Investigating Kinesin Kif18A as a Novel Therapeutic Target for Aggressive Cancers

Identifying potential therapeutic targets for aggressive cancers is critical to minimizing side effects of treatments and ultimately increasing patient treatment compliance. Here we demonstrate that kinesin motor protein Kif18A has the potential to serve as a minimally toxic target for the treatment of cancers such as triple negative breast cancer (TNBC) and colorectal cancer (CRC). Kif18A inhibition reduces the proliferation of multiple TNBC cell types and one CRC cell type. While endogenous Kif18A expression does not seem to indicate cell type specific sensitivity to Kif18A inhibition, the expression levels are higher in cancer cells than in normal somatic cells. Modal chromosome number has the strongest correlation with cell sensitivity to Kif18A depletion, indicating that Kif18A is heavily involved in the regulation of mitotic progression for cells with chromosomal instability. Furthermore, cells with chromosomal instability appear to arrest in mitosis and form multipolar spindles upon Kif18A knockdown. Based on these data, Kif18A may serve as a novel therapeutic target for aggressive, chromosomally unstable cancers because of its role in regulating mitotic spindle dynamics to promote successful progression through mitosis

Functional performance, depression, anxiety and stress in people with spinal cord injuries in Thailand: A Transition from hospital to home

The numbers of people with spinal cord injuries (SCI) in Thailand are on the increase. Thai traditional rehabilitation focuses its treatment in acute care with little attention to the lives of clients after their discharge from institutions. In addition to functional disability, emotional states of depression, anxiety, and stress are often involved in SCI. The purpose of this study is to identify and compare the outcomes of functional performance, depression, anxiety and stress of people with SCI, both at discharge and at post-discharge from hospital. It also identifies factors influencing these issues, for this group. The study participants were 121 people with SCI, recruited from ten major hospitals in Thailand. Data was collected at 48 hours pre-discharge and again at three months post-discharge, using the Functional Independence Measure (FIM) and the Depression, Anxiety, and Stress Scale (DASS). The results demonstrated that functional performance at discharge was significantly higher, than at three months post-discharge. Depression and anxiety at discharge were significantly lower than depression and anxiety at three months post-discharge. However, stress had not significantly changed from discharge to post-discharge. Factors influencing functional performance were marital status, number of architectural barriers, fulfilment of occupational therapy (OT) needs, and the number of service needs remaining unmet. Factors influencing depression, anxiety and stress were marital and economic status, education level, fulfilment of OT needs, and numbers of service needs remaining unmet. Rehabilitation professionals can eliminate these problems by bridging the gap of transition from hospital to home, for people with SCI

Optimisation multicritère pour la gestion de dépendances logicielles : utilisation de la norme de Tchebycheff

Session "Posters"National audienceLe problème de gestion de dépendances logicielles concerne l'installation d'applications informatiques modulaires. Il s'agit de programmes ayant la faculté d'être configurés par l'utilisateur, qui peut choisir à tout moment les modules qu'il souhaite installer ou enlever. Un module peut nécessiter la présence d'autres modules pour fonctionner, il peut entrer en conflit avec certains modules, et parfois il peut recommander l'installation de modules spécifiques pour pouvoir être utilisé au meilleur de ses capacités. Il existe généralement plusieurs solutions (listes de paquets à installer et à enlever) pour passer d'une configuration courante à une configuration souhaitée. On peut se donner des critères pour ordonner ces solutions, passant alors d'un problème de décision (" est-ce que je peux ajouter tous ces modules ? ") à un problème d'optimisation (" quelle est la meilleure solution pour ajouter tous ces modules ? "). Dans de nombreux cas, il faut prendre en compte plusieurs critères. Cet article se concentre sur des méthodes algorithmiques capables de calculer des solutions équilibrées en utilisant la norme de Tchebycheff comme méthode d'agrégation de critères. Cette approche est ensuite évaluée sur des problèmes de gestion de dépendances entre paquets GNU/Linux

Antiplatelet therapy in atherothrombotic diseases : similarities and differences across guidelines

Antiplatelet therapy, mainly consisting of aspirin and P2Y12 receptor antagonists, is the cornerstone of the pharmacological treatment and prevention of atherothrombotic diseases. Its use, especially in secondary cardiovascular prevention, has significantly improved patient clinical outcomes in the last decades. Primary safety endpoint (i.e., bleeding complications) remain a major drawback of antiplatelet drugs. National and international societies have published and regularly updated guidelines for antiplatelet therapy aiming to provide clinicians with practical recommendations for a better handling of these drugs in various clinical settings. Many recommendations find common ground between international guidelines, but certain strategies vary across the countries, particularly with regard to the choice of molecules, dosage, and treatment duration. In this review, we detail and discuss the main antiplatelet therapy indications in the light of the different published guidelines and the significant number of recently published clinical trials and meta-analyses and highlight the areas that deserve further investigation in order to improve antiplatelet therapy in patients with atherothrombotic diseases

Facilitators and barriers experienced by federal cross-sector partners during the implementation of a healthy eating campaign

To identify facilitators and barriers that Health Canada’s (HC) cross-sector partners experienced while implementing the Eat Well Campaign: Food Skills (EWC; 2013–2014) and describe how these experiences might differ according to distinct partner types. A qualitative study using hour-long semi-structured telephone interviews conducted with HC partners that were transcribed verbatim. Facilitators and barriers were identified inductively and analysed according partner types. Implementation of a national mass-media health education campaign. Twenty-one of HC’s cross-sector partners (food retailers, media and health organizations) engaged in the EWC. Facilitators and barriers were grouped into seven major themes: operational elements, intervention factors, resources, collaborator traits, developer traits, partnership factors and target population factors. Four of these themes had dual roles as both facilitators and barriers (intervention factors, resources, collaborator traits and developer traits). Sub-themes identified as both facilitators and barriers illustrate the extent to which a facilitator can easily become a barrier. Partnership factors were unique facilitators, while operational and target population factors were unique barriers. Time was a barrier that was common to almost all partners regardless of partnership type. There appeared to be a greater degree of uniformity among facilitators, whereas barriers were more diverse and unique to the realities of specific types of partner. Collaborative planning will help public health organizations anticipate barriers unique to the realities of specific types of organizations. It will also prevent facilitators from becoming barriers. Advanced planning will help organizations manage time constraints and integrate activities, facilitating implementation

Full-time employment, diet quality and food skills of Canadian parents

Purpose : To explore the associations between full-time employment status, food skills, and diet quality of Canadian parents. Methods : A sample of Canadian parents (n = 767) were invited to complete a web-based survey that included sociodemographic variables, questions about food skills, and a validated food frequency questionnaire. Results were analyzed with linear and logistic regression models, controlling for sociodemographic variables and multiple testing. Results : After controlling for covariates and multiple testing, there were no statistically significant differences in foods skills between parents’ employment status. Time was the most reported barrier for meal preparation, regardless of work status, but was significantly greater for full-time compared with other employment status (P < 0.0001). Additionally, parents who worked full-time had lower odds of reporting food preferences or dietary restrictions (P = 0.0001) and health issues or allergies (P = 0.0003) as barriers to food preparation, compared with parents with other employment status. These results remained statistically significant even after controlling for covariates and multiple testing. Conclusions : Overall, food skills did not differ significantly between parents’ employment status. Time, however, was an important barrier for most parents, especially those working full time. To promote home-based food preparation among parents, strategies to manage time scarcity are needed.Objectif : Explorer les associations entre une situation d’emploi à temps plein, les compétences alimentaires et la qualité de l’alimentation des parents canadiens. Méthodes : Un échantillon de parents canadiens (n = 767) a été invité à répondre à un sondage Web comprenant des variables sociodémographiques, des questions sur les compétences alimentaires et un questionnaire de fréquence alimentaire validé. Les résultats ont été analysés au moyen de modèles de régression linéaire et logistique en tenant compte des variables sociodémographiques et des tests multiples. Résultats : Après avoir tenu compte des covariables et des tests multiples, aucune différence statistiquement significative n’a été observée quant aux compétences alimentaires des parents en fonction de leur situation d’emploi. Le temps était l’obstacle à la préparation des repas le plus souvent mentionné, quelle que soit la situation d’emploi, mais il était considérablement plus important chez les personnes travaillant à temps plein comparativement aux personnes ayant une autre situation d’emploi (P < 0,0001). De plus, les parents qui travaillaient à temps plein avaient moins de chances d’indiquer que leurs préférences ou restrictions alimentaires (P = 0,0001) et leurs problèmes de santé ou leurs allergies (P = 0,0003) étaient des obstacles à la préparation des aliments, comparativement aux parents se trouvant dans une autre situation d’emploi. Ces résultats sont demeurés statistiquement significatifs même après avoir tenu compte des covariables et des tests multiples. Conclusions : Dans l’ensemble, les compétences alimentaires des parents ne différaient pas de façon significative en fonction de leur situation d’emploi. Le temps était cependant un obstacle important pour la plupart des parents, surtout pour ceux qui travaillent à temps plein. Afin de favoriser la préparation des aliments à la maison par les parents, des stratégies pour les aider à gérer le manque de temps sont nécessaires

The potential of collaborative learning as a tool for forensic students: Application to signature examination

Transferring theoretical knowledge to practical skills remains a big challenge in forensic science, especially in questioned documents. The examination of handwriting and signatures requires years of practice to develop the necessary skills. While students (and to some extent the general population) often have the impression that it is easy to differentiate handwriting from different persons, in practice, particularly when dealing with simulated signatures, there is a high risk of reaching a wrong conclusion when questioned document experts do not use a systematic approach and/or are not sufficiently experienced (see for example the famous French Dreyfus case). Thus, a novel teaching approach, based on collaborative learning, has been introduced in a theoretical handwriting class to improve the students’ theoretical knowledge, and additionally make them aware of the limitations of their practical skills and give them tools to improve them in their future practice. Through five activities, the students took the roles of victims, forgers, teachers and experts and created their own learning materials (i.e. signatures and mock casework). During those interactive activities, they learned to describe their signature’s characteristics, intra-variability and complexity, and thus evaluate their own signature’s vulnerability (as potential victims). They learned techniques to simulate signatures and detect the resulting forgeries’ characteristics (in the role of forgers). In the role of teachers, they prepared mock casework scenarios and gave feedback to their colleague’s examination of the produced material. As experts, they carried out signature examination as they would in a proficiency test and were exposed to the difficulties an actual expert may encounter in practice. The evaluation of this novel teaching scenario was very positive, as students learned more extensively the possibilities and limitations of signature comparison. They were more active and motivated in their learning experiences. The teaching team also had an improved experience. Some students complained of an increased workload and imprecise instructions. Improvements were tested and are discussed in this paper

Protecting peatlands requires understanding stakeholder perceptions and relational values: A case study of peatlands in the Yorkshire Dales

Sustainable peatland management is a global environmental governance challenge given peat’s carbon storage. Peatlands worldwide are sites of contested demands between stakeholders with distinct management priorities. In the United Kingdom, peatland management is a focus of political interest for nature-based solutions (NBS), causing tensions with land managers who feel their traditional knowledge is undervalued. Using Q-method (a semi-quantitative method for clarifying distinct viewpoints) with estate managers, gamekeepers, farmers, and employees of land-owning organisations, we explored perceptions around changing upland management in the Yorkshire Dales. Land managers hold strong values of ownership, aesthetics, and stewardship. The prospect of changing management causes fears of losing these relational values alongside instrumental values. Yorkshire Dales stakeholders agreed on NBS aims (reducing flooding, limiting wildfires, protecting wild birds), but disagreed on methods to achieve these. Our research supports engaging local stakeholders at all stages of peatland protection schemes to minimise resentment towards top-down management