Additional file 2: of Comprehensive metabolic profiling of chronic low-grade inflammation among generally healthy individuals

Supplemental Tables. (XLSX 30 kb

Additional file 1: of Comprehensive metabolic profiling of chronic low-grade inflammation among generally healthy individuals

Supplemental Matrial, Methods and Figures. (DOCX 678 kb

Corrected p-values (false discovery rate; FDR) from linear regression analyses with osteocalcin concentration as exposure and plasma (left upper panel) or urine (right lower panel) metabolites as outcomes.

<p>Models were adjusted for age, sex, waist circumference and physical activity. The upper right panel contains results for metabolites present in both fluids. Within each panel the dotted lines denote the significance threshold of FDR<0.05. Metabolites are colored if they are significantly associated in plasma (blue), urine (orange) or in both (red), respectively. Metabolites marked with a diamond exceed the plotting range. Metabolites highlighted by increased point size and darker colors persisted significant even after further adjustment for hormone intake, estimated glomerular filtration rate and smoking behavior. Corresponding beta estimates and FDR-values can be found in supplemental Tables <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184721#pone.0184721.t001" target="_blank">1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184721#pone.0184721.t002" target="_blank">2</a>. Metabolites numbered with 1 to 7 are named in the lower left panel. Triangles indicate the direction of the association in plasma or urine, with ▲ indicating positive associations.</p

Plasma and urine metabolites significantly associated with the serum osteocalcin concentration.

<p>Plasma and urine metabolites significantly associated with the serum osteocalcin concentration.</p

General characteristics of the study population.

<p>General characteristics of the study population.</p

ANP32B-dependent precipitation of HeV M.

<p>(A) Western Blot detection of ANP32B and HeV M in cell lysates (input) and after purification of Strep-ANP32B (eluate). (B) silver gel analysis of purified protein samples. The identities of abundant signals at 40 and 35 kDa were confirmed by mass spectrometry as HeV M and ANP32B, respectively.</p

Over-expression of ANP32B results in specific nuclear accumulation of HeV M.

<p>(a–c) No nuclear accumulation was detectable in HEK 293T cells in the absence of over-expressed ANP32B. (d–f) At ANP32B over-expression, HeV M accumulated in the nucleus. (g–i) Leptomycin B led to nuclear accumulation of HeV M without over-expression of ANP32B. (j–l) Leptomycin B led to nuclear accumulation of rabies virus P. (m–o) In the absence of Leptomycin B, rabies virus P (RABV P) was not detected in the nucleus. (p–r) ANP32B over-expression did not induce nuclear accumulation of RABV P.</p

ANP32B-dependent nuclear retention of M in Nipah virus infected cells.

<p>After infection of mCherry-ANP32B expressing A549 cells at an MOI of 0.5, nuclear accumulation of NiV M was observed (arrows). In contrast, no accumulation was detectable in nuclei (DAPI-stained; blue) without mCherry-ANP32B (arrowheads). Shown are composites of two images. Scale bar: 10 µM.</p

ANP32B-dependent precipitation of HeV M.

<p>(A) Western Blot detection of ANP32B and HeV M in cell lysates (input) and after purification of Strep-ANP32B (eluate). (B) silver gel analysis of purified protein samples. The identities of abundant signals at 40 and 35 kDa were confirmed by mass spectrometry as HeV M and ANP32B, respectively.</p

Virus release is not inhibited by shRNA knock-down of ANP32B expression.

<p>(A) Western Blots confirmed knock down of ANP32B protein in HEK-293T cells that stably express shRNAs against ANP32B mRNA (shRNA 765 and 767). Control cells shRNA 782 and 784 expressed irrelevant shRNAs and in authentic HEK293T cells ((−) shRNA) no shRNAs were expressed. (B) Growth curves for Nipah virus revealed that Nipah virus production was not affected by the shRNA knock down.</p