3 research outputs found
Functional Dynamics of PDZ Binding Domains: A Normal Mode Analysis
PDZ (Post-synaptic density-95/discs large/zonula occludens-1) domains are
relatively small (80 to 120 residues) protein binding modules central in the
organization of receptor clusters and in the association of cellular proteins.
Their main function is to bind C-terminals of selected proteins that are
recognized through specific amino-acids in their carboxyl end. Binding is
associated with a deformation of the PDZ native structure and is responsible
for dynamical changes in regions not in direct contact with the target. We
investigate how this deformation is related to the harmonic dynamics of the PDZ
structure and show that one low-frequency collective normal mode, characterized
by the concerted movements of different secondary structures, is involved in
the binding process. Our results suggest that even minimal structural changes
are responsible of communication between distant regions of the protein, in
agreement with recent Nuclear Magnetic Resonance (NMR) experiments. Thus PDZ
domains are a very clear example of how collective normal modes are able to
characterize the relation between function and dynamics of proteins, and to
provide indications on the precursors of binding/unbonding events.Comment: 25 pages, 10 figures, submitted to Biophysical Journa