3 research outputs found
Real-Time Monitoring of Cellular Bioenergetics with a Multianalyte Screen-Printed Electrode
Real-Time Monitoring of Cellular Bioenergetics with a Multianalyte Screen-Printed Electrode
Real-time monitoring of changes to
cellular bioenergetics can provide
new insights into mechanisms of action for disease and toxicity. This
work describes the development of a multianalyte screen-printed electrode
for the detection of analytes central to cellular bioenergetics: glucose,
lactate, oxygen, and pH. Platinum screen-printed electrodes were designed
in-house and printed by Pine Research Instrumentation. Electrochemical
plating techniques were used to form quasi-reference and pH electrodes.
A Dimatix materials inkjet printer was used to deposit enzyme and
polymer films to form sensors for glucose, lactate, and oxygen. These
sensors were evaluated in bulk solution and microfluidic environments,
and they were found to behave reproducibly and possess a lifetime
of up to 6 weeks. Linear ranges and limits of detection for enzyme-based
sensors were found to have an inverse relationship with enzyme loading,
and iridium oxide pH sensors were found to have super-Nernstian responses.
Preliminary measurements where the sensor was enclosed within a microfluidic
channel with RAW 264.7 macrophages were performed to demonstrate the
sensors’ capabilities for performing real-time microphysiometry
measurements