Novel bioresorbable tricalcium phosphate/polyhydroxyoctanoate (TCP/PHO) composites as scaffolds for bone tissue engineering applications

Development of new composite materials for bone tissue engineering is a constantly growing field of medicine. Therefore there is a continuous need in creating novel materials that can not only regenerate the defected tissue but also nourish it while the healing process progresses. Here we present a novel type of composite material that fulfils these requirements. The study describes creation of a composite with macroporous bioceramic core that is infiltrated with a thin biopolymer layer. The ceramic component, namely tricalcium phosphate (TCP), due to its mechanistic and bioactive properties may promote new bone creation as shown through the in vitro studies. To the best of our knowledge the coating layer was created for the first time from a representative of bacterially derived medium chain length polyhydroxyalkanoate polymers (mcl-PHA), namely polyhydroxyoctanoate (PHO). This polymer layer not only profoundly changed the stress-strain characteristics of the bioceramic foam but also released (R)-3-hydroxyacids and their dimers/trimers to the investigated environment. In the manuscript we have in depth characterised these materials employing a set of basic procedures, through 3D structure reconstruction and finishing with prolonged in vitro experiments

Sposób wytwarzania wysokokreatywnych proszków fosforanów wapnia opis patentowy nr 190486 /

Zgłoszono 9 marca 1999 r.Zgłoszenie ogłoszono 11 września 2000 BUP 19/00.O udzieleniu patentu ogłoszono 30 grudnia 2005 WUP 12/05.Nr zgłosz. P 331907.Tyt. z ekranu tyt.Dostępny także w wersji drukowanej.Tryb dostępu: Internet

Sposób otrzymywania ceramicznego tworzywa implantacyjnego opis patentowy nr 154957 /

Zgłoszono 18 kwietnia 1988 r.Zgłoszenie ogłoszono 30 października 1989 r.Opublikowano 30 czerwca 1992 r.Nr zgłosz. P 271942.Tyt. z ekranu tyt.Dostępny także w wersji drukowanej.Tryb dostępu: Internet

Sposób wytwarzania bioceramicznego nośnika leków opis patentowy nr 190485 /

Zgłoszono 9 marca 1999 r.Zgłoszenie ogłoszono 11 września 2000 BUP 19/00.O udzieleniu patentu ogłoszono 30 grudnia 2005 WUP 12/05.Nr zgłosz. P 331906.Tyt. z ekranu tyt.Dostępny także w wersji drukowanej.Tryb dostępu: Internet

Sposób wytwarzania wysokoporowatego tworzywa ceramicznego opis patentowy nr 154958 /

Zgłoszono 22 kwietnia 1988 r.Zgłoszenie ogłoszono 30 października 1989 r.Opublikowano 30 kwietnia 1992 r.Nr zgłosz. P 272058.Tyt. z ekranu tyt.Dostępny także w wersji drukowanej.Tryb dostępu: Internet

Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose

Recently, the attention has been drawn to complex systems – biomicroconcretes composed of a bone cement matrix and resorbable granules or microspheres. This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties. The aim of this work was to study α-TCP-GCSD-polymer interactions and to compare the impact of organic additives on the physicochemical properties of biomicroconcretes. Methods: Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used. Results: The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7–12 MPa) and clinically relevant setting times (8–33 min). Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa). It has also been found that the dissolution-precipitation reactions of the α-TCP were retarded with the addition of chitosan and acetic acid. This effect was not observed in the case of methylcellulose and sodium alginate. Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid. Conclusions: Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo. It has been demonstrated that the presence of CSD granules and polymers influenced the physicochemical properties of composites

Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

Bioactive, chemically bonded bone substitutes with antibacterial properties are highly recommended for medical applications. In this study, biomicroconcretes, composed of silicon modified (Si-αTCP) or non-modified α-tricalcium phosphate (αTCP), as well as hybrid hydroxyapatite/chitosan granules non-modified and modified with gold nanoparticles (AuNPs), were designed. The developed biomicroconcretes were supposed to combine the dual functions of antibacterial activity and bone defect repair. The chemical and phase composition, microstructure, setting times, mechanical strength, and in vitro bioactive potential of the composites were examined. Furthermore, on the basis of the American Association of Textile Chemists and Colorists test (AATCC 100), adapted for chemically bonded materials, the antibacterial activity of the biomicroconcretes against S. epidermidis, E. coli, and S. aureus was evaluated. All biomicroconcretes were surgically handy and revealed good adhesion between the hybrid granules and calcium phosphate-based matrix. Furthermore, they possessed acceptable setting times and mechanical properties. It has been stated that materials containing AuNPs set faster and possess a slightly higher compressive strength (3.4 ± 0.7 MPa). The modification of αTCP with silicon led to a favorable decrease of the final setting time to 10 min. Furthermore, it has been shown that materials modified with AuNPs and silicon possessed an enhanced bioactivity. The antibacterial properties of all of the developed biomicroconcretes against the tested bacterial strains due to the presence of both chitosan and Au were confirmed. The material modified simultaneously with AuNPs and silicon seems to be the most promising candidate for further biological studies