Grain productive efficiency of soybean plants under lactofen application

Adverse factors cause a decrease in the productive potential of crops. For soybean [(Glycine max (L.) Merrill], the excessive growth is a factor that results in plants with low effective efficiency. Thus, plants with an architecture that favours greater interception of solar radiation and deposition of pesticides tend to be more productive. The objective of this study is to evaluate the different application of lactofen, which is used as a growth inhibitor, improve the productive efficiency of soybeans by increasing the biological activity of the leaves. The study was conducted in the field with soybean cultivars NA 5909 RG and BMX Potência RR. The experiment followed a randomized complete block design with four treatments and five replicates: T1: control; T2: application of 140 g a.i ha-1 of lactofen in phenological stage V3; T3: application of 140 g a.i ha-1 of lactofen in phenological stage V6; and T4: application of 70 g a.i ha-1 of lactofen in phenological stage V3 + 70 g a.i ha-1 of lactofen in phenological stage V6. The interception of photosynthetically active radiation in the lower layer increased in all treatments. Lactofen application increased the percent area covered and the number of phytosanitary products spray droplets per cm² in the middle and lower layers of the plants. The lower third of the plants experienced the greatest effect of the treatments with regard to the number of pods, grains and grain weight, with treatment T2 presenting significant increases. The use of lactofen as a growth inhibitor at the beginning of pod development in soybean caused changes to plant architecture and root development, consequently enhanced the productive efficiency of the plant, primarily due to increased grain production in the lower layer. Future research using lactofen in different phenological stages and cultivars may provide more insights in to the performance of this growth inhibitor in soybean

Genome-wide, evolutionary, and functional analyses of ascorbate peroxidase (APX) family in Poaceae species

Abstract Ascorbate peroxidases (APXs) are heme peroxidases involved in the control of hydrogen peroxide levels and signal transduction pathways related to development and stress responses. Here, a total of 238 APX, 30 APX-related (APX-R), and 34 APX-like (APX-L) genes were identified from 24 species from the Poaceae family. Phylogenetic analysis of APX indicated five distinct clades, equivalent to cytosolic (cAPX), peroxisomal (pAPX), mitochondrial (mitAPX), stromal (sAPX), and thylakoidal (tAPX) isoforms. Duplication events contributed to the expansion of this family and the divergence times. Different from other APX isoforms, the emergence of Poaceae mitAPXs occurred independently after eudicot and monocot divergence. Our results showed that the constitutive silencing of mitAPX genes is not viable in rice plants, suggesting that these isoforms are essential for rice regeneration or development. We also obtained rice plants silenced individually to sAPX isoforms, demonstrating that, different to plants double silenced to both sAPX and tAPX or single silenced to tAPX previously obtained, these plants do not show changes in the total APX activity and hydrogen peroxide content in the shoot. Among rice plants silenced to different isoforms, plants silenced to cAPX showed a higher decrease in total APX activity and an increase in hydrogen peroxide levels. These results suggest that the cAPXs are the main isoforms responsible for regulating hydrogen peroxide levels in the cell, whereas in the chloroplast, this role is provided mainly by the tAPX isoform. In addition to broadening our understanding of the core components of the antioxidant defense in Poaceae species, the present study also provides a platform for their functional characterization

Stromal Ascorbate Peroxidase (<i>OsAPX7</i>) Modulates Drought Stress Tolerance in Rice (<i>Oryza sativa</i>)

Chloroplast ascorbate peroxidases exert an important role in the maintenance of hydrogen peroxide levels in chloroplasts by using ascorbate as the specific electron donor. In this work, we performed a functional study of the stromal APX in rice (OsAPX7) and demonstrated that silencing of OsAPX7 did not impact plant growth, redox state, or photosynthesis parameters. Nevertheless, when subjected to drought stress, silenced plants (APX7i) show a higher capacity to maintain stomata aperture and photosynthesis performance, resulting in a higher tolerance when compared to non-transformed plants. RNA-seq analyses indicate that the silencing of OsAPX7 did not lead to changes in the global expression of genes related to reactive oxygen species metabolism. In addition, the drought-mediated induction of several genes related to the proteasome pathway and the down-regulation of genes related to nitrogen and carotenoid metabolism was impaired in APX7i plants. During drought stress, APX7i showed an up-regulation of genes encoding flavonoid and tyrosine metabolism enzymes and a down-regulation of genes related to phytohormones signal transduction and nicotinate and nicotinamide metabolism. Our results demonstrate that OsAPX7 might be involved in signaling transduction pathways related to drought stress response, contributing to the understanding of the physiological role of chloroplast APX isoforms in rice