Observaţii cu privire la fauna epigee din culturile de grâu în condiţiile anului 2017

The observations were made in 2017, in a wheat crop located in the eastern area of Romania, in the county of Vaslui. The material was harvested using the soil traps type Barberand entomological mesh, during which several harvests of the material were made at intervals of 8 to 15 days. From the collected biological material, were retained all the species belonging to the epigee fauna, which were then determined by means of the determiners (Reitter, Panin, etc.). Following the records was established the specific structure from wheat crop, their dynamics and their abundance.The most frequently collected species were: Heteroptera, Hymenoptera, Diptera, Aracnidae, Orthoptera, and of course those belonging to the order of Coleoptera (Pseudophonus rufipes Mull., Dermestes laniarius Illig., Harpalus aeneus F., Harpalus distinendus Duft., Ophonus azureus F., Opatrum sabulosum L., Otiorrhynchus raucus F., Carabus violaceus L., Pseudophonus rufipes, Harpalus aeneus F.şi Amara crenata Payk) etc

Cercetari privind fauna de coleoptere epigee din unele culturi de grau

The study of beetles (Coleoptera) appears as a necessary scientific and practical taking into account, in particular its frequency on the meridian of the Earth, the number of species that they comprise (250,000), the largest of the class Insecta, which groups more than one million species and the many species that cause damage to global agriculture.The material was collected using Barber soil traps, from a wheat culture for consumption in the town of Delesti, Vaslui County.The observations were made in 2017, the material was collected from May to October. The material was harvested at intervals between 8 and 17 days, with a total of 5 harvests.The species most frequently collected were Opatrum sabulosum L., Otiorrhynchus raucus F., cupreus Carabus L., Pseudophonus rufipes, Harpalus aeneus Amara aenea F.şi Payk

Anatomical, histomorphological, and molecular classification of cholangiocarcinoma

Cholangiocarcinoma constitutes a heterogeneous group of malignancies that can emerge at any point of the biliary tree. Cholangiocarcinoma is classified into intrahepatic, perihilar and distal based on its anatomical location. Histologically, conventional perihilar/distal cholangiocarcinomas are mucin-producing adenocarcinomas or papillary tumours; intrahepatic cholangiocarcinomas are more heterogeneous and can be sub-classified according to the level or size of the displayed bile duct. Cholangiocarcinoma develops through multistep carcinogenesis and is preceded by dysplastic and in situ lesions. Definition and clinical significance of precursor lesions, including biliary intraepithelial neoplasia, intraductal papillary neoplasms of the bile duct, intraductal tubulopapillary neoplasms and mucinous cystic neoplasm, are discussed in this review. A main challenge in diagnosing cholangiocarcinoma is the fact that tumour tissue for histological examination is difficult to obtain. Thus, a major clinical obstacle is the establishment of the correct diagnosis at a tumour stage that is amenable to surgery which still represents the only curable therapeutic option. Current standards, methodology and criteria for diagnosis are discussed. Cholangiocarcinoma represents a heterogeneous tumour with regard to molecular alterations. In intrahepatic subtype, mainly two distinctive morpho-molecular groups can currently be discriminated. Large-duct type intrahepatic cholangiocarcinoma shows a high mutation frequency of oncogenes and tumour suppressor genes, such as KRAS and TP53 while Isocitrate Dehydrogenase 1/2 mutations and Fibroblast Growth Factor Receptor 2-fusions are typically seen in small-duct type tumours. It is most important to ensure the separation of the given anatomical subtypes and to search for distinct subgroups within the subtypes on a molecular and morphological basis

Cercetări privind entomofauna de coleoptere (coleoptera) din unele culturi agricole din N-E Moldovei

The purpose of these research is to identify the useful entomofauna from the agricultural crops which have been taken for the study: maize and cabbage. The researches were carried out in the Adamachi station which belongs to “Ion Ionescu de la Brad” University of Iasi, Romania. The biological material was sampled by means of the Barber soil traps from 3rd of June until 13th of September 2016. In total, 15 samples were made for every crop. The traps were placed every five in a row. To prevent the maceration of insects, a conservative liquid was used (water+ washing powder) in proportion of 16%. After finishing the experiments, the insects were bringing to the laboratory of Entomology in order to be counted and determined. From the Coleoptera order, the most dominant species are:-within the crop of maize: Amara aenea (Carabidae)-5 samples; Coccinella septempunctata (Coccinellidae)-15 samples;-within the crop of cabbage:Brachinus crepitans(Carabidae)-9 samples

Clinical and pathological characteristics of 306 pancreatic adenocarcinoma patients.

<p>Clinical and pathological characteristics of 306 pancreatic adenocarcinoma patients.</p

Kaplan-Meier survival curve for 306 pancreatic ductal adenocarcinoma patients.

<p>Kaplan-Meier survival curve for 306 pancreatic ductal adenocarcinoma patients.</p

Serial H&E (A,C) and BAP1 IHC (B, D) stained sections of pancreatic ductal adenocarcinomas which demonstrate positive immunohistochemical staining for BAP1.

<p>All the neoplastic and non-neoplastic cell demonstrate diffuse strong nuclear staining (Original magnification A,B 100x, C,D 400x).</p

Haematoxylin & eosin (A, C) and BAP1 immunohistochemistry (B, D) stained sections from the sole pancreatic ductal adenocarcinoma patient who expressed loss of nuclear BAP1.

<p>In this case, the exocrine cells (solid arrows) clearly lacked the brown BAP1 staining, and the non-neoplastic endothelial cells serve as the internal positive controls (hollow arrows). Magnifications: A, B 100x; C, D 400x.</p

Genomic profiling of malignant peritoneal mesothelioma reveals recurrent alterations in epigenetic regulatory genes BAP1, SETD2, and DDX3X

Malignant mesothelioma is a rare cancer that arises from the mesothelial cells that line the pleural cavity and less commonly from the peritoneal lining of the abdomen and pelvis. Most pleural mesotheliomas arise in patients with a history of asbestos exposure, whereas the association of peritoneal mesotheliomas with exposure to asbestos and other potential carcinogens is less clear, suggesting that the genetic alterations which drive malignant peritoneal mesothelioma may be unique from those in pleural mesothelioma. Treatment options for all malignant mesotheliomas are currently limited, with no known targeted therapies available. To better understand the molecular pathogenesis of malignant peritoneal mesothelioma, we sequenced 510 cancer-related genes in 13 patients with malignant mesothelioma arising in the peritoneal cavity. The most frequent genetic alteration was biallelic inactivation of the BAP1 gene, which occurred in 9/13 cases, with an additional 2 cases demonstrating monoallelic loss of BAP1. All 11 of these cases demonstrated loss of BAP1 nuclear staining by immunohistochemistry, whereas the 2 tumors without BAP1 alteration and all 42 cases of histologic mimics in peritoneum (8 multilocular peritoneal inclusion cyst, 6 well-differentiated papillary mesothelioma of the peritoneum, 16 adenomatoid tumor, and 12 low-grade serous carcinoma of the ovary) demonstrated intact BAP1 nuclear staining. Additional recurrently mutated genes in this cohort of malignant peritoneal mesotheliomas included NF2 (3/13), SETD2 (2/13), and DDX3X (2/13). While these genes are known to be recurrently mutated in pleural mesotheliomas, the frequencies are distinct in peritoneal mesotheliomas, with nearly 85% of peritoneal tumors harboring BAP1 alterations versus only 20-30% of pleural tumors. Together, these findings demonstrate the importance of epigenetic modifiers including BAP1, SETD2, and DDX3X in mesothelial tumorigenesis and suggest opportunities for targeted therapies