Recreating Bioenergetic Elements In Protein Maquettes

In all organisms, protein-mediated electron transfers underlie energy metabolism and countless other critical metabolic pathways. Deciphering the factors governing electron transfers offers enormous practical value, including finding reliable guidelines for metabolic engineering to produce clean solar fuels. Protein maquettes provide simple, flexible scaffolds to study biological electron transfer processes. The maquette approach to protein design builds man-made oxidoreductases from first principles with minimal reference to natural protein sequences. This scheme has produced impressive in vitro and in vivo results to replicate the functions of natural oxidoreductases using simple, straightforward α-helical bundles. The redox function of maquettes has so far been limited to electron transfers within a single water-soluble molecule. This work extends the maquette project to examine electron transfers A) between diffusing redox partners and B) in amphiphilic proteins. Nature employs complimentary electrostatic surfaces to bring diffusing redox proteins together, and a series of maquettes of varying surface charge demonstrate that the same principles apply to flexible manmade proteins. A heme-binding maquette of complimentary surface charge is shown to reduce natural cytochrome c at physiological rates, while a maquette variant similar in charge to cyt c shows a far weaker interaction. The ionic strength dependence of these interactions is shown to be broadly similar to that in natural proteins. This work also presents the first intra-protein redox function in a de novo amphiphilic protein and describes progress toward transmembrane electron transfer analogous to that in the cytochrome bc1 complex. These functional achievements are remarkable given that the proteins are designed from first principles without atomically resolved structures, and they hold promise for future efforts in applying artificial proteins to new metabolic pathways

College baseball players’ perception of their team climate and mental health

The purpose of this study, grounded in the Achievement Goal Perspective Theory (AGPT) and a Caring framework, was to examine the extent that collegiate baseball players’ perceptions of their team climate (i.e., caring, task-, and ego-involving climate) predict their mental well-being. Baseball players (N = 127) completed measures assessing perceptions of team climate (Perceived Motivational Climate in Sport Questionnaire, Caring Climate Scale) and mental well-being (World Health Organization-Five Well-Being Index). Spearman correlation analysis revealed a positive relationship between the caring and task-involving climate scales, and mental well-being, respectively. In a full entry model linear regression, caring climate emerged as a positive predictor of mental well-being, explaining 25% of the variance of athletes’ mental well-being scores. The findings suggest that encouraging coaches to foster a caring and task-involving climate might assist in enhancing collegiate athletes’ mental well-being. Athletes perceiving a high caring and task-involving environment are more likely to experience improved mental well-being.Our research was supported in part by the Wu Tsai Foundation

Visual control of flight speed in Drosophila melanogaster

Flight control in insects depends on self-induced image motion (optic flow), which the visual system must process to generate appropriate corrective steering maneuvers. Classic experiments in tethered insects applied rigorous system identification techniques for the analysis of turning reactions in the presence of rotating pattern stimuli delivered in open-loop. However, the functional relevance of these measurements for visual free-flight control remains equivocal due to the largely unknown effects of the highly constrained experimental conditions. To perform a systems analysis of the visual flight speed response under free-flight conditions, we implemented a `one-parameter open-loop' paradigm using `TrackFly' in a wind tunnel equipped with real-time tracking and virtual reality display technology. Upwind flying flies were stimulated with sine gratings of varying temporal and spatial frequencies, and the resulting speed responses were measured from the resulting flight speed reactions. To control flight speed, the visual system of the fruit fly extracts linear pattern velocity robustly over a broad range of spatio–temporal frequencies. The speed signal is used for a proportional control of flight speed within locomotor limits. The extraction of pattern velocity over a broad spatio–temporal frequency range may require more sophisticated motion processing mechanisms than those identified in flies so far. In Drosophila, the neuromotor pathways underlying flight speed control may be suitably explored by applying advanced genetic techniques, for which our data can serve as a baseline. Finally, the high-level control principles identified in the fly can be meaningfully transferred into a robotic context, such as for the robust and efficient control of autonomous flying micro air vehicles

Fundamentals of mercury oxidation in flue gas

ReportThis report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof

Role of OH-stretch/torsion coupling and quantum yield effects in the first OH overtone spectrum of cis-cis HOONO

A joint theoretical and experimental investigation is undertaken to study the effects of OH-stretch/HOON torsion coupling and of quantum yield on the previously reported first overtone action spectrum of cis-cis HOONO (peroxynitrous acid). The minimum energy path along the HOON dihedral angle is computed at the coupled cluster singles and doubles with perturbative triples level with correlation consistent polarized quadruple zeta basis set, at the structure optimized using the triple zeta basis set (CCSD(T)/cc-pVQZ//CCSD(T)/cc-pVTZ). The two-dimensional ab initio potential energy and dipole moment surfaces for cis-cis HOONO are calculated as functions of the HOON torsion and OH bond length about the minimum energy path at the CCSD(T)/cc-pVTZ and QCISD/AUG-cc-pVTZ (QCISD—quadratic configuration interaction with single and double excitation and AUG-augmented with diffuse functions) level of theory/basis, respectively. The OH-stretch vibration depends strongly on the torsional angle, and the torsional potential possesses a broad shelf at ~90°, the cis-perp conformation. The calculated electronic energies and dipoles are fit to simple functional forms and absorption spectra in the region of the OH fundamental and first overtone are calculated from these surfaces. While the experimental and calculated spectra of the OH fundamental band are in good agreement, significant differences in the intensity patterns are observed between the calculated absorption spectrum and the measured action spectrum in the 2nuOH region. These differences are attributed to the fact that several of the experimentally accessible states do not have sufficient energy to dissociate to OH+NO2 and therefore are not detectable in an action spectrum. Scaling of the intensities of transitions to these states, assuming D0=82.0 kJ/mol, is shown to produce a spectrum that is in good agreement with the measured action spectrum. Based on this agreement, we assign two of the features in the spectrum to Delta n=0 transitions (where n is the HOON torsion quantum number) that are blue shifted relative to the origin band, while the large peak near 7000 cm^–1 is assigned to a series of Delta n=+1 transitions, with predominant contributions from torsionally excited states with substantial cis-perp character. The direct absorption spectrum of cis-cis HOONO (6300–6850 cm^–1) is recorded by cavity ringdown spectroscopy in a discharge flow cell. A single band of HOONO is observed at 6370 cm^–1 and is assigned as the origin of the first OH overtone of cis-cis HOONO. These results imply that the origin band is suppressed by over an order of magnitude in the action spectrum, due to a reduced quantum yield. The striking differences between absorption and action spectra are correctly predicted by the calculations

Idahoans\u27 Attitudes About COVID-19 2020

The Sixth Annual Idaho Public Policy Survey was conducted November 29th–December 3rd, 2020, and surveyed 1,000 adults over the age of 18 who currently live in Idaho. The sample is designed to be representative of the population of the state both geographically and demographically. This was a mixed-mode survey which contacted by phone (58%), online (32%), and via text message (10%). The goal of using multiple means to contact respondents is to increase our coverage of the population to people who may not respond to traditional phone surveys. The survey addresses a variety of issues facing Idaho including growth, education, taxes, and criminal justice, but this brief will focus on attitudes regarding the COVID-19 pandemic. The survey has a simple random sampling margin of error of +/- 3.1% and was conducted by GS Strategy Group

Performance Motion Analysis Unable to Predict Running-Related Injury in Collegiate Distance Runners

ABSTRACT Purpose: Running-related injury (RRI) is common among competitive collegiate distance runners who participate in the sport of cross country and long distance track and field. Many factors contribute to RRI. Therefore, the purpose of this study was to determine if a 3D motion capture system’s performance motion analysis (PMA) report is capable of identifying factors predictive of RRI among collegiate distance runners during a cross country season. Methods: Thirty-one collegiate cross country runners (17 male, 14 female, mean age = 20.5 ± 1.4 years) gave their consent to participate in the investigation. Subjects were screened in the motion capture system and provided with PMA reports assessing their movement quality using several variables (composite score, power, strength, dysfunction, and vulnerability, based on measurements of 192 kinetic and kinematic variables). The athletes were then monitored throughout their 13-week competitive season for incidence of RRI. At the end of the season, participants were sorted into injured (n=17) and uninjured (n=14) groups. Injury was defined as appearing on the team injury report as missing or being limited in practice or competition for a week or more, in accordance with prior RRI research. Each sex was also separated into groups based on injury status. Results: Independent samples t-tests (pConclusion: The findings identified in this prospective study suggest that the movement screen was unable to identify runners at risk of injury. Future investigations isolating lower extremity movement characteristics in runners may prove more effective at predicting RRI

Growth in the Treasure Valley in 2020

The Sixth Annual Idaho Public Policy Survey was conducted November 29-December 3, 2020, and surveyed 1,000 adults over the age of 18 who currently live in Idaho. The Treasure Valley was oversampled to survey 750 residents of Ada (66%), Canyon (27%), Gem (4%), Boise (4%), and Owyhee (1%) counties. The results here focus on this Treasure Valley sample. The sample is designed to be representative of the population of the region both geographically and demographically. This was a mixed-mode survey which contacted by phone (58%), online (32%), and via text message (10%). The goal of using multiple means to contact respondents is to increase our coverage of the population to people who may not respond to traditional phone surveys. The survey has a simple random sampling margin of error of +/- 3.6% for the Treasure Valley, and was conducted by GS Strategy Group

Sixth Annual Idaho Public Policy Survey

The Sixth Annual Idaho Public Policy Survey was conducted November 29-December 3, 2020 and surveyed 1,000 adults who currently live in Idaho. The sample is representative of the state’s population both geographically and demographically. Survey responses were collected by phone (58%), online (32%), and via text message (10%). The survey addresses a variety of issues facing Idaho including growth, education, taxes, criminal justice, and the COVID-19 pandemic. The survey has a simple random sampling margin of error of +/– 3.1% and was conducted by GS Strategy Group

Appropriate Loads for Peak-Power During Resisted Sprinting on a Non-Motorized Treadmill

The purpose of this study was to determine the load which allows the highest peak power for resisted sprinting on a non-motorized treadmill and to determine if other variables are related to individual differences. Thirty college students were tested for vertical jump, vertical jump peak and mean power, 10 m sprint, 20 m sprint, leg press 1 RM, leg press 1 RM relative to body weight, leg press 1 RM relative to lean body mass, leg press 1 RM power, and leg press power at 80% of 1 RM. Participants performed eight resisted sprints on a non-motorized treadmill, with increasing relative loads expressed as percent of body weight. Sprint peak power was measured for each load. Pearson correlations were used to determine if relationships between the sprint peak power load and the other variables were significant. The sprint peak power load had a mode of 35% with 73% of all participants having a relative sprint peak power load between 25-35%. Significant correlations occurred between sprint peak power load and body weight, lean body mass, vertical jump peak and mean power, leg press 1 RM, leg press 1 RM relative to lean body mass, leg press 1 RM power, and leg press power at 80% of 1 RM (r = 0.44, 0.43, 0.39, 0.37, 0.47, 0.39, 0.46, and 0.47, respectively). Larger, stronger, more powerful athletes produced peak power at a higher relative load during resisted sprinting on a nonmotorized treadmill