Dynamics of Viral Infection and Evolution of SARS-CoV-2 Variants in the Calabria Area of Southern Italy

In this study, we report on the results of SARS-CoV-2 surveillance performed in an area of Southern Italy for 12 months (from March 2021 to February 2022). To this study, we have sequenced RNA from 609 isolates. We have identified circulating VOCs by Sanger sequencing of the S gene and defined their genotypes by whole-genome NGS sequencing of 157 representative isolates. Our results indicated that B.1 and Alpha were the only circulating lineages in Calabria in March 2021; while Alpha remained the most common variant between April 2021 and May 2021 (90 and 73%, respectively), we observed a concomitant decrease in B.1 cases and appearance of Gamma cases (6 and 21%, respectively); C.36.3 and Delta appeared in June 2021 (6 and 3%, respectively); Delta became dominant in July 2021 while Alpha continued to reduce (46 and 48%, respectively). In August 2021, Delta became the only circulating variant until the end of December 2021. As of January 2022, Omicron emerged and took over Delta (72 and 28%, respectively). No patient carrying Beta, Iota, Mu, or Eta variants was identified in this survey. Among the genomes identified in this study, some were distributed all over Europe (B1_S477N, Alpha_L5F, Delta_T95, Delta_G181V, and Delta_A222V), some were distributed in the majority of Italian regions (B1_S477N, B1_Q675H, Delta_T95I and Delta_A222V), and some were present mainly in Calabria (B1_S477N_T29I, B1_S477N_T29I_E484Q, Alpha_A67S, Alpha_A701S, and Alpha_T724I). Prediction analysis of the effects of mutations on the immune response (i.e., binding to class I MHC and/or recognition of T cells) indicated that T29I in B.1 variant; A701S in Alpha variant; and T19R in Delta variant were predicted to impair binding to class I MHC whereas the mutations A67S identified in Alpha; E484K identified in Gamma; and E156G and ΔF157/R158 identified in Delta were predicted to impair recognition by T cells. In conclusion, we report on the results of SARS-CoV-2 surveillance in Regione Calabria in the period between March 2021 and February 2022, identified variants that were enriched mainly in Calabria, and predicted the effects of identified mutations on host immune response

Il Futuro della Cybersecurity in Italia: Ambiti Progettuali Strategici

Il presente volume nasce come continuazione del precedente, con l’obiettivo di delineare un insieme di ambiti progettuali e di azioni che la comunità nazionale della ricerca ritiene essenziali a complemento e a supporto di quelli previsti nel DPCM Gentiloni in materia di sicurezza cibernetica, pubblicato nel febbraio del 2017. La lettura non richiede particolari conoscenze tecniche; il testo è fruibile da chiunque utilizzi strumenti informatici o navighi in rete. Nel volume vengono considerati molteplici aspetti della cybersecurity, che vanno dalla definizione di infrastrutture e centri necessari a organizzare la difesa alle azioni e alle tecnologie da sviluppare per essere protetti al meglio, dall’individuazione delle principali tecnologie da difendere alla proposta di un insieme di azioni orizzontali per la formazione, la sensibilizzazione e la gestione dei rischi. Gli ambiti progettuali e le azioni, che noi speriamo possano svilupparsi nei prossimi anni in Italia, sono poi accompagnate da una serie di raccomandazioni agli organi preposti per affrontare al meglio, e da Paese consapevole, la sfida della trasformazione digitale. Le raccomandazioni non intendono essere esaustive, ma vanno a toccare dei punti che riteniamo essenziali per una corretta implementazione di una politica di sicurezza cibernetica a livello nazionale. Politica che, per sua natura, dovrà necessariamente essere dinamica e in continua evoluzione in base ai cambiamenti tecnologici, normativi, sociali e geopolitici. All’interno del volume, sono riportati dei riquadri con sfondo violetto o grigio; i primi sono usati nel capitolo introduttivo e nelle conclusioni per mettere in evidenza alcuni concetti ritenuti importanti, i secondi sono usati negli altri capitoli per spiegare il significato di alcuni termini tecnici comunemente utilizzati dagli addetti ai lavori. In conclusione, ringraziamo tutti i colleghi che hanno contribuito a questo volume: un gruppo di oltre 120 ricercatori, provenienti da circa 40 tra Enti di Ricerca e Università, unico per numerosità ed eccellenza, che rappresenta il meglio della ricerca in Italia nel settore della cybersecurity. Un grazie speciale va a Gabriella Caramagno e ad Angela Miola che hanno contribuito a tutte le fasi di produzione del libro. Tra i ringraziamenti ci fa piacere aggiungere il supporto ottenuto dai partecipanti al progetto FILIERASICURA

Interaction of Cadmium with Glutathione and Photosynthesis in Developing Leaves and Chloroplasts of Phragmites australis (Cav.) Trin. ex Steudel

We investigated how the presence of cadmium (Cd) at the emergence of Phragmites australis Trin. (Cav.) ex Steudel plants from rhizomes interacted with leaf and chloroplast physiological and biochemical processes. About 8.5 nmol Cd mg(–1) chlorophyll was found in leaves, and 0.83 nmol Cd mg(–1) chlorophyll was found in chloroplasts of plants treated with 50 μm Cd. As a result, a 30% loss of chlorophyll was measured concomitantly with a comparable percentage reduction in light-saturated photosynthesis. Rubisco content and activity were lowered by 10% and 60%, respectively. Antioxidant activity was stimulated by Cd treatment and was associated with an increase in the glutathione and pyridine pools, and with a larger pool of reduced glutathione. It is suggested that the glutathione pool and its predominance in the reduced state protected the activity of many key photosynthetic enzymes against the thiophilic binding of Cd. Chloroplast ultrastructure was not significantly altered with 50 μm treatment and the efficiency of photosystem II, measured as the fluorescence ratio F(v)/F(m), remained high because F(0) and F(m) were proportionally decreased. In plants treated with 100 μm Cd, all effects were exacerbated, but F(v)/F(m) remained close to that of control leaves and the glutathione and pyridine nucleotides pools were lowered. The results suggest that glutathione exerted a direct important protective role on photosynthesis in the presence of Cd

Evaluation of Multiple Responses Associated with Arsenic Tolerance and Accumulation in Pteris vittata L. Plants Exposed to High As Concentrations under Hydroponics

Chinese brake fern (Pteris vittata L.) is recognized as an arsenic hyperaccumulating plant. Mechanisms underlying this capability and the associated hypertolerance have been described even if not completely elucidated. In this study, with the aim to expand the knowledge on the matter, an experimental trial was developed to investigate an array of responses, at the morphological, physiological, and biochemical level, in P. vittata plants exposed to high As concentrations in a long-term experiment under hydroponics. Results confirmed the ability of fern plants to both tolerate and accumulate a remarkable amount of As, especially in fronds. Notably, in As-treated plants, a far higher As content was detected in young fronds compared to old fronds, with bioaccumulation (BCF) and translocation (Tf) factors in accordance. At the biochemical level, As treatment affected macro and micronutrient, thiol, and phytochelatin concentrations in fronds of treated plants differently than that of the control. Physiological measurements accounted for a reduction in the photosynthetic activity of As-treated plants in the absence of visual symptoms of damage. Overall, the observed As tolerance and accumulation processes were discussed, evidencing how young fronds developed during As treatment maintain their physiological status while accumulating a high As content. Such indications could be very useful to improve the effective utilization of this plant species for phytofiltration of As-polluted water

Tolerance to low temperature and paraquat-mediated oxidative stress in two maize genotypes

Tolerance to low temperature and paraquat-mediated oxidative stress was investigated in two Zea mays genotypes, VA36 and A619, grown at 25/22 degrees C and 16/14 degrees C for 50 d after germination. VA36, the tolerant genotype, showed an enhanced resistance to paraquat as compared to A619, the sensitive genotype, when grown at low temperature. In VA36, superoxide dismutase and ascorbate peroxidase activities increased during growth at both 25/22 degrees C or 16/14 degrees C. in A619, superoxide dismutase activity was similar in plants grown at both 16/14 degrees C or 25/22 degrees C. Ascorbate peroxidase activity was always significantly lower in plants grown at low temperature than in plants grown at 25/22 degrees C, The total ascorbate peroxidase activity was correlated with the cytosolic ascorbate peroxidase protein content in all but A619 plants grown at low temperature for 25 d. The isozyme pattern of SOD showed a higher abundance of MnSOD in VA36 than in A619 and of FeSOD in A619 compared to VA36. Growth at low temperature enhanced resistance to paraquat infiltration more in VA36 than in A619. SOD and APX activities were generally higher and more stable with the increase of paraquat concentration in VA36 than in A619. Damage indicated by F-v/F-m and ion leakage after paraquat infiltration were generally higher in plants grown at 25/22 degrees C than at 16/14 degrees C and higher in A619 than in VA36, However, no causal link is proved between the extent of damage and the increase of SOD and APX activities alone. It is suggested that tolerance to oxidative stress requires an integrated enhancement of the antioxidant system

Effect of Rhizobium Sp. Inoculation on N 2 -Fixing and Photosynthetic Activities of Two Cowpea [ Vigna unguiculata (L.) Walp.] Genotypes

Time course of symbiotic N2-fixing and photosynthetic activities during vegetative growth from 30 d after plantation until pod set was measured in the CB5 and 7964 cowpea [Vigna unguiculata (L.) Walp.] genotypes of contrasting senescence traits. At emergence, seedlings were inoculated with a "non-cowpea miscellany" Rhizobium strain generally used to inoculate Cicer arietinum. Maximum N2-fixing activity occurred in inoculated CB5 and 7964 plants about 54 and 68 d after plantation, respectively. A similar temporal shift of maximum was found for net photosynthetic rate (P N), confirming a good coordination between the two processes. A higher P N was found from the first measurements in inoculated plants of both genotypes as compared with uninoculated plants. Apparently, the maximum activity of both N2-fixation and P N was timed to occur at a particular stage of plant ontogeny correlating the high N supply with the high N demand by the plant. Rhizobium inoculation did not significantly affect partitioning coefficients of biomass to various plant organs but extended leaf longevity by about 10 d in the CB5 genotype, retarding thus the monocarpic senescence

Effects of Apirolio Addition and Alfalfa and Compost Treatments on the Natural Microbial Community of a Historically PCB-Contaminated Soil

Polychlorinated biphenyls (PCBs) are ubiquitous and persistent organic pollutants generated exclusively from human sources and found in the environment as several congeners (e.g. Apirolio, produced in Italy and used for electrical transformers). To evaluate the ability of the natural microbial community of historically PCB-contaminated soil to transform or degrade PCBs after fresh contamination through the addition of Apirolio, a microcosm experiment was conducted in a greenhouse for approximately 8 months. Compost and Medicago sativa (alfalfa) were additionally used in the microcosms to stimulate microbial PCB degradation. Chemical analyses were performed to evaluate PCB concentrations in the soil and plant tissue. Changes in the microbial community under the different experimental conditions were evaluated in terms of total abundance, viability, diversity, and activity. Interestingly, the addition of Apirolio did not negatively affect the microbial community but did stimulate the degradation of the freshly added PCBs. The plant and compost co-presence did not substantially increase PCB degradation, but it increased the microbial abundance and activity and the occurrence of α-Proteobacteria and fungi