A tunable nanoplatform of nanogold functionalised with Angiogenin peptides for anti-angiogenic therapy of brain tumours

Angiogenin (ANG), an endogenous protein that plays a key role in cell growth and survival, has been scrutinised here as promising nanomedicine tool for the modulation of pro-/anti-angiogenic processes in brain cancer therapy. Specifically, peptide fragments from the putative cell membrane binding domain (residues 60-68) of the protein were used in this study to obtain peptide-functionalised spherical gold nanoparticles (AuNPs) of about 10 nm and 30 nm in optical and hydrodynamic size, respectively. Different hybrid biointerfaces were fabricated by peptide physical adsorption (Ang60-68) or chemisorption (the cysteine analogous Ang60-68Cys) at the metal nanoparticle surface, and cellular assays were performed in the comparison with ANG-functionalised AuNPs. Cellular treatments were performed both in basal and in copper-supplemented cell culture medium, to scrutinise the synergic effect of the metal, which is another known angiogenic factor. Two brain cell lines were investigated in parallel, namely tumour glioblastoma (A172) and neuron-like differentiated neuroblastoma (d-SH-SY5Y). Results on cell viability/proliferation, cytoskeleton actin, angiogenin translocation and vascular endothelial growth factor (VEGF) release pointed to the promising potentialities of the developed systems as anti-angiogenic tunable nanoplaftforms in cancer cells treatment

Coastal scenic beauty and sensitivity at the balearic islands, spain: Implication of natural and human factors

Coastal areas globally are facing a significant range of environmental stresses, enhanced by climate change-related processes and a continuous increase of human activities. The economic benefits of tourism are well-known for coastal regions, but, very often, conflicts arise between short-term benefits and long-term conservation goals. Among beach user preferences, five parameters of greater importance stand out from the rest, i.e., safety, facilities, water quality, litter and scenery; the latter is the main concern of this study. A coastal scenic evaluation was carried out in the Balearic Islands and focused on two major issues: coastal scenic beauty together with sensitivity to natural processes and human pressure. The archipelago is renowned as a top international coastal tourist destination that receives more than 13.5 million visitors (2019). Impressive landscape diversity makes the Balearics Islands an ideal field for this research. In total, 52 sites, respectively located in Ibiza (11), Formentera (5), Mallorca (18) and Menorca (18), were field-tested. In a first step, coastal scenic beauty was quantified using the coastal scenic evaluation system (CSES) method, based on the evaluation of 26 physical and human parameters, and using weighting matrices parameters and fuzzy logic mathematics. An evaluation index (“D”) was obtained for each site, allowing one to classify them in one of the five scenic classes established by the method. Twenty-nine sites were included in class I, corresponding to extremely attractive sites (CSES), which were mainly observed in Menorca. Several sound measures were proposed to maintain and/or enhance sites’ scenic value. In a second step, scenic sensitivity was evaluated using a novel methodological approach that makes possible the assessment of three different coastal scenic sensitivity indexes (CSSI), i.e., the natural sensitivity index NSI, the human sensitivity index HSI and the total sensitivity index TSI. Future climate change trends and projection of tourism development, studied at municipality scale, were considered as correction factors. All the islands showed places highly sensitive to environmental processes, while sensitivity to human pressure was essentially observed at Ibiza and Mallorca. Thereafter, sites were categorized into one of three sensitive groups established by the methodology. Results obtained are useful in pointing out very sensitive sceneries as well as limiting, preventing and/or anticipating future scenic degradation linked to natural and human issues

Anti-angiogenic and anti-proliferative graphene oxide nanosheets for tumor cell therapy

Graphene oxide (GO) is a bidimensional novel material that exhibits high biocompatibility and angiogenic properties, mostly related to the intracellular formation of reactive oxygen species (ROS). In this work, we set up an experimental methodology for the fabrication of GO@peptide hybrids by the immobilization, via irreversible physical adsorption, of the Ac-(GHHPH)4-NH2 peptide sequence, known to mimic the anti-angiogenic domain of the histidine-proline-rich glycoprotein (HPRG). The anti-proliferative capability of the graphene-peptide hybrids were tested in vitro by viability assays on prostate cancer cells (PC-3 line), human neuroblastoma (SH-SY5Y), and human retinal endothelial cells (primary HREC). The anti-angiogenic response of the two cellular models of angiogenesis, namely endothelial and prostate cancer cells, was scrutinized by prostaglandin E2 (PGE2) release and wound scratch assays, to correlate the activation of inflammatory response upon the cell treatments with the GO@peptide nanocomposites to the cell migration processes. Results showed that the GO@peptide nanoassemblies not only effectively induced toxicity in the prostate cancer cells, but also strongly blocked the cell migration and inhibited the prostaglandin-mediated inflammatory process both in PC-3 and in HRECs. Moreover, the cytotoxic mechanism and the internalization efficiency of the theranostic nanoplatforms, investigated by mitochondrial ROS production analyses and confocal microscopy imaging, unraveled a dose-dependent manifold mechanism of action performed by the hybrid nanoassemblies against the PC-3 cells, with the detection of the GO-characteristic cell wrapping and mitochondrial perturbation. The obtained results pointed out to the very promising potential of the synthetized graphene-based hybrids for cancer therapy

Condensed Matter Theory of Dipolar Quantum Gases

Recent experimental breakthroughs in trapping, cooling and controlling ultracold gases of polar molecules, magnetic and Rydberg atoms have paved the way toward the investigation of highly tunable quantum systems, where anisotropic, long-range dipolar interactions play a prominent role at the many-body level. In this article we review recent theoretical studies concerning the physics of such systems. Starting from a general discussion on interaction design techniques and microscopic Hamiltonians, we provide a summary of recent work focused on many-body properties of dipolar systems, including: weakly interacting Bose gases, weakly interacting Fermi gases, multilayer systems, strongly interacting dipolar gases and dipolar gases in 1D and quasi-1D geometries. Within each of these topics, purely dipolar effects and connections with experimental realizations are emphasized.Comment: Review article; submitted 09/06/2011. 158 pages, 52 figures. This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in Chemical Reviews, copyright American Chemical Society after peer review. To access the final edited and published work, a link will be provided soo

Andalusia, Spain: An Assessment of Coastal Scenery

The 1101 km length of the Andalusian coast (Spain) was assessed for coastal scenery at 45 specific locations. Selected areas covered resort (3), urban (19), village (8), rural (10) and remote (5) bathing areas. Scenery was analyzed for physical and human parameters via 26 selected parameters. These parameters were obtained by interviews of 4500 people on European beaches. Each parameter was assessed via a one-to-five-point attribute scale, which essentially ranged from presence/absence or poor quality (1), to excellent/outstanding (5). Results were subsequently weighted by interviewing 4600 bathing area users (not all 26 parameters have equal weight) and subjected to fuzzy logic mathematics in order to reduce recorder subjectivity. High weighted averages for attributes 4 and 5 (excellent/outstanding) reflected high scenic quality, vice versa for attributes 1 and 2. Sites were classified into five classes ranging from Class 1 sites having top grade scenery to Class 5, poor scenery. Seven sites each were found in Classes 1 and 2; 10 sites each in Classes 3 and 5; 11 sites in Class 4. The finest coastal scenery was found in remote areas whilst urban areas scored mainly as Class 3 or 4. Three out of the ten rural sites had Class 3 and 4 values assigned them whereas the rest scored as Class 1 and 2; village sites invariably had scores within Class 3 and 4. Of the three resort sites investigated, one scored as a Class 1 site, the others as Class 3

Responses of Trapa natans L. floating laminae to high concentrations of manganese.

The present study focuses on the responses of floating laminae of the Mn-tolerant hydrophyte Trapa natans L. to 1 mM Mn and their ability to accumulate the metal. Studies were carried out first on young floating laminae belonging to the second verticil of 30-day-old plants which originated from fruits that had been maintained in a 1 mM Mn-treated environment and again on the young floating laminae after 10 days of further treatment with 1 mM Mn. Mn storing was observed from the first days after germination, but only 10-day-treated laminae showed the capability to hyperaccumulate the element inside specialised cells (>20000 μg/g [dry weight]). Electron microscopy and the Folin-Ciocalteu reaction for phenolics revealed deposits of chelated material inside vacuoles of the first palisade layer and of idioblasts in the spongy tissue. X-ray microanalysis indicated that the deposits were Mn chelated with phenolic compounds. Numerous trichomes were observed at the lower epidermis of 10-day-treated laminae. They were rich in phenolics and characterised by Mn concretions at their base. As they are associated with a high concentration of the metal in culture water and sediments, trichomes may constitute a morphological differentiation for the secretion of Mn-chelating molecules into the culture water, as a probable “avoidance” mechanism. Finally, monitoring of the photosynthetic apparatus showed that photosynthetic function was not impaired, though differences in development occurred

Gene and functional up-regulation of the BCRP/ABCG2 transporter in hepatocellular carcinoma.

The Breast Cancer Resistance Protein (BCRP/ABCG2) is one member of ABC transporters proteins super family responsible of drug resistance. Since data on ABCG2 expression in liver malignances are scanty, here we report the expression of ABCG2 in adult human hepatocellular carcinoma (HCC) in both in vivo and in vitro models with different degree of malignancy.In cell lines derived from human hepatocellular carcinoma, ABCG2 gene expression was assessed by reverse transcription quantitative real time PCR and function by Hoechst 33342 efflux assay; protein content was assessed by SDS-PAGE Western blot.ABCG2 expression was found to be highest in the most undifferentiated cell lines, and this was related with a higher functional activity. ABCG2 expression was sensitive to antineoplastic drugs since exposure to 5 \u3bcM doxorubicin for 24 hours resulted in significant up-regulations of ABCG2 in all cell lines, particularly in those lines with low basal ABCG2 expression (p<0.01). The gene expression was also investigated in 51 adult liver tissues with HCC and related cirrhosis; normal liver tissue was used as control. ABCG2 gene expression was higher in HCC than both cirrhotic paired tissue and normal tissue. This up-regulation was greater (p<0.05) in pathological poorly differentiated grade G3/G4 than in well-differentiated G1/G2 HCC.Our results suggest a correlation of ABCG2 gene expression and differentiation stage both in human and HCC derived cell lines. The rapid up-regulation of ABCG2 to exposure to doxorubicin emphasizes the importance of this transporter in accounting for drug resistance in liver tumors