Systemic Treatment of Pediatric Psoriasis: A Review

<p><b>Article full text</b></p> <p><br></p> <p>The full text of this article can be found here<b>. </b><a href="https://link.springer.com/article/10.1007/s13555-016-0117-6">https://link.springer.com/article/10.1007/s13555-016-0117-6</a></p><p></p> <p><br></p> <p><b>Provide enhanced content for this article</b></p> <p><br></p> <p>If you are an author of this publication and would like to provide additional enhanced content for your article then please contact <a href="http://www.medengine.com/Redeem/”mailto:[email protected]”"><b>[email protected]</b></a>.</p> <p><br></p> <p>The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.</p> <p><br></p> <p>Other enhanced features include, but are not limited to:</p> <p><br></p> <p>• Slide decks</p> <p>• Videos and animations</p> <p>• Audio abstracts</p> <p>• Audio slides</p

Systemic Treatment of Adult Atopic Dermatitis: A Review

<p><b>Article full text</b></p> <p><br></p> <p>The full text of this article can be found here<b>. </b><a href="https://link.springer.com/article/10.1007/s13555-016-0170-1">https://link.springer.com/article/10.1007/s13555-016-0170-1</a></p><p></p> <p><br></p> <p><b>Provide enhanced content for this article</b></p> <p><br></p> <p>If you are an author of this publication and would like to provide additional enhanced content for your article then please contact <a href="http://www.medengine.com/Redeem/”mailto:[email protected]”"><b>[email protected]</b></a>.</p> <p><br></p> <p>The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.</p> <p><br></p> <p>Other enhanced features include, but are not limited to:</p> <p><br></p> <p>• Slide decks</p> <p>• Videos and animations</p> <p>• Audio abstracts</p> <p>• Audio slides</p

Phenolics from <i>Castanea sativa</i> leaves and their effects on UVB-induced damage

<p>The phytochemical investigation of the methanol extract of the leaves of <i>Castanea sativa</i> Mill.<i>,</i> source of the Italian PGI (Protected Geographical Indication) product ‘Marrone di Roccadaspide’ (Campania region) afforded as main compounds crenatin (<b>1</b>), chestanin (<b>2</b>), gallic acid (<b>3</b>), cretanin (<b>4</b>), 5-<i>O</i>-<i>p</i>-coumaroylquinic acid (<b>5</b>), <i>p</i>-methylgallic acid (<b>6</b>) and quercetin-3-<i>O</i>-glucoside (<b>7</b>). To quantify the isolated compounds a LC-ESI(QqQ)MS method working with a very sensitive and selective mass tandem experiment called Multiple Reaction Monitoring (MRM) has been developed. Moreover the antioxidant capacity by TEAC assay and the ability of compounds <b>1</b>–<b>7</b> to protect HaCaT human keratinocytes from UVB-induced damage has been investigated.</p

Glycopeptides in the P2 repertoire of Hpt tryptic digest from plasma of patients in disease remission.

a<p>The asparagine residue harbouring the glycan is underlined.</p>b<p>Quantitative analysis for relative abundance of species was obtained taking into account all the observed multicharged ions. The values are expressed as means (from three technical replicates) ± standard deviations. Glycan species are indicated with the same nomenclature previously used for pHpt-P and pHpt-N <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052040#pone.0052040-Maresca1" target="_blank">[16]</a>.</p

Mass spectrum of the P2 glycopeptide repertoire from skin of patients.

<p>Purified sHpt-P was digested by trypsin, and the resulting fragments were fractionated by UPLC and analyzed by ESI-MS. Positive ions of P2 (NLFL<u>N</u> HSE<u>N</u>ATAK) glycopeptides from Hpt of skin of patients is shown. Glycopeptide peaks are indicated with their observed mass value (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052040#pone-0052040-t003" target="_blank">Table 3</a>). The glycoforms found only in sHpt-P and not in pHpt-N and in pHpt-P are indicated. Peaks with mass attributable to non-glycosylated species were ignored.</p

Normal phase HPLC pattern of 2AB-glycans from pHpt-N, pHpt-P and sHpt-P.

<p>Purified pHpt-N, pHpt-P, and sHpt-P were deglycosylated by treatment with PNGase F, and their glycans were labelled by 2 aminobenzamide. After solid phase extraction, the glycans were fractionated by HPLC using a TSK-gel Amide-80 column (4.6×250 mm) with a linear gradient of ammonium formate at pH 4.4 (87.5 to 162.5 mM) with CH₃CN (65 to 35%) in 75 min, at 0.4 ml/min flow rate. Elution was monitored by measuring the label fluorescence at 425 nm (λ_EX = 360 nm). Panel A: glycans from pHpt-N. Panel B: glycans from pHpt-P. Panel C: glycans from sHpt-P. The GU ladder represents the migration of labelled oligosacharides with different units of glucose. The marked peaks were used for comparative analysis.</p

Glycopeptides in the P3 repertoire of Hpt tryptic digest from patients in disease remission.

a<p>The asparagine residue harbouring the glycan is underlined.</p>b<p>Quantitative analysis for relative abundance of species was obtained taking into account all the observed multicharged ions. The values are expressed as means (from three technical replicates) ± standard deviations.</p><p>Glycan species are indicated with the same nomenclature previously used for pHpt-P and pHpt-N <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052040#pone.0052040-Maresca1" target="_blank">[16]</a>.</p

Glycopeptides in the P2 repertoire of Hpt tryptic digest from skin of patients and controls, and their relative abundance.

a<p>The asparagine residue harbouring the glycan is underlined.</p>b<p>Quantitative analysis for relative abundance of species was obtained taking into account all the observed multicharged ions. The values are expressed as means (from three technical replicates) ± standard deviations.</p>c<p>ND = not detected. The ratio signal-to-noise = 3 was used as detection threshold.</p><p>Glycan species are indicated with the same nomenclature previously used for pHpt-P and pHpt-N <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052040#pone.0052040-Maresca1" target="_blank">[16]</a>.</p

Glycopeptides in the P1 repertoire of Hpt tryptic digest from skin of patients and controls, and their relative abundance.

a<p>The asparagine residue harbouring the glycan is underlined.</p>b<p>Quantitative analysis for relative abundance of species was obtained taking into account all the observed multicharged ions. The values are expressed as means (from three technical replicates) ± standard deviations.</p>c<p>ND = not detected. The ratio signal-to-noise = 3 was used as detection threshold.</p><p>Glycan species are indicated with the same nomenclature previously used for pHpt-P and pHpt-N <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052040#pone.0052040-Maresca1" target="_blank">[16]</a>.</p

Mass spectrum of the P3 glycopeptide repertoire from skin of patients.

<p>Purified sHpt-P was digested by trypsin, and the resulting fragments were fractionated by UPLC and analyzed by ESI-MS. Positive ions of P3 (VVLHP<u>N</u>YSQVDIGLIK) glycopeptides from Hpt of skin of patients is shown. Glycopeptide peaks are indicated with their observed mass value (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052040#pone-0052040-t004" target="_blank">Table 4</a>) and chain structure. Peaks with mass attributable to non-glycosylated species were ignored.</p