Role of intestinal microbiota in regulation of immune reactions of gut-associated lymphoid tissue under stress and following the modulation of its composition by antibiotics and probiotics administration

Over the past two decades, active study of the microbial ecosystem of the host organism gastrointestinal tract has led to the recognition of gut microbiome as a "key player" that carries a significant immune pressure and is responsible both for the course of physiological processes and for the development of pathological conditions in humans and animals. A vast number of bacteria living in the human gastrointestinal tract are considered as an organ functioning in dialogue in formation of immunological tolerance, the regulation of normal functional activity of the immune system and maintaining the intestinal mucosa homeostasis. However, disturbances in interaction between these physiological systems is closely related to the pathogenesis of different immune-mediated diseases. In turn, in a large number of works chronic social stress, along with the use of antibiotics, pre- and probiotics, is recognized as one of the leading factors modulating in the microbiota of the gastrointestinal tract. This review focuses on the role of the gut microbiome in the regulation of immune responses of GALT under stress and modulation of its composition by antibiotics and probiotics administration

Anomia is present pre-symptomatically in frontotemporal dementia due to MAPT mutations

© The Author(s) 2022. Springer Nature Switzerland AG. Part of Springer Nature. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Introduction: A third of frontotemporal dementia (FTD) is caused by an autosomal-dominant genetic mutation in one of three genes: microtubule-associated protein tau (MAPT), chromosome 9 open reading frame 72 (C9orf72) and progranulin (GRN). Prior studies of prodromal FTD have identified impaired executive function and social cognition early in the disease but few have studied naming in detail. Methods: We investigated performance on the Boston Naming Test (BNT) in the GENetic Frontotemporal dementia Initiative cohort of 499 mutation carriers and 248 mutation-negative controls divided across three genetic groups: C9orf72, MAPT and GRN. Mutation carriers were further divided into 3 groups according to their global CDR plus NACC FTLD score: 0 (asymptomatic), 0.5 (prodromal) and 1 + (fully symptomatic). Groups were compared using a bootstrapped linear regression model, adjusting for age, sex, language and education. Finally, we identified neural correlates of anomia within carriers of each genetic group using a voxel-based morphometry analysis. Results: All symptomatic groups performed worse on the BNT than controls with the MAPT symptomatic group scoring the worst. Furthermore, MAPT asymptomatic and prodromal groups performed significantly worse than controls. Correlates of anomia in MAPT mutation carriers included bilateral anterior temporal lobe regions and the anterior insula. Similar bilateral anterior temporal lobe involvement was seen in C9orf72 mutation carriers as well as more widespread left frontal atrophy. In GRN mutation carriers, neural correlates were limited to the left hemisphere, and involved frontal, temporal, insula and striatal regions. Conclusion: This study suggests the development of early anomia in MAPT mutation carriers, likely to be associated with impaired semantic knowledge. Clinical trials focused on the prodromal period within individuals with MAPT mutations should use language tasks, such as the BNT for patient stratification and as outcome measures.he Dementia Research Centre is supported by Alzheimer's Research UK, Alzheimer's Society, Brain Research UK, and The Wolfson Foundation. This work was supported by the NIHR UCL/H Biomedical Research Centre, the Leonard Wolfson Experimental Neurology Centre (LWENC) Clinical Research Facility, and the UK Dementia Research Institute, which receives its funding from UK DRI Ltd, funded by the UK Medical Research Council, Alzheimer's Society and Alzheimer's Research UK. JDR is supported by the Miriam Marks Brain Research UK Senior Fellowship and has received funding from an MRC Clinician Scientist Fellowship (MR/M008525/1) and the NIHR Rare Disease Translational Research Collaboration (BRC149/NS/MH). This work was also supported by the MRC UK GENFI grant (MR/M023664/1), the Bluefield Project and the JPND GENFI-PROX grant (2019-02248). This research was supported by the NIHR Cambridge Biomedical Research Centre (BRC-1215-20014). The views expressed are those of the authors and not necessarily those of the NIHR or the Department of Health and Social Care. MB is supported by a Fellowship award from the Alzheimer’s Society, UK (AS-JF-19a-004-517). MB’s work is also supported by the UK Dementia Research Institute which receives its funding from DRI Ltd, funded by the UK Medical Research Council, Alzheimer’s Society and Alzheimer’s Research UK. RC/CG are supported by a Frontotemporal Dementia Research Studentships in Memory of David Blechner funded through The National Brain Appeal (RCN 290173). Several authors of this publication are members of the European Reference Network for Rare Neurological Diseases—Project ID No 739510. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the framework of the Munich Cluster for Systems Neurology (EXC 2145 SyNergy—ID 390857198).info:eu-repo/semantics/publishedVersio

Nutrient status and plankton of nearshore water area of Kronotsky Gulf in August 2015

Firstly we carried out a hydrological and hydrobiological study of the nearshore area, at a depth up to 15 m of Kronotsky Gulf (Kronotsky Reserve, Kamchatka). We have shown that the washout from rivers influences significantly the hydrochemical status of nearshore areas. This parameter determines the level of organic and mineral forms in the compound of phosphorus and nitrogen pools and the content of iron in sea water. The hydrochemical status (particularly – the content of organic compounds) influences the structure of phytoplankton. Diatoms dominate in the locations with relatively high concentrations of mineral forms of phosphorus and nitrogen. In locations with an increased level of these elements in the contents of organic compounds, heterotrophic dinophytes and rotifers dominate. Lists of species of phyto- and zooplankton and their quantitative assessment are presented

REGULATION OF NUMBER OF SULFATE-REDUCING BACTERIA IN ACTIVATED SLUDGE UNDER THE OZONATION IN LOW DOSES

Abstract. Aim. Excessive growth of sulfate reducing bacteria in aeration tanks indicates the prevalence of anaerobic conditions in activated sludge, as a result of which the processes of aerobic oxidation of organic matter of wastewater deteriorate and the quality of treated water in general decreases. Until recently, ozone was used in water treatment as the active oxidizer, only to disinfect sewage. However, the hot interest is ozonation in low doses, which does not lead to total extinction in microbiological cenosis, but only corrects the number of bacteria and microbiological processes in sludge. Methods. In this research were applied classical microbiological cultural methods and enumeration of sulfate-reducing bacteria under most probable number method. Dehydrogenase activity ware measured by Gunter technique. Results. This paper discusses about impact of ozonation in low doses to quantity of sulfure reducing bacteria in activated sludge. Describes possible corrective actions aimed at regulating the processes of sulfate reduction in activated sludge. Conclusions. The effect of ozonation in low doses on sulfate-reducing bacteria in active sludge has been established. The increase of dehydrogenase activity with the use of ozonation has been demonstrated

Photobiomodulation for Correction of Systemic Disorders of Experimental Pain Syndromes

The development of anti-pain technologies in the complex treatment of pain syndromes is one of the most urgent tasks of modern medicine. We undertook a placebo-controlled experimental study of the therapeutic potential of low-intensity laser radiation when applied to acupuncture points that are directly related to the autonomic nervous system. The adaptation effect of puncture photobiomodulation on the induction of stress-mediated autonomic reactions, oxidative metabolism and microcirculation in animals during the acute phase of pain stress was revealed. The data obtained are of interest for use in the complex rehabilitation of patients with pain syndromes

Fluorescent RET-Based Chemosensor Bearing 1,8-Naphthalimide and Styrylpyridine Chromophores for Ratiometric Detection of Hg²⁺ and Its Bio-Application

Dyad compound NI-SP bearing 1,8-naphthalimide (NI) and styrylpyridine (SP) photoactive units, in which the N-phenylazadithia-15-crown-5 ether receptor is linked with the energy donor naphthalimide chromophore, has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In an aqueous solution, NI-SP selectively responds to the presence of Hg2+ via the enhancement in the emission intensity of NI due to the inhibition of the photoinduced electron transfer from the receptor to the NI fragment. At the same time, the long wavelength fluorescence band of SP, arising as a result of resonance energy transfer from the excited NI unit, appears to be virtually unchanged upon Hg2+ binding. This allows self-calibration of the optical response. The observed spectral behavior is consistent with the formation of the (NI-SP)·Hg2+ complex (dissociation constant 0.13 ± 0.04 µM). Bio-imaging studies showed that the ratio of fluorescence intensity in the 440–510 nm spectral region to that in the 590–650 nm region increases from 1.1 to 2.8 when cells are exposed to an increasing concentration of mercury (II) ions, thus enabling the detection of intracellular Hg2+ ions and their quantitative analysis in the 0.04–1.65 μM concentration range

Tracing the Food Web of Changing Arctic Ocean: Trophic Status of Highly Abundant Fish, <i>Gasterosteus aculeatus</i> (L.), in the White Sea Recovered Using Stomach Content and Stable Isotope Analyses

Studies of dietary preferences of migratory species are of great importance as these species connect food webs of habitats across the migration route and thus represent trophic relationships between the spatially disjointed communities. Here we described the dietary preferences of threespine stickleback G. aculeatus in the White Sea during the spawning season using stable isotope and stomach content analyses. The two analyses suggested that during the spawning season, when sticklebacks spend the majority of their time inshore, their diet consists mostly of benthic species, while at the beginning of the spawning season when fish migrating from the offshore were feeding on plankton. Additionally, we demonstrated that stickleback eggs contributed greatly to the diet of both male and female fish. Using Bayesian mixing modeling, we showed that dietary preferences in females were broader than in males, and more variable during the spawning season. While guarding their nests, males fed almost exclusively on eggs. Both stomach contents and isotope signatures demonstrate that by the end of the spawning season sticklebacks again increase the consumption of plankton. Isotope analysis proved to be a more reliable tool to trace this change than stomach content analysis. Our results show that stable isotope and stomach content analyses are complementary in understanding seasonal changes in the dietary composition of stickleback

Theoretical QTAIM, ELI-D, and Hirshfeld Surface Analysis of the Cu–(H)B Interaction in [Cu₂(<i>bipy</i>)₂B₁₀H₁₀]

Interaction of [Cu₂B₁₀H₁₀] with 2,2′-bipyridine (<i>bipy</i>) afforded a novel binuclear discrete complex of the [Cu₂(<i>bipy</i>)₂B₁₀H₁₀] composition. Two copper­(I) atoms coordinate a bridge boron cage through an apical edge and a triangular BBB face situated at its opposite apical vertices to form four 3c2e (CuHB) and one 2c2e Cu–B bonds. The charge density model was obtained by density functional theory calculations of isolated molecule and crystal. The resultant densities were analyzed using the quantum theory of atoms in molecules (QTAIM) and electron localizability indicator (ELI-D). The geometry and the topological parameters of copper­(I) coordination environment were found to be sensitive to crystal-field effect. An annulus of flat electron density ρ­(<i>r</i>) and small ∇²ρ­(<i>r</i>) is formed at dianion faces. As a result, some of the expected B–B, Cu–B, or Cu–H bond critical points are absent. The topological instability in the region of multicentered bonds is observed. The Cu–B bonding was found to be presumably electrostatic in nature, which could be the reason of topological isomerism for copper­(I) decaborates. The results show that an unambiguous real-space criterion for multicentered bonding between transition metals and polyhedral boron anions is not yet given. The molecular graph for this class of compounds does not provide a definitive picture of the chemical boding and can be complemented with other descriptors, such as virial graphs and the ELI-D distribution