevention of cardiovascular pathologies

Effects of Quercetin on Blood Pressure: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

BACKGROUND: Quercetin, the most abundant dietary flavonol, has antioxidant effects in cardiovascular disease, but the evidence regarding its effects on blood pressure (BP) has not been conclusive. We assessed the impact of quercetin on BP through a systematic review and meta-analysis of available randomized controlled trials. METHODS AND RESULTS: We searched PUBMED, Cochrane Library, Scopus, and EMBASE up to January 31, 2015 to identify placebo-controlled randomized controlled trials investigating the effect of quercetin on BP. Meta-analysis was performed using either a fixed-effects or random-effect model according to I(2) statistic. Effect size was expressed as weighted mean difference (WMD) and 95% CI. Overall, the impact of quercetin on BP was reported in 7 trials comprising 9 treatment arms (587 patients). The results of the meta-analysis showed significant reductions both in systolic BP (WMD: -3.04 mm Hg, 95% CI: -5.75, -0.33, P=0.028) and diastolic BP (WMD: -2.63 mm Hg, 95% CI: -3.26, -2.01, P CONCLUSIONS: The results of the meta-analysis showed a statistically significant effect of quercetin supplementation in the reduction of BP, possibly limited to, or greater with dosages of \u3e500 mg/day. Further studies are necessary to investigate the clinical relevance of these results and the possibility of quercetin application as an add-on to antihypertensive therapy

Lupan-Skeleton Pentacyclic Triterpenes with Activity against Skin Cancer: Preclinical Trials Evolution

Skin cancer is an increasingly frequent pathology, with a dangerous high percentage of malignant melanoma. The use of synthetic chemotherapy raises the problem of severe adverse effects and the development of resistance to treatment. Therefore, the use of natural therapies became the focus of numerous research groups due to their high efficacy and lower systemic adverse effects. Among natural products evaluated as therapeutical agents against skin cancer, betulinic acid was emphasized as a highly selective anti-melanoma agent and is currently undergoing phase II clinical trials as topical application. Several other pentacyclic triterpenes exhibit antiproliferative activities. This chapter aims to present the latest main discoveries in the class of pentacyclic triterenes with antitumor effect and the evolution of their preclinical trials. Furthermore, it includes reports on plant sources containing pentacyclic triterpenes, as well as the main possibilities of their water solubilization and cancer cell targeting. A review on recent data regarding mechanisms of action at cellular and molecular levels complements information on the outstanding medicinal potential of these compounds

Potential Barriers of Patient Involvement in Health Technology Assessment in Central and Eastern European Countries

Patients' perspectives are important to identify preferences, estimate values and appreciate unmet medical needs in the process of research and development and subsequent assessment of new health technologies. Patient and public involvement in health technology assessment (HTA) is essential in understanding and assessing wider implications of coverage and reimbursement decisions for patients, their relatives, caregivers, and the general population. There are two approaches to incorporating the patients' voice in HTA, preferably used in a mix. In the first one, patients, caregivers and/or their representatives directly participate at discussions in different stages of the HTA process, often at the same table with other stakeholders. Secondly, patient involvement activities can be supported by evidence on patient value and experience collected directly from patients, caregivers and/or their representatives often by patient groups Patient involvement practices, however, are limited in Central and Eastern European (CEE) countries without clear methodology or regulatory mechanisms to guide patient involvement in the HTA process. This poses the question of transferability of practices used in other countries, and might call for the development of new CEE-specific guidelines and methods. In this study we aim to map potential barriers of patient involvement in HTA in countries of the CEE region

Facilitation of Employability for Maternity and Parental Leavers in Europe: A Scoping Review

This report presents the findings of an European project concerning facilitation of employability for parental leavers in European countries. The conclusions and recommendations in this report build on comparisons of relevant European policy documents, country nates from five European countries (France, Hungary, the Netherlands, Norway and Slovakia) and a search in relevant databases. Taken together, these data form an approximate scoping review - to map a wide range of literature. Both the concepts of maternal and parental leave are used in the present report. Research related to leave in connection with young children use the term maternity leave until the last decades where more frequent parental leave is used. We are aware that a distinction between parental and maternity leave does not apply in same countries. A main finding is that parental leave and employability is a complex and context sensitive area. This relates to the fact that parental leave must be seen in connection with both individual, organisational, cultural, historical and political (legislative) issues on both national and European level. In this study, the focus has been mainly descriptive on the political (legislative) level, while a more interpretative analysis is made on how to develop tools and educational programs concerning development of employability skills relevant for parental leavers and employers. Analysis of the situation regarding parental leavers' employment in the partner countries shows that maternal employment in France, Slovakia and Hungary is rather low. Although, in Hungary there are networks which provide special counselling for women and support them when it comes to their (re)integration into the labour market. In Western Europe, such as the Netherlands and Norway, the situation is more favourable, employers are more flexible and part-time job is a frequent option offered. However, the up-to-date level of skills of parental leavers coming back to work may be a challenge in these countries too. The duration and generosity of paid parental leave seems to affect when (after what time) parents return to work. The period of leave cannot be too short, nor too long. The optimal length of the leave varies greatly depending on national conditions and cultural frameworks. In any case, it should not be so long that the parental leavers become uninteresting for the labour market and lose working competence (Kalb, 2018; Whitehouse, Romaniuk, Lucas & Nicholson, 2013).This project has been funded with support from the Erasmus+ Programme of the European Commission