Use of remote sensing to quantify forest response following a wildfire in northeast Minnesota

Wildfire is a natural disturbance common to many forested ecosystems of North America. In the fall of 2011, a lightning-ignited wildfire, the Pagami Creek Fire (PCF), burned over 38,000 hectares of forest in northeast Minnesota, most of which occurred in the protected Boundary Waters Canoe Area Wilderness (BWCAW). Satellite remote sensing data has been used within the upper Midwest to detect and quantify forest vegetation extent, composition, and associated structure. However, very few studies have quantified the composition and structure of early seral forest regeneration. We were interested in two objectives: 1) Use a combination of remote sensing imagery (multi-temporal Landsat data and National Agriculture Imagery Program (NAIP) color-infrared aerial imagery) and derived variables to develop models estimating quaking aspen (Populus tremuloides Michx.) regeneration abundance and structure as well as vegetation species richness derived from field plots. These empirical models were used to spatially extrapolate field data to produce continuous spatially explicit biophysical estimates for the entire PCF burned area. 2) Examine hypothesized relationships between pre-fire aspen basal area (BA) and resulting fire severity, and to evaluate whether burn severity and pre-burn aspen BA may explain observed variability in aspen regeneration (size, abundance, and distribution) and vegetation species richness within the PCF burned area. Remote sensing-based models for vegetation species richness (RMSE=2.47 species, Adj. R2=0.60) was the strongest observed, followed by average aspen stem diameter (RMSE=2.21 mm, Adj. R2=0.53). We also generated statistically significant (p \u3c 0.01) empirical models for average aspen basal area, height, density, and percent cover. We used simple linear regression to examine empirical relationships between field measurements of regenerating aspen abundance and vegetation species richness measurements as functions of satellite derived fire severity and pre-fire aspen BA estimates. Aside from one of our aspen measurements (percent foliar cover), there was a significant relationship (p \u3c 0.01) and meaningful coefficient of determination with pre-fire aspen BA and post-fire aspen measurements (Adj. R2 range = 0.33 - 0.12). There was not a significant relationship (p = 0.46) between pre-fire BA and vegetation species richness (Adj. R2 = -0.01). There was not a significant relationship (p-value range = 0.14 - 0.57) between fire severity and all other vegetation recovery response variables. We expect both forest managers and ecologists to benefit from the ability to detect, model, and quantify early-seral stage forest regeneration over large, often remote, areas. Such remote sensing methods may further facilitate understanding of landscape scale processes (pattern, distribution, and abundance) with respect to fire and other stand-replacing forest disturbances in this and similar forested environments

Redesigning the Senior Leader Engagement Program of the United States Africa Command

AFRICOM conducts hundreds of senior leader engagements (SLEs) each year throughout the African continent in order to create strategic partnerships and military relationships that preserve American interests abroad. While AFRICOM has been planning and executing these engagements since the inception of the organization in 2008, it lacks a well-defined method to systemize its SLE process. As a result, SLE development is largely ad hoc, potentially decreasing the strategic effectiveness of the engagements and increasing their cost. This paper delineates a decision-making framework to redesign and enhance AFRICOM’s SLE program. In particular, it posits a multiple objective decision analysis model that quantifies key stakeholder values and develops several alternatives for future evaluation. Of note, potential solutions imagine a more expansive system where subsets of Senior Leaders (SLs) are assigned to clusters of African countries based on the SLs’ similarity to countries within each cluster, providing a basis for relationship ownership and mutual trust

Modeling Habitat Associations for the Common Loon (\u3cem\u3eGavia immer\u3c/em\u3e) at Multiple Scales in Northeastern North America

Common Loon (Gavia immer) is considered an emblematic and ecologically important example of aquatic-dependent wildlife in North America. The northern breeding range of Common Loon has contracted over the last century as a result of habitat degradation from human disturbance and lakeshore development. We focused on the state of New Hampshire, USA, where a long-term monitoring program conducted by the Loon Preservation Committee has been collecting biological data on Common Loon since 1976. The Common Loon population in New Hampshire is distributed throughout the state across a wide range of lake-specific habitats, water quality conditions, and levels of human disturbance. We used a multiscale approach to evaluate the association of Common Loon and breeding habitat within three natural physiographic ecoregions of New Hampshire. These multiple scales reflect Common Loon-specific extents such as territories, home ranges, and lake-landscape influences. We developed ecoregional multiscale models and compared them to single-scale models to evaluate model performance in distinguishing Common Loon breeding habitat. Based on information-theoretic criteria, there is empirical support for both multiscale and single-scale models across all three ecoregions, warranting a model-averaging approach. Our results suggest that the Common Loon responds to both ecological and anthropogenic factors at multiple scales when selecting breeding sites. These multiscale models can be used to identify and prioritize the conservation of preferred nesting habitat for Common Loon populations

DNA-binding by Haemophilus influenzae and Escherichia coli YbaB, members of a widely-distributed bacterial protein family

BACKGROUND: Genes orthologous to the ybaB loci of Escherichia coli and Haemophilus influenzae are widely distributed among eubacteria. Several years ago, the three-dimensional structures of the YbaB orthologs of both E. coli and H. influenzae were determined, revealing a novel tweezer -like structure. However, a function for YbaB had remained elusive, with an early study of the H. influenzae ortholog failing to detect DNA-binding activity. Our group recently determined that the Borrelia burgdorferi YbaB ortholog, EbfC, is a DNA-binding protein. To reconcile those results, we assessed the abilities of both the H. influenzae and E. coli YbaB proteins to bind DNA to which B. burgdorferi EbfC can bind. RESULTS: Both the H. influenzae and the E. coli YbaB proteins bound to tested DNAs. DNA-binding was not well competed with poly-dI-dC, indicating some sequence preferences for those two proteins. Analyses of binding characteristics determined that both YbaB orthologs bind as homodimers. Different DNA sequence preferences were observed between H. influenzae YbaB, E. coli YbaB and B. burgdorferi EbfC, consistent with amino acid differences in the putative DNA-binding domains of these proteins. CONCLUSION: Three distinct members of the YbaB/EbfC bacterial protein family have now been demonstrated to bind DNA. Members of this protein family are encoded by a broad range of bacteria, including many pathogenic species, and results of our studies suggest that all such proteins have DNA-binding activities. The functions of YbaB/EbfC family members in each bacterial species are as-yet unknown, but given the ubiquity of these DNA-binding proteins among Eubacteria, further investigations are warranted

Central Asia and the globalisation of the contemporary legal consciousness

What is the logic which governs the processes of legal globalization? How does the transnational proliferation of legal forms operate in the contemporary geo-juridical space? What are the main defining characteristics of the currently dominant mode of transnational legal consciousness and how can the concept of legal consciousness help us understand better the historical ebb and flow of the Western-led projects of good governance promotion in regions like Central Asia after the fall of the Soviet Union? Using Duncan Kennedy’s seminal essay Three Globalizations of Law and Legal Thought as its starting platform, this essay seeks to explore these and a series of other related questions, while also drawing on the work of the Greek Marxist lawyer-philosopher Nicos Poulantzas to help elucidate some latent analytical stress-points in Kennedy’s broader theoretical framework. Reacting against the neo-Orientalist tone adopted across much of the contemporary field of Central Asian studies, it develops an alternative account of the internal history of the legal-globalizational encounter between the Western-based reform entrepreneurs and the national legal-political elites in Central Asia in the post-1991 period, complementing it with a detailed description of the general institutional and discursive structures within which this encounter took place

Escherichia coli O157:H7 in Feral Swine near Spinach Fields and Cattle, Central California Coast1

We investigated involvement of feral swine in contamination of agricultural fields and surface waterways with Escherichia coli O157:H7 after a nationwide outbreak traced to bagged spinach from California. Isolates from feral swine, cattle, surface water, sediment, and soil at 1 ranch were matched to the outbreak strain

BpaB, a Novel Protein Encoded by the Lyme Disease Spirochete\u27s Cp32 Prophages, Binds to Erp Operator 2 DNA

Borrelia burgdorferi produces Erp outer surface proteins throughout mammalian infection, but represses their synthesis during colonization of vector ticks. A DNA region 5′ of the start of erp transcription, Operator 2, was previously shown to be essential for regulation of expression. We now report identification and characterization of a novel erp Operator 2-binding protein, which we named BpaB. erp operons are located on episomal cp32 prophages, and a single bacterium may contain as many as 10 different cp32s. Each cp32 family member encodes a unique BpaB protein, yet the three tested cp32-encoded BpaB alleles all bound to the same DNA sequence. A 20-bp region of erp Operator 2 was determined to be essential for BpaB binding, and initial protein binding to that site was required for binding of additional BpaB molecules. A 36-residue region near the BpaB carboxy terminus was found to be essential for high-affinity DNA-binding. BpaB competed for binding to erp Operator 2 with a second B. burgdorferi DNA-binding protein, EbfC. Thus, cellular levels of free BpaB and EbfC could potentially control erp transcription levels

\u3cem\u3eBorrelia burgdorferi\u3c/em\u3e EbfC Defines a Newly-Identified, Widespread Family of Bacterial DNA-Binding Proteins

The Lyme disease spirochete, Borrelia burgdorferi, encodes a novel type of DNA-binding protein named EbfC. Orthologs of EbfC are encoded by a wide range of bacterial species, so characterization of the borrelial protein has implications that span the eubacterial kingdom. The present work defines the DNA sequence required for high-affinity binding by EbfC to be the 4 bp broken palindrome GTnAC, where ‘n’ can be any nucleotide. Two high-affinity EbfC-binding sites are located immediately 5′ of B. burgdorferi erp transcriptional promoters, and binding of EbfC was found to alter the conformation of erp promoter DNA. Consensus EbfC-binding sites are abundantly distributed throughout the B. burgdorferi genome, occurring approximately once every 1 kb. These and other features of EbfC suggest that this small protein and its orthologs may represent a distinctive type of bacterial nucleoid-associated protein. EbfC was shown to bind DNA as a homodimer, and site-directed mutagenesis studies indicated that EbfC and its orthologs appear to bind DNA via a novel α-helical ‘tweezer’-like structure