Transformation of Resource Allocation Processes Based on Digital Technologies

The article seeks to reveal the features of the transformation of the processes of resource provision of enterprises. In fact, a mechanism for providing resources based on digital technologies for the implementation of enterprise development programs has been developed. New approaches to the interaction of enterprises for creating digital models of development process management are formulated. A system of interaction between enterprises in the digital economy is proposed, which makes it possible to make decisions when allocating resources effectively. Among the main problems identified is the low efficiency of the methodological mechanism for resource allocation. Based on the outcomes, it can be concluded that the creation of digital technology is becoming one of the main advantages of the mutual linking of sources of the resource base at all levels of management

BRCA1 4153delA founder mutation in Russian ovarian cancer patients

The BRCA1 4153delA allele is frequently referred to as the Russian founder mutation, as it was initially detected in several cancer families from Moscow. Our earlier studies have demonstrated 1% occurrence of BRCA1 4153delA heterozygosity in familial and/or early-onset and/or bilateral Russian breast cancer (BC) patients. Since literature data suggest that the 4153delA variant is more associated with ovarian cancer (OC) than with BC, we expected to reveal a highly elevated frequency of this genotype in Russian ovarian cancer series. However, real-time allele-specific PCR genotyping has detected only two BRCA1 4153delA carriers out of 177 unselected OC patients (1.1%). Both these carriers were early-onset and had serous carcinomas of grade 3. Thus, our study supports neither the Russian origin of BRCA1 4153delA mutation, nor its selectivity towards ovarian versus breast cancer predisposition

Detection of Apoptosis in Cancer Cells Using Heat Shock Protein 70 and p53 Antibody Conjugated Quantum Dot Nanoparticles

Clinical experience indicates that enhanced level of heat shock protein 70 (Hsp70) and p53 correlates with poor prognosis due to malignant cell overexpression of these proteins in tumor progression. Cadmium selenide quantum dots (QDs) were synthesized in aqueous solution using mercaptopropionic acid and L-cysteine (L-Cys) as ligands. They were conjugated with a monoclonal antibody (Ab) to p53 and cmHp70.1 to Hsp70 for detection of cancer cell apoptosis that was demonstrated in the experiment by fluorescent confocal microscopy both for breast carcinoma cells and for thyroid tissue. It is shown that in comparison with organic dyes, quantum dots have superior photostability of tracking apoptosis in cancer cells for longer time

Knockdown of SF-1 and RNF31 Affects Components of Steroidogenesis, TGFβ, and Wnt/β-catenin Signaling in Adrenocortical Carcinoma Cells

The orphan nuclear receptor Steroidogenic Factor-1 (SF-1, NR5A1) is a critical regulator of development and homeostasis of the adrenal cortex and gonads. We recently showed that a complex containing E3 ubiquitin ligase RNF31 and the known SF-1 corepressor DAX-1 (NR0B1) interacts with SF-1 on target promoters and represses transcription of steroidogenic acute regulatory protein (StAR) and aromatase (CYP19) genes. To further evaluate the role of SF-1 in the adrenal cortex and the involvement of RNF31 in SF-1-dependent pathways, we performed genome-wide gene-expression analysis of adrenocortical NCI-H295R cells where SF-1 or RNF31 had been knocked down using RNA interference. We find RNF31 to be deeply connected to cholesterol metabolism and steroid hormone synthesis, strengthening its role as an SF-1 coregulator. We also find intriguing evidence of negative crosstalk between SF-1 and both transforming growth factor (TGF) β and Wnt/β-catenin signaling. This crosstalk could be of importance for adrenogonadal development, maintenance of adrenocortical progenitor cells and the development of adrenocortical carcinoma. Finally, the SF-1 gene profile can be used to distinguish malignant from benign adrenocortical tumors, a finding that implicates SF-1 in the development of malignant adrenocortical carcinoma

High frequency of BRCA1, but not CHEK2 or NBS1 (NBN), founder mutations in Russian ovarian cancer patients

<p>Abstract</p> <p>Background</p> <p>A significant portion of ovarian cancer (OC) cases is caused by germ-line mutations in BRCA1 or BRCA2 genes. BRCA testing is cheap in populations with founder effect and therefore recommended for all patients with OC diagnosis. Recurrent mutations constitute the vast majority of BRCA defects in Russia, however their impact in OC morbidity has not been yet systematically studied. Furthermore, Russian population is characterized by a relatively high frequency of CHEK2 and NBS1 (NBN) heterozygotes, but it remains unclear whether these two genes contribute to the OC risk.</p> <p>Methods</p> <p>The study included 354 OC patients from 2 distinct, geographically remote regions (290 from North-Western Russia (St.-Petersburg) and 64 from the south of the country (Krasnodar)). DNA samples were tested by allele-specific PCR for the presence of 8 founder mutations (BRCA1 5382insC, BRCA1 4153delA, BRCA1 185delAG, BRCA1 300T>G, BRCA2 6174delT, CHEK2 1100delC, CHEK2 IVS2+1G>A, NBS1 657del5). In addition, literature data on the occurrence of BRCA1, BRCA2, CHEK2 and NBS1 mutations in non-selected ovarian cancer patients were reviewed.</p> <p>Results</p> <p>BRCA1 5382insC allele was detected in 28/290 (9.7%) OC cases from the North-West and 11/64 (17.2%) OC patients from the South of Russia. In addition, 4 BRCA1 185delAG, 2 BRCA1 4153delA, 1 BRCA2 6174delT, 2 CHEK2 1100delC and 1 NBS1 657del5 mutation were detected. 1 patient from Krasnodar was heterozygous for both BRCA1 5382insC and NBS1 657del5 variants.</p> <p>Conclusion</p> <p>Founder BRCA1 mutations, especially BRCA1 5382insC variant, are responsible for substantial share of OC morbidity in Russia, therefore DNA testing has to be considered for every OC patient of Russian origin. Taken together with literature data, this study does not support the contribution of CHEK2 in OC risk, while the role of NBS1 heterozygosity may require further clarification.</p

Analysis of selected parts of the logistics chain in the company Nesmas-M LLC

V teoretické části práce se zaměřím s pomocí odborné literatury na informace, které mi poslouží jako podklady pro praktickou část. V praktické části představím firmu Nesmas-M .s r.o.. Dále popíši vybrané části z logistického řetězce, jedná se o software, systém objednávek, způsob skladování a dopravu. V další části práce pokusím se navrhnout řešení existujicích problémů.The theoretical part will focus with the help of literature on the information that I use as the basis for the practical part. In practical part i will introduce company Nesmas-M LLC and describe chosen components of the logistics chain, it is the software, ordering system, method of storage and transport. In the next section I will try to propose solutions to existing problems

Frequency-Dependent Seismic Reflectivity of Randomly Fractured Fluid-Saturated Media

Fractures exist on a wide range of scales from microns to hundreds of meters. Throughout this scale range, fractures have a significant influence on fluid flow and physical properties of rocks. The average elastic properties of a randomly fractured fluid-filled rock were discussed for different fracture distribution laws in association with the extremely slow and dispersive guided wave propagation within individual fractures. Krauklis wave was used as an asymptotic solution of the fluid interface wave (FIW) equations. Different fracture distribution laws (exponential, power, fractal and gamma laws) within the rock in the seismic range of frequencies ($10-100Hz$) initiated high-velocity dispersion and attenuation of the P-wave. Calculations showed that increase of one order of fracture density enhances velocity dispersion and attenuation by 20\%, in particular, at low seismic frequencies. Different cases of acoustic impedance distributions versus depth for assessing reflection properties from fractured and non-fractured layers have been considered. Results demonstrated the remarkable difference between the P-wave reflection coefficient from the fractured layer and the P-wave reflection coefficient from the non-fractured layer: about 30-40\% decrease in amplitude for the fractured high-impedance layer, about 30-50\% increase of amplitude for the fractured low-impedance layer and about 20\% decrease for the intermediate case. The biggest difference in the behavior of reflection coefficient versus incident angle is observed at seismic low-frequencies ($<15$Hz). The thickness related tuning effect has the different impact on the seismic signal in the fractured and homogeneous layers for all acoustic impedance cases. The approach and results of calculations allow an interpretation of abnormal velocity dispersion, high attenuation, and special behavior of reflection coefficients vs. frequency and angle of incidence as the indicators of fractures. The analysis of the seismic monitoring data from the Royal Center Field, Indiana, indicates the frequency-dependent difference of attenuation and velocity of the P-wave in water saturated and gas saturated formation. It is in agreement with numerical modeling results involving Krauklis wave theory. The difference is bigger at low frequencies. The numerical modeling explains a low-frequency seismic anomaly, detected in fractured zones within source rock in the East-Surgut Basin, Western Siberia. The study of the laboratory measurements on the fractured 3-D printed samples indicates the possibility of a P-wave velocity prediction in the fluid-filled fractured sample based on the velocity of non-fractured porous saturated background.Earth and Atmospheric Sciences, Department o

The green agenda influence on the methodology of housing and communal services pricing

The paper proves the necessity of revising the methodology of housing and communal services pricing in connection with the implementation of the city development concept within the framework of the green agenda and the strategies and programs development aimed at ecologically important problems resolution, clean technologies development, natural resource conservation, and the increase in the population living standards as well. It is shown that the creation of ecologically sustainable urban systems requires not only the development and implementation of innovative technologies and attraction of financial resources but also the assessment of their consequences, such as changes in the volumes of consumed housing and communal services and tariffs for them. Adjustments to the methodology of housing and communal services pricing should ensure the process of providing quality services at affordable prices for consumers. The main results of the study demonstrate that modern cities should utilize innovative solutions aimed at changing the approach to operating and managing housing and communal services to meet the requirements of ecological safety and sustainability. Implementing the recommendations to improve the ecological situation and sustainability of housing and communal services can become a key factor in creating a peaceful, successful, and efficient urban environment

Management Methods by Development of Objects of Energy Supply Taking into Account the Combined Risks

In the article the methods of choice of optimal administrative decisions are examined on the development of objects energy supplies of housing and communal services. That would take into account nascent risks related to investment-building activity of energy supplying enterprises. It is shown that the basic condition of housing and utilities on an energy supply is the building of objects with a subsequent production, distribution and realization of electric energy, that allows at their reproduction to provide quality and reliability of energy supply to the consumers. Also the questions of decline of risks and exposure of factors, influencing on the processes of management development of objects of energy supply in the field of housing and utilities, estimation of organizationally-economic reliability and combined risks, estimation of possibility of the use of innovative energy technologies, alternative sources of energy supply, are investigated in the article. It is fixed, that risk indexes must be appraised not only on the investment stage of building object, but also on his operating stage (after commissioning) on the basis of account of the great number of risks characterizing the combined risk

Management Methods by Development of Objects of Energy Supply Taking into Account the Combined Risks

In the article the methods of choice of optimal administrative decisions are examined on the development of objects energy supplies of housing and communal services. That would take into account nascent risks related to investment-building activity of energy supplying enterprises. It is shown that the basic condition of housing and utilities on an energy supply is the building of objects with a subsequent production, distribution and realization of electric energy, that allows at their reproduction to provide quality and reliability of energy supply to the consumers. Also the questions of decline of risks and exposure of factors, influencing on the processes of management development of objects of energy supply in the field of housing and utilities, estimation of organizationally-economic reliability and combined risks, estimation of possibility of the use of innovative energy technologies, alternative sources of energy supply, are investigated in the article. It is fixed, that risk indexes must be appraised not only on the investment stage of building object, but also on his operating stage (after commissioning) on the basis of account of the great number of risks characterizing the combined risk