Sperm specific proteins-potential candidate molecules for fertility control

The increase in population growth rate warrants the development of additional contraceptive methods that are widely acceptable, free from side effects and less expensive. Immunocontraception, and in particular the targeting of antibodies to gamete-specific antigens implicated in sperm egg binding and fertilization, offers an attractive approach to control fertility. The development of a contraceptive vaccine based on sperm antigen represents a promising approach to contraception. In mammals, fertilization is completed by the direct interaction of sperm and egg, a process mediated primarily by sperm surface proteins. Sperm have proteins that are unique, cell specific, immunogenic and accessible to antibodies. A few of the sperm specific proteins have been isolated and characterized. The antibodies raised against the sperm specific antigens have proved to be extremely effective at reducing sperm-egg interaction in vitro; fertility trials in sub-human primates would eventually prove the effectiveness of the sperm antigens in terms of contraceptive efficacy

Effect of Temperature and Branched Crosslinkers on Supported Graphene Oxide Pervaporation Membranes for Ethanol Dehydration

We describe the performance of graphene oxide (GO) membranes stabilized by crosslinkers and supported on polyethersulfone films in the dehydration of ethanol in a continuous cross-flow pervaporation set-up. We used two crosslinker species with branched structures (humic acid-like substances derived from urban waste and a synthetic hyperbranched polyol). The supported crosslinked GO films were prepared by rod coating on a polyethersulfone ultrafiltration membrane. Pervaporation experiments were carried out at temperatures of 40, 50, 60 and 70 °C. When the feed comprised pure water and ethanol, a much higher flux of water than ethanol was observed at all temperatures through GO films stabilized by the two crosslinkers (humic acid, GO-HAL, and the synthetic hyperbranched polyol, GO-HBPO), indicating the separation ability of these crosslinked membranes. For feed mixtures of water and ethanol, the GO-HAL and GO-HBPO membranes showed good separation performances by producing permeates with a significantly higher water content than the feed at all temperatures

Approximating Dominating Set on Intersection Graphs of Rectangles and L-frames

We consider the Minimum Dominating Set (MDS) problem on the intersection graphs of geometric objects. Even for simple and widely-used geometric objects such as rectangles, no sub-logarithmic approximation is known for the problem and (perhaps surprisingly) the problem is NP-hard even when all the rectangles are "anchored" at a diagonal line with slope -1 (Pandit, CCCG 2017). In this paper, we first show that for any epsilon>0, there exists a (2+epsilon)-approximation algorithm for the MDS problem on "diagonal-anchored" rectangles, providing the first O(1)-approximation for the problem on a non-trivial subclass of rectangles. It is not hard to see that the MDS problem on "diagonal-anchored" rectangles is the same as the MDS problem on "diagonal-anchored" L-frames: the union of a vertical and a horizontal line segment that share an endpoint. As such, we also obtain a (2+epsilon)-approximation for the problem with "diagonal-anchored" L-frames. On the other hand, we show that the problem is APX-hard in case the input L-frames intersect the diagonal, or the horizontal segments of the L-frames intersect a vertical line. However, as we show, the problem is linear-time solvable in case the L-frames intersect a vertical as well as a horizontal line. Finally, we consider the MDS problem in the so-called "edge intersection model" and obtain a number of results, answering two questions posed by Mehrabi (WAOA 2017)

Abdominopelvic mass in postmenopausal female: a diagnostic dilemma

Abdominopelvic masses have varied presentations and pose a diagnostic challenge especially in postmenopausal women where a detailed evaluation is needed to rule out malignancy. Here we report a case of postmenopausal woman with diagnostic discrepancies in clinical findings, radiological investigations and histopathological observation

Heat shock protein 70-2 (HSP70-2) overexpression in breast cancer

Supplementary Methods, Supplementary table, Supplementary Figure Legends. (DOCX 41 kb

Effect of vam fungi and applied phosphorus through stcr precision model on growth, yield and nutrient dynamics in maize in acid alfisol

The Inoculation of three VAM cultures viz. local culture (Glomus mosseae), VAM culture from IARI, New Delhi (Glomus mosseae) and a culture from The Energy Research Institute (TERI), New Delhi (Glomus Intraradices) alone or with increasing applied phosphorus levels from 25 to 75% of recommended P2OS based on soil test crop response (STCR) precision model improved the plant height, shoot and root dry matter accumulation, root length and root weight density as well as yield attributes of ralnfed maize in an acid alflsol of NW Himalayas. It was revealed that sole application of any of 3 VAM fungi did not have pronounced effect on phenologlcal stages though combined application of VAM cultures with increasing P levels from 25 to 75% of recommended P2O5 reduced the days to various phenologlcal stages, it was reported that sole application of these 3 VAM cultures improved the maize grain yield by 17.10 to 25.36% over control. Increase in P levels from 25 to 75% of recommended P2O5 besides VAM inoculation resulted in consistent and significant Improvement In grain, stover and biological yield of maize. 75% of recommended P2O5 alongwith VAM cultures achieved the goal of targeted yield (40 q ha"1) of maize, thus, saving the applied P to the tune of about 25% without Impairing the soil fertility in the present stud

Influence of Vesicular Arbuscular Mycorrhizal Fungi and Applied Phosphorus on Root Colonization in Wheat and Plant Nutrient Dynamics in a Phosphorus-Deficient Acid Alfisol of Western Himalayas

Vesicular arbuscular mycorrhizal (VAM) fungi symbiosis confers benefits directly to the host plant's growth and yield through acquisition of phosphorus and other macro- and micronutrients, especially from phosphorus (P)–deficient acidic soils. The inoculation of three VAM cultures [viz., local culture (Glomus mosseae), VAM culture from Indian Agricultural Research Institute (IARI), New Delhi (Glomus mosseae), and a culture from the Centre for Mycorrhizal Research, Energy Research Institute (TERI), New Delhi (Glomus intraradices)] along with P fertilization in wheat in a P-deficient acidic alfisol improved the root colonization by 16–24% while grain and straw yields increased by 12.6–15.7% and 13.4–15.4%, respectively, over the control. Uptake of nitrogen (N), P, potassium (K), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) was also improved with VAM inoculation over control, but the magnitude of uptake was significantly greater only in the cases of P, Fe, Zn, and Cu. Inoculation of wheat with three VAM cultures in combination with increasing inorganic P application from 50% to 75% of the recommended P2O5 dose to wheat through the targeted yield concept following the soil-test crop response (STCR) precision model resulted in consistent and significant improvement in grain and straw yield, macronutrient (NPK) uptake, and micronutrient (Fe, Mn, Zn, Cu) uptake in wheat though root colonization did not improve at P2O5 doses beyond 50% of the recommended dose. The VAM cultures alone or in combination with increasing P levels from 50% to 75% P2O5 dose resulted in reduction of diethylenetriaminepentaacetic acid (DTPA)–extractable micronutrient (Fe, Mn, Zn, Cu) contents in P-deficient acidic soil over the control and initial fertility status, although micronutrient contents were relatively greater in VAM-supplied plots alone or in combination with 50% to 75% P2O5 dose over sole application of 100% P2O5 dose, thereby indicating the positive role of VAM in nutrient mobilization and nutrient dynamics in the soil–plant system. There was significant improvement in available N and P status in soil with VAM inoculation coupled with increasing P levels upto 75% P2O5 dose, although the greatest P buildup was obtained with sole application of 100% P2O5 dose. The TERI VAM culture (Glomus intraradices) showed its superiority over the other two cultures (Glomus mosseae) in terms of crop yield and nutrient uptake in wheat though the differences were nonsignificant among the VAM cultures alone or at each P level. Overall, it was inferred that use of VA-mycorrhizal fungi is beneficial under low soil P or in low input (nutrient)–intensive agroecosystems

Improving Phosphorus Use through Co-inoculation of Vesicular Arbuscular Mycorrhizal Fungi and Phosphate-Solubilizing Bacteria in Maize in an Acidic Alfisol

Performance of three vesicular arbuscular mycorrhizal (VAM) fungi cultures and a phosphate-solubilizing bacteria (PSB) culture alone or in combination with or without 75% of the recommended P2O5 dose based on soil-test crop response model was examined in maize in a phosphorus (P)-deficient acidic Alfisol in a glasshouse pot experiment. Sole application of VAM besides co-inoculation with PSB (Pseudomonas striata) and inorganic P stimulated mycorrhizal root colonization. Sole application of PSB, VAMT (Glomus intraradices), and VAMI (Glomus mosseae) as well as co-inoculation of VAM with PSB significantly improved crop productivity besides grain protein content, thus indicating a synergistic interaction between VAM and PSB. Application of VAMT or VAMI + PSB + 75% P2O5 remained at par with sole application of 100% P2O5 dose with regard to productivity, nutrient uptake, and soil fertility status (particularly P), thus indicating economization of fertilizer P to the tune of about 25% without compromising crop productivity and soil fertility in an acidic Alfisol