252 research outputs found
Performance Characterization of Complex Fuel Port Geometries for Hybrid Rocket Fuel Grains
This research investigated the 3D printing and burning of fuel grains with complex geometry and the development of software capable of modeling and predicting the regression of a cross-section of these complex fuel grains. The software developed did predict the geometry to a fair degree of accuracy, especially when enhanced corner rounding was turned on. The model does have some drawbacks, notably being relatively slow, and does not perfectly predict the regression. If corner rounding is turned off, however, the model does become much faster; although less accurate, this method does still predict a relatively accurate resulting burn geometry, and is fast enough to be used for performance-tuning or genetic algorithms. In addition to the modeling method, preliminary investigations into the burning behavior of fuel grains with a helical flow path were performed. The helix fuel grains have a regression rate of nearly 3 times that of any other fuel grain geometry, primarily due to the enhancement of the friction coefficient between the flow and flow path
Phosphorus transport in the Berg River, Western Cape
Includes bibliographical references.The objective of this investigation was to develop a dynamic phosphorus export model that describes the transportation of phosphorus through the Berg River drainage basin. Such a model had to consider (1) export of phosphorus from nonpoint (diffuse) sources via surface and subsurface drainage, and from point sources such as wastewater treatment discharges, (2) transportation of phosphorus in the water prism along the river channel, taking account of removal and remobilization of phosphorus from and to the water column, and transportation of phosphorus in the bed load
Remote Toehold: A Mechanism for Flexible Control of DNA Hybridization Kinetics
Hybridization of DNA strands can be used to build molecular devices, and control of the kinetics of DNA hybridization is a crucial element
in the design and construction of functional and autonomous devices.
Toehold-mediated strand displacement has proved to be a powerful
mechanism that allows programmable control of DNA hybridization. So
far, attempts to control hybridization kinetics have mainly focused on
the length and binding strength of toehold sequences. Here we show that
insertion of a spacer between the toehold and displacement domains
provides additional control: modulation of the nature and length of the
spacer can be used to control strand-displacement rates over at least 3
orders of magnitude. We apply this mechanism to operate displacement
reactions in potentially useful kinetic regimes: the kinetic
proofreading and concentration-robust regimes
Modelling DNA Origami Self-Assembly at the Domain Level
We present a modelling framework, and basic model parameterization, for the
study of DNA origami folding at the level of DNA domains. Our approach is
explicitly kinetic and does not assume a specific folding pathway. The binding
of each staple is associated with a free-energy change that depends on staple
sequence, the possibility of coaxial stacking with neighbouring domains, and
the entropic cost of constraining the scaffold by inserting staple crossovers.
A rigorous thermodynamic model is difficult to implement as a result of the
complex, multiply connected geometry of the scaffold: we present a solution to
this problem for planar origami. Coaxial stacking and entropic terms,
particularly when loop closure exponents are taken to be larger than those for
ideal chains, introduce interactions between staples. These cooperative
interactions lead to the prediction of sharp assembly transitions with notable
hysteresis that are consistent with experimental observations. We show that the
model reproduces the experimentally observed consequences of reducing staple
concentration, accelerated cooling and absent staples. We also present a
simpler methodology that gives consistent results and can be used to study a
wider range of systems including non-planar origami
Dimensions and Global Twist of Single-Layer DNA Origami Measured by Small-Angle X-ray Scattering
The
rational design of complementary DNA sequences can be used
to create nanostructures that self-assemble with nanometer precision.
DNA nanostructures have been imaged by atomic force microscopy and
electron microscopy. Small-angle X-ray scattering (SAXS) provides
complementary structural information on the ensemble-averaged state
of DNA nanostructures in solution. Here we demonstrate that SAXS can
distinguish between different single-layer DNA origami tiles that
look identical when immobilized on a mica surface and imaged with
atomic force microscopy. We use SAXS to quantify the magnitude of
global twist of DNA origami tiles with different crossover periodicities:
these measurements highlight the extreme structural sensitivity of
single-layer origami to the location of strand crossovers. We also
use SAXS to quantify the distance between pairs of gold nanoparticles
tethered to specific locations on a DNA origami tile and use this
method to measure the overall dimensions and geometry of the DNA nanostructure
in solution. Finally, we use indirect Fourier methods, which have
long been used for the interpretation of SAXS data from biomolecules,
to measure the distance between DNA helix pairs in a DNA origami nanotube.
Together, these results provide important methodological advances
in the use of SAXS to analyze DNA nanostructures in solution and insights
into the structures of single-layer DNA origami
A DNA Network as an Information Processing System
Biomolecular systems that can process information are sought for computational applications, because of their potential for parallelism and miniaturization and because their biocompatibility also makes them suitable for future biomedical applications. DNA has been used to design machines, motors, finite automata, logic gates, reaction networks and logic programs, amongst many other structures and dynamic behaviours. Here we design and program a synthetic DNA network to implement computational paradigms abstracted from cellular regulatory networks. These show information processing properties that are desirable in artificial, engineered molecular systems, including robustness of the output in relation to different sources of variation. We show the results of numerical simulations of the dynamic behaviour of the network and preliminary experimental analysis of its main components
A new architecture for DNA-templated synthesis in which abasic sites protect reactants from degradation
The synthesis of artificial sequence-defined polymers that match and extend the functionality of proteins is an important goal in materials science. One way of achieving this is to program a sequence of chemical reactions between precursor building blocks by means of attached oligonucleotide adapters. However, hydrolysis of the reactive building blocks has so far limited the length and yield of product that can be obtained using DNA-templated reactions. Here, we report an architecture for DNA-templated synthesis in which reactants are tethered at internal abasic sites on opposite strands of a DNA duplex. We show that an abasic site within a DNA duplex can protect a nearby thioester from degradation, significantly increasing the yield of a DNA-templated reaction. This protective effect has the potential to overcome the challenges associated with programmable sequence-controlled synthesis of long non-natural polymers by extending the lifetime of the reactive building blocks
A New Architecture for DNA‐Templated Synthesis in Which Abasic Sites Protect Reactants from Degradation
The synthesis of artificial sequence‐defined polymers that match and extend the functionality of proteins is an important goal in materials science. One way of achieving this is to program a sequence of chemical reactions between precursor building blocks by means of attached oligonucleotide adapters. However, hydrolysis of the reactive building blocks has so far limited the length and yield of product that can be obtained using DNA‐templated reactions. Here, we report an architecture for DNA‐templated synthesis in which reactants are tethered at internal abasic sites on opposite strands of a DNA duplex. We show that an abasic site within a DNA duplex can protect a nearby thioester from degradation, significantly increasing the yield of a DNA‐templated reaction. This protective effect has the potential to overcome the challenges associated with programmable, sequence‐controlled synthesis of long non‐natural polymers by extending the lifetime of the reactive building blocks
A New Architecture for DNA‐Templated Synthesis in Which Abasic Sites Protect Reactants from Degradation
The synthesis of artificial sequence‐defined polymers that match and extend the functionality of proteins is an important goal in materials science. One way of achieving this is to program a sequence of chemical reactions between precursor building blocks by means of attached oligonucleotide adapters. However, hydrolysis of the reactive building blocks has so far limited the length and yield of product that can be obtained using DNA‐templated reactions. Here, we report an architecture for DNA‐templated synthesis in which reactants are tethered at internal abasic sites on opposite strands of a DNA duplex. We show that an abasic site within a DNA duplex can protect a nearby thioester from degradation, significantly increasing the yield of a DNA‐templated reaction. This protective effect has the potential to overcome the challenges associated with programmable, sequence‐controlled synthesis of long non‐natural polymers by extending the lifetime of the reactive building blocks
A modular RNA delivery system comprising spherical nucleic acids built on endosome-escaping polymeric nanoparticles
Nucleic acid therapeutics require delivery systems to reach their targets. Key challenges to be overcome include avoidance of accumulation in cells of the mononuclear phagocyte system and escape from the endosomal pathway. Spherical nucleic acids (SNAs), in which a gold nanoparticle supports a corona of oligonucleotides, are promising carriers for nucleic acids with valuable properties including nuclease resistance, sequence-specific loading and control of receptor-mediated endocytosis. However, SNAs accumulate in the endosomal pathway and are thus vulnerable to lysosomal degradation or recycling exocytosis. Here, an alternative SNA core based on diblock copolymer PMPC25–PDPA72 is investigated. This pH-sensitive polymer self-assembles into vesicles with an intrinsic ability to escape endosomes via osmotic shock triggered by acidification-induced disassembly. DNA oligos conjugated to PMPC25–PDPA72 molecules form vesicles, or polymersomes, with DNA coronae on luminal and external surfaces. Nucleic acid cargoes or nucleic acid-tagged targeting moieties can be attached by hybridization to the coronal DNA. These polymeric SNAs are used to deliver siRNA duplexes against C9orf72, a genetic target with therapeutic potential for amyotrophic lateral sclerosis, to motor neuron-like cells. By attaching a neuron-specific targeting peptide to the PSNA corona, effective knock-down is achieved at doses of 2 particles per cell
- …