Adaptive channel selection in IEEE 802.15.4 TSCH networks

Additional files 6: Table S5. Four conjugative transposon gene clusters in the Chryseobacterium indologenes J31 genome

シベリア亜寒帯林における樹冠と林床の植生指数

Clinical characteristic of patients with rheumatoid arthritis (RA) and trauma. (DOC 45 kb

Synthesis and Characterization of Cleavable Core-Cross-Linked Micelles Based on Amphiphilic Block Copolypeptoids as Smart Drug Carriers

Amphiphilic block copolypeptoids consisting of a hydrophilic poly­(<i>N</i>-ethyl glycine) segment and a hydrophobic poly­[(<i>N</i>-propargyl glycine)-<i>r</i>-(<i>N</i>-decyl glycine)] random copolymer segment [PNEG-<i>b</i>-P­(NPgG-<i>r</i>-NDG), EPgD] have been synthesized by sequential primary amine-initiated ring-opening polymerization (ROP) of the corresponding <i>N</i>-alkyl <i>N</i>-carboxyanhydride monomers. The block copolypeptoids form micelles in water and the micellar core can be cross-linked with a disulfide-containing diazide cross-linker by copper-mediated alkyne–azide cycloaddition (CuAAC) in aqueous solution. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis revealed the formation of spherical micelles with uniform size for both the core-cross-linked micelles (CCLMs) and non-cross-linked micelles (NCLMs) precursors for selective block copolypeptoid polymers. The CCLMs exhibited increased dimensional stability relative to the NCLMs in DMF, a nonselective solvent for the core and corona segments. Micellar dissociation of CCLMs can be induced upon addition of a reducing agent (e.g., dithiothreitol) in dilute aqueous solutions, as verified by a combination of fluorescence spectroscopy, size exclusion chromatography (SEC), and ¹H NMR spectroscopic measurement. Doxorubicin (DOX), an anticancer drug, can be loaded into the hydrophobic core of CCLMs with a maximal 23% drug loading capacity (DLC) and 37% drug loading efficiency (DLE). <i>In vitro</i> DOX release from the CCLMs can be triggered by DTT (10 mM), in contrast to significantly reduced DOX release in the absence of DTT, attesting to the reductively responsive characteristic of the CCLMs. While the CCLMs exhibited minimal cytotoxicity toward HepG2 cancer cells, DOX-loaded CCLMs inhibited the proliferation of the HepG2 cancer cells in a concentration and time dependent manner, suggesting the controlled release of DOX from the DOX-loaded CCLMS in the cellular environment

Table5_Genome-wide identification, characterization and expression of HSP 20 gene family in dove.XLSX

Davidia involucrata is a significant living fossil with high abiotic stress tolerance. Although heat shock protein 20 (HSP20) has already been linked to heat stress, nothing is known about HSP20 family protein activities in D. involucrata. The functional dynamics of the D. involucrata HSP20 (DiHSP20) gene family were identified and characterized using a thorough genome-wide investigation. From the genome of D. involucrata, a total of 42 HSP20 genes were identified, which are distributed across 16 chromosomes. The DiHSP20 proteins were grouped into seven separate subfamilies by our phylogenetic analysis, which was validated by the conserved motif composition and gene structure studies. Segmental duplication events were shown to play a crucial role in the expansion of the DiHSP20 gene family. Synteny analysis revealed that 19 DiHSP20 genes of D. involucrata shared a syntenic connection with Arabidopsis genes, 39 with C. acuminata genes, and just 6 with O. sativa genes. Additionally, heat stress differently enhanced the expression levels of D. involucrata HSP20 genes. After 1 hour of heat treatment, the expression levels of most DiHSP20 genes, particularly DiHSP20-7, DiHSP20-29, DiHSP20-30, DiHSP20-32, and DiHSP20-34, were dramatically increased, suggestted that they might be employed as heat tolerance candidate genes. Overall, these findings add to our knowledge of the HSP20 family genes and provide helpful information for breeding heat stress resistance in D. involucrata.</p

Table1_Genome-wide identification, characterization and expression of HSP 20 gene family in dove.XLS

Davidia involucrata is a significant living fossil with high abiotic stress tolerance. Although heat shock protein 20 (HSP20) has already been linked to heat stress, nothing is known about HSP20 family protein activities in D. involucrata. The functional dynamics of the D. involucrata HSP20 (DiHSP20) gene family were identified and characterized using a thorough genome-wide investigation. From the genome of D. involucrata, a total of 42 HSP20 genes were identified, which are distributed across 16 chromosomes. The DiHSP20 proteins were grouped into seven separate subfamilies by our phylogenetic analysis, which was validated by the conserved motif composition and gene structure studies. Segmental duplication events were shown to play a crucial role in the expansion of the DiHSP20 gene family. Synteny analysis revealed that 19 DiHSP20 genes of D. involucrata shared a syntenic connection with Arabidopsis genes, 39 with C. acuminata genes, and just 6 with O. sativa genes. Additionally, heat stress differently enhanced the expression levels of D. involucrata HSP20 genes. After 1 hour of heat treatment, the expression levels of most DiHSP20 genes, particularly DiHSP20-7, DiHSP20-29, DiHSP20-30, DiHSP20-32, and DiHSP20-34, were dramatically increased, suggestted that they might be employed as heat tolerance candidate genes. Overall, these findings add to our knowledge of the HSP20 family genes and provide helpful information for breeding heat stress resistance in D. involucrata.</p

El Diario de Pontevedra : periódico liberal: Ano XXVII Número 7794 - 1910 maio 2

Meta-analysis revealed similar hospital stay in RAMPS and standard procedure. (PNG 9 kb

Asymmetric Total Syntheses of Aspidodasycarpine, Lonicerine, and the Proposed Structure of Lanciferine

Aspidodasycarpine and lonicerine are a pair of epimeric aspidophylline-type alkaloids bearing vicinal quaternary C7 and C16. The first and enantioselective total syntheses of these molecules are described here. A Ru-catalyzed asymmetric transfer hydrogenation established the first stereocenter. An Au-promoted Toste cyclization was exploited to assemble the bridged tetracyclic core and define the geometry of the exocyclic olefin; electron deficient (<i>p</i>-CF₃C₆H₄)₃P was a suitable ligand for this transformation. An aldol condensation followed by an intramolecular indole C3 alkylation constructed the adjacent quaternary C7 and C16 diastereoselectively, leading to a pentacyclic lactol as an advanced common intermediate for synthesizing both alkaloids. The proposed structure of lanciferine, a highly oxidized congener of aspidodasycarpine, was synthesized from the lactol by tuning the oxidation states of various carbons

Total Syntheses of Aflavazole and 14-Hydroxyaflavinine

The first total syntheses of aflavazole (<b>6</b>) and 14-hydroxyaflavinine (<b>8</b>), two sterically congested indole diterpenoids, were accomplished. AlI₃-promoted alkyne Prins cyclization was exploited to construct their key structural motifs. An electrocyclization–aromatization sequence assembled the pentasubstituted arene of <b>6</b>, and a Stille–Migita coupling furnished the tetrasubstituted olefin of <b>8</b>. The benzylic and allylic C–O bonds were reductively cleaved at the late stage of the syntheses, respectively

Total Synthesis of Epoxyeujindole A

The total synthesis of epoxyeujindole A, a structurally unusual indole diterpenoid isolated from Eupenicillium javanicum, has been accomplished for the first time. The synthesis features a late-stage cationic cyclization strategy, which took advantage of an electron-rich olefinic substrate. The CDE ring system was assembled via an enantioselective conjugate addition/alkylation, a Luche cyclization, and a Nozaki–Hiyama–Kishi reaction. The heavily substituted A ring was constructed through a Suzuki–Miyaura coupling and a cationic cyclization, and the bridged fused B ring was formed through a Prins reaction

TaPP2AbB"-α interacts with TaPP2Aa and TaPP2Ac.

<p>(A) Yeast two-hybrid assay shows interactions between TaPP2AbB"-α and subunits A and C of PP2A. Abbreviations on the left side are: AD, pGADT7 vector; BD, pGBKT7 vector; R, TaPP2AbB"-α; A, TaPP2Aa; C, TaPP2Ac. (B) Interaction of TaPP2AbB"-α with TaPP2Aa revealed by firefly luciferase complementation imaging assay in <i>Nicotiana benthamiana</i> leaves. A, TaPP2Aa; R, TaPP2AbB"-α. Different fluorescence intensity resulted in the color-mixed spots.</p