3,513 research outputs found

    Mechanical and Vascular Cues Synergistically Enhance Osteogenesis in Human Mesenchymal Stem Cells

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    Development and maintenance of a vascular network are critical for bone growth and homeostasis; strategies that promote vascular function are critical for clinical success of tissue-engineered bone constructs. Co-culture of endothelial cells (ECs) with mesenchymal stem cells (MSCs) and exposure to 10% cyclic tensile strain have both been shown to regulate osteogenesis in isolation, but potential synergistic effects have yet to be explored. The objective of this study was to expose an MSC-EC co-culture to 10% cyclic tensile strain to examine the role of this mechanical stimulus on MSC-EC behavior. We hypothesized that paracrine signaling from ECs would stimulate osteogenesis of MSCs, and exposure to 10% cyclic tensile strain would enhance this anabolic signal. Human umbilical vein ECs and human bone marrow-derived MSCs were either monocultured or co-cultured at a 1:1 ratio in a mixed osteo/angiogenic medium, exposed to 10% cyclic tensile strain at 1 Hz for 4 h/day for 2 weeks, and biochemically and histologically analyzed for endothelial and osteogenic markers. While neither 10% cyclic tensile strain nor co-culture alone had a significant effect on osteogenesis, the concurrent application of strain to an MSC-EC co-culture resulted in a significant increase in calcium accretion and mineral deposition, suggesting that co-culture and strain synergistically enhance osteogenesis. Neither co-culture, 10% cyclic tensile strain, nor a combination of these stimuli affected endothelial markers, indicating that the endothelial phenotype remained stable, but unresponsive to the stimuli evaluated in this study. This study is the first to investigate the role of cyclic tensile strain on the complex interplay between ECs and MSCs in co-culture. The results of this study provide key insights into the synergistic effects of 10% cyclic tensile strain and co-culture on osteogenesis. Understanding mechanobiological factors affecting MSC-EC crosstalk will help enhance strategies for creating vascularized tissues in tissue engineering and regenerative medicine

    Coherent electronic transfer in quantum dot systems using adiabatic passage

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    We describe a scheme for using an all-electrical, rapid, adiabatic population transfer between two spatially separated dots in a triple-quantum dot system. The electron spends no time in the middle dot and does not change its energy during the transfer process. Although a coherent population transfer method, this scheme may well prove useful in incoherent electronic computation (for example quantum-dot cellular automata) where it may provide a coherent advantage to an otherwise incoherent device. It can also be thought of as a limiting case of type II quantum computing, where sufficient coherence exists for a single gate operation, but not for the preservation of superpositions after the operation. We extend our analysis to the case of many intervening dots and address the issue of transporting quantum information through a multi-dot system.Comment: Replaced with (approximately) the published versio

    The Clustering of Massive Halos

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    The clustering properties of dark matter halos are a firm prediction of modern theories of structure formation. We use two large volume, high-resolution N-body simulations to study how the correlation function of massive dark matter halos depends upon their mass and formation history. We find that halos with the lowest concentrations are presently more clustered than those of higher concentration, the size of the effect increasing with halo mass; this agrees with trends found in studies of lower mass halos. The clustering dependence on other characterizations of the full mass accretion history appears weaker than the effect with concentration. Using the integrated correlation function, marked correlation functions, and a power-law fit to the correlation function, we find evidence that halos which have recently undergone a major merger or a large mass gain have slightly enhanced clustering relative to a randomly chosen population with the same mass distribution.Comment: 10 pages, 8 figures; text improved, references and one figure added; accepted for publication in Ap

    Close Pairs as Proxies for Galaxy Cluster Mergers

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    Galaxy cluster merger statistics are an important component in understanding the formation of large-scale structure. Unfortunately, it is difficult to study merger properties and evolution directly because the identification of cluster mergers in observations is problematic. We use large N-body simulations to study the statistical properties of massive halo mergers, specifically investigating the utility of close halo pairs as proxies for mergers. We examine the relationship between pairs and mergers for a wide range of merger timescales, halo masses, and redshifts (0<z<1). We also quantify the utility of pairs in measuring merger bias. While pairs at very small separations will reliably merge, these constitute a small fraction of the total merger population. Thus, pairs do not provide a reliable direct proxy to the total merger population. We do find an intriguing universality in the relation between close pairs and mergers, which in principle could allow for an estimate of the statistical merger rate from the pair fraction within a scaled separation, but including the effects of redshift space distortions strongly degrades this relation. We find similar behavior for galaxy-mass halos, making our results applicable to field galaxy mergers at high redshift. We investigate how the halo merger rate can be statistically described by the halo mass function via the merger kernel (coagulation), finding an interesting environmental dependence of merging: halos within the mass resolution of our simulations merge less efficiently in overdense environments. Specifically, halo pairs with separations less than a few Mpc/h are more likely to merge in underdense environments; at larger separations, pairs are more likely to merge in overdense environments.Comment: 12 pages, 9 figures; Accepted for publication in ApJ. Significant additions to text and two figures changed. Added new findings on the universality of pair mergers and added analysis of the effect of FoF linking length on halo merger

    Statistics of Magnification Perturbations by Substructure in the Cold Dark Matter Cosmological Model

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    We study the statistical properties of magnification perturbations by substructures in strong lensed systems using linear perturbation theory and an analytical substructure model including tidal truncation and a continuous substructure mass spectrum. We demonstrate that magnification perturbations are dominated by perturbers found within a tidal radius of an image, and that sizable magnification perturbations may arise from small, coherent contributions from several substructures within the lens halo. We find that the root-mean-square (rms) fluctuation of the magnification perturbation is 10% to 20% and both the average and rms perturbations are sensitive to the mass spectrum and density profile of the perturbers. Interestingly, we find that relative to a smooth model of the same mass, the average magnification in clumpy models is lower (higher) than that in smooth models for positive (negative) parity images. This is opposite from what is observed if one assumes that the image magnification predicted by the best-fit smooth model of a lens is a good proxy for what the observed magnification would have been if substructures were absent. While it is possible for this discrepancy to be resolved via nonlinear perturbers, we argue that a more likely explanation is that the assumption that the best-fit lens model is a good proxy for the magnification in the absence of substructure is not correct. We conclude that a better theoretical understanding of the predicted statistical properties of magnification perturbations by CDM substructure is needed in order to affirm that CDM substructures have been unambiguously detected.Comment: ApJ accepted, minor change

    Halo Substructure and the Power Spectrum

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    In this proceeding, we present the results of a semi-analytic study of CDM substructure as a function of the primordial power spectrum. We apply our method to several tilted models in the LCDM framework with n=0.85-1.1, sigma_8=0.65-1.2 when COBE normalized. We also study a more extreme, warm dark matter-like spectrum that is sharply truncated below a scale of 10^10 h^-1 Msun. We show that the mass fraction of halo substructure is not a strong function of spectral slope, so it likely will be difficult to constrain tilt using flux ratios of gravitationally lensed quasars. On the positive side, all of our CDM-type models yield projected mass fractions in good agreement with strong lensing estimates: f \sim 1.5% at M \sim 10^8 Msun. The truncated model produces a significantly smaller fraction, f \lsim 0.3%, suggesting that warm dark matter-like spectra may be distinguished from CDM spectra using lensing. We also discuss the issue of dwarf satellite abundances, with emphasis on the cosmological dependence of the map between the observed central velocity dispersion of Milky Way satellites and the maximum circular velocities of their host halos. In agreement with earlier work, we find that standard LCDM over-predicts the estimated count of Milky Way satellites at fixed Vmax by an order of magnitude, but tilted models do better because subhalos are less concentrated. Interestingly, under the assumption that dwarfs have isotropic velocity dispersion tensors, models with significantly tilted spectra (n \lsim 0.85, sigma_8 \lsim 0.7) may under-predict the number of large Milky Way satellites with Vmax \gsim 40 km/s.Comment: 5 pages, 2 figures. Poster contribution to the 13th Annual Astrophysics Conference in Maryland, The Emergence of Cosmic Structur

    Dark Matter and Stellar Mass in the Luminous Regions of Disk Galaxies

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    We investigate the correlations among stellar mass (M_*), disk scale length (R_d), and rotation velocity at 2.2 disk scale lengths (V_2.2) for a sample of 81 disk-dominated galaxies (disk/total >= 0.9) selected from the SDSS. We measure V_2.2 from long-slit H-alpha rotation curves and infer M_* from galaxy i-band luminosities (L_i) and g-r colors. We find logarithmic slopes of 2.60+/-0.13 and 3.05+/-0.12 for the L_i-V_2.2 and M_*-V_2.2 relations, somewhat shallower than most previous studies, with intrinsic scatter of 0.13 dex and 0.16 dex. Our direct estimates of the total-to-stellar mass ratio within 2.2R_d, assuming a Kroupa IMF, yield a median ratio of 2.4 for M_*>10^10 Msun and 4.4 for M_*=10^9-10^10 Msun, with large scatter at a given M_* and R_d. The typical ratio of the rotation speed predicted for the stellar disk alone to the observed rotation speed at 2.2R_d is ~0.65. The distribution of R_d at fixed M_* is broad, but we find no correlation between disk size and the residual from the M_*-V_2.2 relation, implying that this relation is an approximately edge-on view of the disk galaxy fundamental plane. Independent of the assumed IMF, this result implies that stellar disks do not, on average, dominate the mass within 2.2R_d. We discuss our results in the context of infall models of disk formation in cold dark matter halos. A model with a disk-to-halo mass ratio m_d=0.05 provides a reasonable match to the R_d-M_* distribution for spin parameters \lambda ranging from ~0.04-0.08, and it yields a reasonable match to the mean M_*-V_2.2 relation. A model with m_d=0.1 predicts overly strong correlations between disk size and M_*-V_2.2 residual. Explaining the wide range of halo-to-disk mass ratios within 2.2R_d requires significant scatter in m_d values, with systematically lower m_d for galaxies with lower M∗M_*.Comment: 18 pages, 2 tables, 7 figures, Accepted to ApJ, Table 1 updated, otherwise minor change
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