Thyroid-stimulating hormone, leptin and insulin resistance in patients with obesity after bariatric surgery

BACKGROUND: The function of the thyroid gland effects on obesity and comorbidities. It has been proven for bariatric surgery to be the most effective in obesity treatment. AIM: to evaluate the dynamics of body weight, thyroid status, leptin and insulin resistance in obese patients after bariatric surgery. MATERIALS AND METHODS: 74 obese patients were observed after bariatric surgery (sleeve gastrectomy – 42, gastric bypass - 32); initial body mass index (BMI), thyroid stimulating hormone, free T4, fasting plasma leptin, insulin and glucose were estimated; the insulin resistance index HOMA-IR was calculated. The dynamics of body weight was estimated by BMI and the excess BMI loss (% EBMIL). After 3 years of follow-up, 48 patients were examined. RESULTS: Subclinical hypothyroidism (SH) was detected in 36.5% of patients with high degrees of obesity. A correlation was found between BMI and TSH level (R=0.5; p=0.01). HOMA-IR was increased in most patients with obesity of the II and III degree (4.8±1.9 ng / ml). In the SH group, the leptin level was significantly higher than in the group with a normal TSH level of 43.0±7.3 ng / ml and 33.2±4.6 ng / ml (p=0.004). Among patients with initial SH, spontaneous reduction of TSH levels occurred in 45% patients 3 years after surgery. CONCLUSIONS: Postoperatively, the BMI decrease was associated with the decrease of TSH, leptin and HOMA-IR. The data obtained may reflect the effect of adipose tissue on the functional state of the thyroid gland in patients with high degrees of obesity after bariatric surgery. This seems to be extremely important for maintaining body weight

Iodine status of Saint Petersburg adult residents

Backgraund. Globally, it is estimated that 2 billion individuals have an insufficient iodine intake. Aim. To assess iodine status and the effectiveness of iodine deficiency prophylaxis in adult population of St. Petersburg. Methods. А retrospective analysis of the prevalence neonatal hyperthyrotropinemia in St. Petersburg for the period 20132014 was made. In a cross-sectional study were included 542 residents of St. Petersburg aged 18-77 years. All participants were divided into 3 groups according to age. Pregnant women were evaluated separately. All participants were questioned, the values of urinary iodine concentration (UIC) were analyzed. Results. The frequency of neonatal thyroid-stimulating hormone concentrations above 5 mU/L was 6.9%. Iodized salt was used by 41.1% of all surveyed persons and 52.2% of pregnant women. Iodine supplements were taken by half of pregnant women. Median UIС of all participants was 91.2 mcg/L and corresponded to mild iodine deficiency. Median UIC in pregnant women was 112.4 mcg/L and corresponded to insufficient iodine intake. Conclusions. The population of St. Petersburg lives in a region of mild iodine deficiency. Prophylaxis of iodine deficiency held in St. Petersburg in 20132015 was not effective enough. Persons of reproductive age and pregnant women had mild iodine deficiency

Ca2+-stimulated Basal Adenylyl Cyclase Activity Localization in Membrane Lipid Microdomains of Cardiac Sinoatrial Nodal Pacemaker Cells*S⃞

Spontaneous, rhythmic subsarcolemmal local Ca2+ releases driven by cAMP-mediated, protein kinase A (PKA)-dependent phosphorylation are crucial for normal pacemaker function of sinoatrial nodal cells (SANC). Because local Ca2+ releases occur beneath the cell surface membrane, near to where adenylyl cyclases (ACs) reside, we hypothesized that the dual Ca2+ and cAMP/PKA regulatory components of automaticity are coupled via Ca2+ activation of AC activity within membrane microdomains. Here we show by quantitative reverse transcriptase PCR that SANC express Ca2+-activated AC isoforms 1 and 8, in addition to AC type 2, 5, and 6 transcripts. Immunolabeling of cell fractions, isolated by sucrose gradient ultracentrifugation, confirmed that ACs localize to membrane lipid microdomains. AC activity within these lipid microdomains is activated by Ca2+ over the entire physiological Ca2+ range. In intact SANC, the high basal AC activity produces a high level of cAMP that is further elevated by phosphodiesterase inhibition. cAMP and cAMP-mediated PKA-dependent activation of ion channels and Ca2+ cycling proteins drive sarcoplasmic reticulum Ca2+ releases, which, in turn, activate ACs. This feed forward “fail safe” system, kept in check by a high basal phosphodiesterase activity, is central to the generation of normal rhythmic, spontaneous action potentials by pacemaker cells

Reflexive constructions in the world's languages

This landmark publication brings together 28 papers on reflexive constructions in languages from all continents, representing very diverse language types. While reflexive constructions have been discussed in the past from a variety of angles, this is the first edited volume of its kind. All the chapters are based on original data, and they are broadly comparable through a common terminological framework. The volume opens with two introductory chapters by the editors that set the stage and lay out the main comparative concepts, and it concludes with a chapter presenting generalizations on the basis of the studies of individual languages

Reflexive constructions in the world's languages

This landmark publication brings together 28 papers on reflexive constructions in languages from all continents, representing very diverse language types. While reflexive constructions have been discussed in the past from a variety of angles, this is the first edited volume of its kind. All the chapters are based on original data, and they are broadly comparable through a common terminological framework. The volume opens with two introductory chapters by the editors that set the stage and lay out the main comparative concepts, and it concludes with a chapter presenting generalizations on the basis of the studies of individual languages

Reflexive constructions in the world's languages

This landmark publication brings together 28 papers on reflexive constructions in languages from all continents, representing very diverse language types. While reflexive constructions have been discussed in the past from a variety of angles, this is the first edited volume of its kind. All the chapters are based on original data, and they are broadly comparable through a common terminological framework. The volume opens with two introductory chapters by the editors that set the stage and lay out the main comparative concepts, and it concludes with a chapter presenting generalizations on the basis of the studies of individual languages

Reflexive constructions in the world's languages

This landmark publication brings together 28 papers on reflexive constructions in languages from all continents, representing very diverse language types. While reflexive constructions have been discussed in the past from a variety of angles, this is the first edited volume of its kind. All the chapters are based on original data, and they are broadly comparable through a common terminological framework. The volume opens with two introductory chapters by the editors that set the stage and lay out the main comparative concepts, and it concludes with a chapter presenting generalizations on the basis of the studies of individual languages