The protective effect of Liza klunzingeri protein hydrolysate on carbon tetrachloride-induced oxidative stress and toxicity in male rats

Objective(s): Today, consumers are looking for food products providing health benefits in addition to meeting the basic nutritional needs of the body. This study aimed to evaluate the antioxidant and cytotoxic effects of Liza klunzingeri protein hydrolysate both in vivo and in vitro. Materials and Methods: Fish protein hydrolysate (FPH) was prepared using enzymatic hydrolysis with papain. In vitro antioxidant activity was assessed using five different antioxidant assays. The cytotoxic effect on 4T1 cell line was evaluated using the MTT assay. The distribution of the molecular weight of FPH was measured using HPLC. In the in vivo study, CCl4-exposed Wistar rats were orally treated with FPH (150, 300, and 600 mg/kg) or gallic acid (50 mg/kg) for 28 consecutive days. Results: Enzymatic hydrolysis gave hydrolysate rich in low molecular weight peptides (<1000 Da) with strong free radicals (ABTS, DPPH, and OH) scavenging activity and cytotoxicity. Treatment of CCl4-exposed rats with all doses of FPH significantly lowered serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). FPH at doses of 300 and 600 mg/kg significantly decreased lipid peroxidation and improved total antioxidant capacity in serum, liver, and kidney of the CCl4 exposed rats. All doses of L.klunzingeri protein hydrolysate reduced CCl4-induced nitric oxide production of the kidney. Liver histopathological damage caused by CCl4 also ameliorated with all doses of FPH. Conclusion: L. klunzingeri protein hydrolysate can be considered as a functional food to alleviate oxidative stress. Keywords Author Keywords:Antioxidant activity; Cytotoxicity; Liza klunzingeri; Oxidative stress; Protein hydrolysat

Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos.

Purpose To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos. Methods Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos. Results Irrespective of TSA treatment, POU5F1 expression was not detected until 8–16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly increased POU5F1 expression, and the yield and quality of cloned embryo development compared to control. Conclusion The POU5F1 expression of cloned embryos is strictly controlled by the stage of embryo development and may not be altered by TSA-mediated changes occur in DNA-methylation and histone-acetylation of the genome

Antioxidant properties of Klunzinger’s mullet (Liza klunzingeri) protein hydrolysates prepared with enzymatic hydrolysis using a commercial protease and microbial hydrolysis with Bacillus licheniformis

Antioxidant activity of Klunzinger's mullet (Liza klunzingeri) muscle hydrolysates obtained using Bacillus licheniformis fermentation and enzymatic hydrolysis was determined. Hydrolysates obtained after 6 days of fermentation with B. licheniformis showed the highest free radical scavenging activity, metal chelating ability and ferric reducing antioxidant power (FRAP) (P ≤ 0.05). Microbial fermentation led to a higher percentage of small peptides and higher solubility compared with Alcalase hydrolysis (P ≤ 0.05). Hydrolysates showing the highest antioxidant properties attenuated serum, liver, and kidney oxidative stress biomarkers in male Wister rats stressed by carbon tetrachloride (P ≤ 0.05). At 300 mg/kg oral administration, hydrolysates increased serum, renal, and hepatic total antioxidant capacity (TAC) (P ≤ 0.05) and reduced their elevated levels of malondialdehyde (MDA), nitric oxide (NO•), and serum liver enzymes (AST, ALP, and ALT) (P ≤ 0.05). The hydrolysates were able to ameliorate hepatic damage by reducing necrosis, fatty changes, and inflammation. Results showed the antioxidant and hepato-toxic protective activities of Klunzinger’s mullet muscle hydrolysates obtained using microbial fermentation, which may, therefore, potentially be considered as a functional food ingredien