Confronting the opioid crisis: Practical pain management and strategies: AOA 2018 critical issues symposium

The United States is in the midst of an opioid crisis. Clinicians have been part of the problem because of overprescribing of narcotics for perioperative pain management. Clinicians need to understand the pathophysiology and science of addiction to improve perioperative management of pain for their patients. Multiple modalities for pain management exist that decrease the use of narcotics. Physical strategies, cognitive strategies, and multimodal medication can all provide improved pain relief and decrease the use of narcotics. National medical societies are developing clinical practice guidelines for pain management that incorporate multimodal strategies and multimodal medication. Changes to policy that improve provider education, access to naloxone, and treatment for addiction can decrease narcotic misuse and the risk of addiction

A NEW WAY OF IDENTIFICATION OF YEASTS: USE OF NIR TECHNIQUE

Four strains of Saccharomyces genera were investigated: S. pastorianus CBS 1503, S. cerevisiae CBS 1395, S. cerevisiae CB 67 and S. cerevisiae CB 89. The aim of our work was to test the suitability of NIR technique for rapid identification of different yeasts. Yeasts were grown in batch culture and samples were taken from the exponential and stationary growth stages, later ones were divided into parts and one of them was heat-shocked. The four yeasts tested were morphologically different, the CBS signed showed oval, the CB signed ones had round shaped cells. Optimum growth temperatures were all in the range of 30-35 ⁰C, and at 30⁰C growth rates of mixed cultures did not differ significantly from those of monocultures. The SDS-PAGE protein prints of the four strains were different both in the number of subfractions apd their molecular mass distributions. This method is suitable to characterize and differentiate the investigated yeasts . Quantitative evaluation ofthe SDS-PAGE patterns enables us to detect infections in the pure culture . NIR spectra of the investigated strains were significantly different. The growth stage or heat-shock treatment had serious effect on the spectrum. Mixtures of two pure cultures (0, 10, 20 ... 90% 'infection') showed NIR spectra in between those of the pure culture and even 10% contamination caused a significant difference

Species Richness and Diversity Reveal that Human-Modified Environments are not Wastelands

Urbanization is often associated with the loss of habitats for many species but the pattern of invertebrate abundance in increasingly human-dominated urban areas is less well documented particularly for the expanding urban Africa. This study investigated the composition and community structure of spiders in relation to human activities at the foot of the Uluguru Mountains in Morogoro city, Tanzania. Three different sampling methods: pitfall trapping, sweep netting and active searching were used to collect spiders in the study area and to allow comparison between structurally different habitat sites. Spider abundance differed significantlybetween habitat types and was influenced by the sampling methods used. Family diversity and richness showed no significant differences across the sites. The spider species consisted of primarily three functional groups: ground wanderers, web builders and plant wanderers, and showed no within-group differences in abundance between sites. Similarity index between the study sites revealed a considerable overlap in the spider families present. No correlation between habitat variables: tree shade, herbaceous cover and ground cover and spider functional groups were found, suggesting that habitat alteration has minimal effect on the abundance of these invertebrate taxa. Further, cluster analysis at the family level revealed that spiders formed clusters on the basis of their hunting strategies, suggesting the avoidance of competition among spider guilds. This study provides insight into the importance of human-dominated areas on invertebrate biodiversity and serves as a basis for future work

Effects of Inflow Condition on RANS and LES Predictions of the Flow around a High-Rise Building

An increasing number of engineering applications require accurate predictions of the flow around buildings to guarantee performance and safety. This paper investigates the effects of variations in the turbulent inflow, as predicted in different numerical simulations, on the flow pattern prediction around buildings, compared to wind tunnel tests. Turbulence characteristics were assessed at several locations around a model square high-rise building, namely, above the roof region, at the pedestrian level, and in the wake. Both Reynolds-averaged Navier–Stokes (RANS, where turbulence is fully modelled) equations and large-eddy simulation (LES, where turbulence is partially resolved) were used to model an experimental setup providing validation for the roof region. The performances of both techniques were compared in ability to predict the flow features. It was found that RANS provides reliable results in regions of the flow heavily influenced by the building model, and it is unreliable where the flow is influenced by ambient conditions. In contrast, LES is generally reliable, provided that a suitable turbulent inflow is included in the simulation. RANS also benefits when a turbulent inflow is provided in simulations. In general, LES should be the methodology of choice if engineering applications are involved with the highly separated and turbulent flow features around the building, and RANS provides reliable information when regions of high wind speed and low turbulence are investigated

'Follow the innovation' - a joint experimentation and learning approach to transdisciplinary innovation research

The ZEF-UNESCO project on Sustainable Management of Land and Water Resources in Uzbekistan addresses the environmental, social and economic problems in the Khorezm province, located within the so-called Aral Sea Zone in Uzbekistan. Currently in its seventh year (third phase), the project aims “to provide sustainable solutions to the Aral Sea region through a holistic approach, combining technology, policy and institutional options developed in cooperation with local and international stakeholders” as stated on the project website. Consequently, the mutually enriching interplay of multiple disciplines is a precondition for a successful project. Inter- and eventually transdisciplinary research, rather than the mere co-existence of different disciplines captured under the notion of ‘multidisciplinarity’ is the chosen and actively facilitated approach. A work package is specifically designed to foster the creation of inter- and transdisciplinary research teams around innovation packages developed by the project and identified as possessing ‘plausible promises’ to take hold in the Khorezm agricultural system. To trigger and sustain this process, a stepwise approach is taken. In a workshop series, four ‘Follow the Innovation (FTI)’ teams of scientists surrounding innovations were formed, roadmaps prepared and a set of tools and skills for stakeholder interaction taught. A participatory joint experimentation and learning approach was chosen to validate innovations in the farmer’s setting. Between the workshops, all four FTI teams are supported and accompanied by a full-time FTI facilitator in their stakeholder outreach and designing and implementing processes of joint experimentation and learning with stakeholders. After the first steps of stakeholder involvement are made and contacts well established, the stakeholders, as partners take part in all following training workshops. Here the further processes of testing, experimenting and jointly adapting the developed innovation packages to match the local needs as well as bio-physical and socio-economic environments are jointly designed with the partners. Additionally this process is continuously monitored, documented, critically discussed and adjusted accordingly by all partners. This paper seeks to illustrate and discuss the experiences collected so far in nurturing a transdisciplinary process of joint experimentation and learning between researchers and local stakeholders, each in their behaviours, attitudes and actions shaped by the context and culture of their places of origin. These include amongst others the academic disciplines of the researchers, the research project, the local Uzbek setting, shaped by strong hierarchical structures and a post-soviet system of knowledge governance. Thus, diverse forms and contents of knowledge, carried by uniquely socialised actors, shall be mutually exchanged and lead to the joint development of new knowledge, to innovations that ‘make sense’ in the rural setting of Khorezm/Uzbekistan. The process design, its strengths and weaknesses are outlined and recommendations for improvement discussed

Tracking residual organochlorine pesticides (OCPs) in green, herbal, and black tea leaves and infusions of commercially available tea products marketed in Poland

The content of residual organochlorine pesticides (OCPs) was examined in green, herbal, and black tea leaves as well as in their infusions prepared from tea products marketed in the main supermarkets in Poland. It was found that the detected mean levels of organochlorine residues in tea leaves ranged from <LOD to 16.36 ng g−1 dry weight. Among hexachlorocyclohexane isomers, γ-HCH in green tea occurred in the highest concentrations. Among dichlorodiphenyltrichloroethane (DDT) metabolites the highest level of p,p′DDT (1.96 ng g−1 dw) was in green tea samples. The transfer of OCPs from tea leaves to brew was investigated. The present study revealed that during the infusion process, a significant percentage of the residues, particularly pesticides with high water solubility, were transferred to the infusions. The obtained results show that the percentage transfer of each pesticides from tea to the tea infusions ranged from 6.74% (heptachlor) to 86.6% (endrin). The detected residues were below current MRLs for these pesticides. © 2018 Taylor & Francis Group, LLC

Enhancing transgenic pea (Pisum sativum L.) resistance against fungal diseases through stacking of two antifungal genes (chitinase and glucanase).

One way of enhancing and broadening resistance of plants to different biotic and abiotic stresses is to combine transgenes expressing several genes into a single line. This can be done using different strategies such as crossing, single vector with multiple genes, co-transformation, sequential transformation and IRES elements. In the present study conventional crossing method was used. Parental transgenic lines transformed via Agrobacterium tumefasciens-mediated gene transformation with pGreenII binary vector harbouring a bar gene as selectable marker in combination with the family 19 chitinase gene from Streptomyces olivaceoviridis for one line and 1,3-β-glucanase from barley (Hordeum vulgare) for the other line were used for crossing. Both chitinase and glucanase genes were cloned into pGreenII vector under the control of the constitutive double 35S-promoter from cauliflower mosaic virus. Progenies expressing the two genes were characterised at the molecular level using PCR, RT-PCR and Southern blot analysis, as well as segregation and stability studies of the respective expression levels. Leaf paint assay was used as functional test for herbicide resistant gene. Stable inheritance of the antifungal genes in the transgenic plants was demonstrated. The synergistic effect of crossed plants was tested using in vitro assay which shows higher inhibition of spore germination

Oxidized xylan additive for nanocellulose films – A swelling modifier

Polymeric wood hemicelluloses are depicted to join cellulose, starch and chitosan as key polysaccharides for sustainable materials engineering. However, the approaches to incorporate hemicelluloses in emerging bio-based products are challenged by lack of specific benefit, other than the biomass-origin, although their utilization would contribute to sustainable material use since they currently are a side stream that is not valorized. Here we demonstrate wood-xylans as swelling modifiers for neutral and charged nanocellulose films that have already entered the sustainable packaging applications, however, suffer from humidity sensitivity. The oxidative modification is used to modulate the water-solubility of xylan and hence enable adsorption in an aqueous environment. A high molecular weight grade, hence less water-soluble, adsorbed preferentially on the neutral surface while the adsorbed amount on a negatively charged surface was independent of the molecular weight, and hence, solubility. The adsorption of the oxidized xylans on a neutral cellulose surface resulted in an increase in the amount of water in the film while on the negatively charged cellulose the total amount of water decreased. The finding of synergy of two hygroscopic materials to decrease swelling in hydrophilic bio-polymer films demonstrates the oxidized macromolecule xylan as structurally functional component in emerging cellulose products